FastQCFastQC Report
Fri 10 Feb 2017
ERR1630694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729168
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC17880.24521098018563622No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT14210.19487964364865162No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA13440.1843196629583306No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA11900.16319970157768854No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG11150.15291400610010314No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG11000.15085686700458603No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG10850.14879972790906895No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG10850.14879972790906895No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG10420.14290259583525333No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG9940.13631975072959868No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG9620.1319311873258289No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG9290.1274054813156913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8230.11286836504070394No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA7850.10765694599872731No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG7570.10381695302042876No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA7460.1023083843503829No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT7430.10189695653127949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG250.00549504329.5999985
CGAACGA556.2564686E-726.9090916
GTATAGA451.3225642E-424.6666661
GCTTTAT3350.022.641791
ACGAACG705.0977033E-621.14285715
GTGCTAT555.140614E-420.1818181
GAGCGAA1101.7516868E-920.18181816
GACGGAC1202.382876E-1020.0416687
TCCGATA759.258152E-619.7333348
CGTATGC1700.019.58823631
TTCCGAT851.2440305E-619.5882367
GCGAAAG1153.0449883E-919.30434818
CTTTATT4050.018.7283952
TAACGAA801.6153495E-518.513
GCGCAAG1306.9485395E-1018.51
TATTGAG4200.018.55
ACGGACC1306.9485395E-1018.58
AACGAGA801.6153495E-518.518
AGCGAAA1205.164111E-918.517
ATAACGA801.6153495E-518.512
Produced by FastQC (version 0.11.5)