##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630695.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810940
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.034025945199396	33.0	31.0	34.0	30.0	34.0
2	32.134908871186525	33.0	31.0	34.0	30.0	34.0
3	32.192260833107255	34.0	31.0	34.0	30.0	34.0
4	35.80472291414901	37.0	35.0	37.0	35.0	37.0
5	35.795380669346684	37.0	35.0	37.0	35.0	37.0
6	35.90773793375589	37.0	35.0	37.0	35.0	37.0
7	35.83477445926949	37.0	35.0	37.0	35.0	37.0
8	35.89047155153279	37.0	35.0	37.0	35.0	37.0
9	37.51506276666584	39.0	37.0	39.0	35.0	39.0
10	37.45955311120428	39.0	37.0	39.0	35.0	39.0
11	37.57514735985399	39.0	37.0	39.0	35.0	39.0
12	37.54786420696969	39.0	37.0	39.0	35.0	39.0
13	37.580670579820946	39.0	37.0	39.0	35.0	39.0
14	38.76972895651959	40.0	38.0	41.0	35.0	41.0
15	38.746568180136634	40.0	38.0	41.0	35.0	41.0
16	38.650077687621774	40.0	38.0	41.0	34.0	41.0
17	38.741739216218214	40.0	38.0	41.0	35.0	41.0
18	38.76902236910252	40.0	38.0	41.0	35.0	41.0
19	38.84667299676918	40.0	38.0	41.0	35.0	41.0
20	38.82157742866303	40.0	38.0	41.0	35.0	41.0
21	38.76624411177153	40.0	38.0	41.0	35.0	41.0
22	38.62683305793277	40.0	38.0	41.0	34.0	41.0
23	38.52632253927541	40.0	38.0	41.0	34.0	41.0
24	38.41743285569832	40.0	38.0	41.0	34.0	41.0
25	38.335927442227536	40.0	37.0	41.0	34.0	41.0
26	38.09283917429156	40.0	37.0	41.0	34.0	41.0
27	37.87595753076676	40.0	36.0	41.0	33.0	41.0
28	37.68928280760599	40.0	36.0	41.0	33.0	41.0
29	37.526894714775445	40.0	36.0	41.0	33.0	41.0
30	37.31705921523171	39.0	35.0	41.0	33.0	41.0
31	37.12426936641428	39.0	35.0	41.0	32.0	41.0
32	36.88469060596345	39.0	35.0	41.0	32.0	41.0
33	36.631486916417984	39.0	35.0	41.0	31.0	41.0
34	36.44863491750314	39.0	35.0	41.0	31.0	41.0
35	36.233595580437516	39.0	35.0	41.0	30.0	41.0
36	36.05375860112955	38.0	35.0	41.0	30.0	41.0
37	35.76678669198708	38.0	35.0	41.0	29.0	41.0
38	35.44396009569142	38.0	35.0	40.0	27.0	41.0
39	35.159534614151475	38.0	35.0	40.0	25.0	41.0
40	34.75678595210496	38.0	34.0	40.0	23.0	41.0
41	34.45171775963696	38.0	34.0	40.0	21.0	41.0
42	34.07517818827534	37.0	33.0	40.0	18.0	41.0
43	33.227283152884304	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	8.0
12	13.0
13	6.0
14	3.0
15	4.0
16	7.0
17	16.0
18	36.0
19	98.0
20	203.0
21	376.0
22	730.0
23	1317.0
24	2015.0
25	3267.0
26	4751.0
27	7004.0
28	9894.0
29	13519.0
30	16773.0
31	20812.0
32	25384.0
33	31626.0
34	41547.0
35	53186.0
36	74779.0
37	122903.0
38	165396.0
39	215259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.63368436629097	19.578760450834835	12.010383012306706	19.77717217056749
2	19.26381729844378	22.89700840999334	32.980985029718596	24.85818926184428
3	23.755025032678127	26.398377191900757	27.38402347892569	22.462574296495426
4	13.818778208005526	15.967149234222013	31.901373714454827	38.312698843317634
5	13.573383974153453	40.482526450785514	30.338372752608084	15.605716822452957
6	32.9767923644166	32.04392433472267	17.089920339359264	17.88936296150147
7	27.06414777912053	33.33637507090537	21.05938787086591	18.540089279108194
8	31.472242089427084	28.949614028164845	20.083729992354552	19.49441389005352
9	28.625792290428393	11.420080400522851	22.214097220509533	37.74003008853923
10	20.769107455545416	27.08942708461785	28.377191900757147	23.764273559079587
11	33.902384886674724	22.445803635287444	19.32843366956865	24.323377808469186
12	22.30608922978272	29.38293831849459	27.560362048980195	20.7506104027425
13	30.05586109946482	19.32794041482724	28.47941894591462	22.136779539793327
14	23.995363405430734	22.344193158556735	28.05447998618887	25.60596344982366
15	27.350975411251138	25.015784151725157	26.62564431400597	21.007596123017734
16	23.72037388709399	25.364268626532173	28.383727526080843	22.531629960292992
17	21.29812316570893	27.51695563173601	27.847066367425455	23.337854835129605
18	21.33277431129307	22.584161590253284	31.950575874910598	24.13248822354305
19	21.283695464522655	25.815966655979484	32.72671221052113	20.173625668976744
20	22.741386539078107	22.846573112684045	33.05053394825757	21.36150639998027
21	24.825511135225785	23.934569758551806	31.35743704836363	19.88248205785878
22	24.379485535304706	23.493353392359484	31.338816681875354	20.788344390460452
23	21.951192443337362	24.200310750487088	31.617628924458035	22.230867881717515
24	20.76096875231213	26.323895725947665	32.10212839420919	20.813007127531012
25	20.858509877426197	26.058771302439144	32.024564086122275	21.05815473401238
26	21.280859249759537	27.288702000147975	31.16334130761832	20.267097442474167
27	21.711840580067577	26.045330110735687	31.466569659900856	20.776259649295877
28	19.807260709793574	25.807088070634077	33.171997928330086	21.213653291242263
29	19.47468370039707	26.914321651416873	33.25622117542605	20.35477347276001
30	18.923471526870053	28.34932300786741	32.9326460650603	19.794559400202235
31	20.17226921843786	28.299134337928823	30.668853429353586	20.859743014279726
32	19.389597257503638	27.933139319801715	31.043850346511455	21.633413076183196
33	18.772905517054284	27.11791254593435	32.41448195920783	21.694699977803538
34	20.664660764051597	27.06649073914223	31.96993612351099	20.298912373295188
35	19.98594223986978	27.358127605001602	31.84649912447283	20.80943103065578
36	18.053370163020695	28.634300934717732	30.73371642784916	22.57861247441241
37	19.959306483833576	27.93757861247441	30.807704639060844	21.29541026463117
38	19.342368116013514	26.435617924877302	31.781266184921204	22.44074777418798
39	20.080400522850024	27.35307174390214	31.09662860384245	21.46989912940538
40	18.979579253705577	27.434088835178926	32.15453671048413	21.431795200631363
41	17.80452314597874	28.214664463462153	32.157372925247245	21.82343946531186
42	18.727649394529806	27.74459269489728	31.757713271018815	21.770044639554097
43	18.34069104989272	26.583101092559254	31.278392976052483	23.797814881495547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1590.0
1	1205.5
2	821.0
3	1653.0
4	2485.0
5	2485.0
6	3593.0
7	4701.0
8	4566.5
9	4432.0
10	6638.0
11	8844.0
12	8844.0
13	15501.0
14	22158.0
15	27981.0
16	33804.0
17	30434.5
18	27065.0
19	27065.0
20	27835.0
21	28605.0
22	20761.5
23	12918.0
24	11600.0
25	10282.0
26	10282.0
27	10603.5
28	10925.0
29	11206.5
30	11488.0
31	12241.0
32	12994.0
33	12994.0
34	14612.0
35	16230.0
36	18153.0
37	20076.0
38	24109.0
39	28142.0
40	28142.0
41	35246.5
42	42351.0
43	44218.5
44	46086.0
45	49030.0
46	51974.0
47	51974.0
48	54521.0
49	57068.0
50	53482.0
51	49896.0
52	50862.5
53	51829.0
54	51829.0
55	51439.0
56	51049.0
57	51208.5
58	51368.0
59	47690.0
60	44012.0
61	44012.0
62	40041.5
63	36071.0
64	29903.0
65	23735.0
66	20472.5
67	17210.0
68	17210.0
69	14073.0
70	10936.0
71	9179.0
72	7422.0
73	5493.5
74	3565.0
75	3565.0
76	2787.5
77	2010.0
78	2162.0
79	2314.0
80	1824.5
81	1335.0
82	1335.0
83	1175.5
84	1016.0
85	996.5
86	977.0
87	806.5
88	636.0
89	636.0
90	557.5
91	479.0
92	258.0
93	37.0
94	20.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	810940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.814722606253916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.30032847906068	29.833781574873914
2	8.082017530726548	5.789104319237051
3	2.476103334397942	2.66042862197648
4	1.20437866337296	1.725379509663737
5	0.8288890956559692	1.4843216516133604
6	0.5758920262368451	1.2375247902495663
7	0.4127554500609502	1.0347905363706698
8	0.30020681024319745	0.8601458906694739
9	0.265947235250845	0.857234381256825
>10	1.974950918522772	14.206052134120759
>50	0.28684604548501796	7.160374366479942
>100	0.26230861130165545	18.663265067305073
>500	0.022463846817664827	5.700945231158507
>1k	0.005529562293579034	2.9051192062467988
>5k	3.4559764334868965E-4	1.0945408862357993
>10k+	0.001036792930046069	4.786991832542071
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14146	1.7443953930007152	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13377	1.6495671689644116	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11152	1.3751942190544308	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8843	1.0904629195748143	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2623	0.3234517966803956	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2133	0.2630280908575234	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1982	0.2444077243692505	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1959	0.24157150960613608	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1719	0.21197622512146397	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1439	0.17744839322267986	No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC	1393	0.17177596369645104	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1293	0.159444595161171	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1260	0.15537524354452859	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1214	0.14970281401829974	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1190	0.14674328556983254	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1129	0.1392211507633117	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1075	0.13256221175426047	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1037	0.12787629171085407	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1018	0.12553333168915087	No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTT	1007	0.12417688115027006	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	992	0.12232717586997804	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	983	0.12121735270180284	No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC	971	0.11973758847756924	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	970	0.11961427479221644	No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAAC	953	0.11751794214121883	No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCT	947	0.11677806002910202	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	943	0.11628480528769083	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.11517498211951563	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGC	911	0.1123387673564012	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	896	0.11048906207610922	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	893	0.11011912102005081	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	883	0.10888598416652279	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	873	0.10765284731299479	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	859	0.10592645571805559	No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCA	859	0.10592645571805559	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	832	0.10259698621352999	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	830	0.10235035884282437	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	825	0.10173379041606037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.865094828224036E-4	0.0
3	0.0	0.0	0.0	0.0016030779095864058	0.0
4	0.0	0.0	0.0	0.003082842133820011	0.0
5	0.0	0.0	0.0	0.003329469504525612	0.0
6	0.0	0.0	0.0	0.003329469504525612	0.0
7	0.0	0.0	0.0	0.0034527831898784125	0.0
8	0.0	0.0	0.0	0.003822724245936814	0.0
9	0.0	0.0	0.0	0.006782252694404025	0.0
10	0.0	0.0	0.0	0.01109823168175204	0.0
11	0.0	0.0	0.0	0.01640072015192246	0.0
12	0.0	0.0	0.0	0.025895873924088094	0.0
13	0.0	0.0	0.0	0.029348657113966507	0.0
14	0.0	0.0	0.0	0.03798061508866254	0.0
15	0.0	0.0	0.0	0.04858559202900338	0.0
16	0.0	0.0	0.0	0.0805238365353787	0.0
17	0.0	0.0	0.0	0.12158729375786125	0.0
18	0.0	0.0	0.0	0.1351517991466693	0.0
19	0.0	0.0	0.0	0.1603077909586406	0.0
20	0.0	0.0	0.0	0.17855821639085506	0.0
21	0.0	0.0	0.0	0.22541741682491923	0.0
22	0.0	0.0	0.0	0.3084075270673539	0.0
23	0.0	0.0	0.0	0.427651860803512	0.0
24	0.0	0.0	0.0	0.6186647594149999	0.0
25	0.0	0.0	0.0	0.6678669198707673	0.0
26	0.0	0.0	0.0	0.7326066046809875	0.0
27	0.0	0.0	0.0	0.791673859964979	0.0
28	0.0	0.0	0.0	0.8860088292598712	0.0
29	0.0	0.0	0.0	1.0117887883197276	0.0
30	0.0	0.0	0.0	1.2262312871482477	0.0
31	0.0	0.0	0.0	1.7871852418181369	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATGA	35	8.8671345E-4	26.42857	3
AAGACGG	360	0.0	23.125002	5
CCGCTTA	80	2.723391E-8	23.125	25
ACTGTAG	40	0.0019306997	23.125	8
AGCGTCA	90	3.8235157E-9	22.611113	3
ATATACG	75	3.7379505E-7	22.2	6
ACGCTAT	75	3.7379505E-7	22.2	10
CGCTTAT	75	3.7379505E-7	22.2	26
TCTAATA	120	1.0913936E-11	21.583332	2
TTGGCGC	95	7.1377144E-9	21.421053	10
GCGCTTG	105	9.822543E-10	21.142857	6
TACTGGT	115	1.3460522E-10	20.913044	7
TACGCTA	80	6.954924E-7	20.8125	9
TTGCTAT	45	0.0038248503	20.555557	1
CGTCTCT	45	0.0038248503	20.555557	20
CAGCGTC	100	1.2883902E-8	20.35	2
GACGGAC	400	0.0	20.35	7
GCAGCGT	100	1.2883902E-8	20.35	1
ACGGACC	410	0.0	20.304876	8
CAAGACG	415	0.0	20.060242	4
>>END_MODULE