FastQCFastQC Report
Fri 10 Feb 2017
ERR1630693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720448
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97370.3579189898134425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95270.3501996729950361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76660.2817918225233491No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56630.2081642435363587No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG32160.11821582327616628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA2100.016.73809436
TTGTACG803.3846922E-416.18754
AAGACGG4500.015.6222225
GGTATCA52200.015.2040221
TCTAGCG2251.8189894E-1214.828
GACGGAC4400.014.7159097
ACGGACC4550.014.63736258
CGCAAGA5050.014.28712752
GTACTAG1702.44072E-814.1470591
CGGACCA4700.013.7765959
TTACACT3500.013.7428584
AGACGGA5250.013.7428576
TAGACAG3250.013.6615385
CGATCGC1907.1377144E-913.63157919
CGAAAGC4750.013.63157819
TCTTATA25000.013.61599937
GTAGAAC10600.013.6132083
CAAGACG5600.013.5446424
GGTCTAG2051.4260877E-913.5365861
TAGAACA10700.013.4859814
Produced by FastQC (version 0.11.5)