##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630693.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2720448
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20238578351801	33.0	31.0	34.0	30.0	34.0
2	32.383752234926014	34.0	31.0	34.0	30.0	34.0
3	32.489140391582566	34.0	31.0	34.0	30.0	34.0
4	36.043245818335805	37.0	35.0	37.0	35.0	37.0
5	35.991080880796105	37.0	35.0	37.0	35.0	37.0
6	36.06559434328464	37.0	35.0	37.0	35.0	37.0
7	36.0156202213753	37.0	35.0	37.0	35.0	37.0
8	36.019181767120706	37.0	35.0	37.0	35.0	37.0
9	37.75367182169995	39.0	37.0	39.0	35.0	39.0
10	37.677667428305924	39.0	37.0	39.0	35.0	39.0
11	37.760178103018326	39.0	37.0	39.0	35.0	39.0
12	37.7195888324276	39.0	37.0	39.0	35.0	39.0
13	37.74704644235067	39.0	37.0	39.0	35.0	39.0
14	39.091330913143715	40.0	38.0	41.0	36.0	41.0
15	39.10768263168419	40.0	38.0	41.0	36.0	41.0
16	39.075700766932506	40.0	38.0	41.0	36.0	41.0
17	39.06784764862258	40.0	38.0	41.0	36.0	41.0
18	39.0501332868704	40.0	38.0	41.0	36.0	41.0
19	39.12104035805867	40.0	39.0	41.0	36.0	41.0
20	39.09001789411156	40.0	39.0	41.0	36.0	41.0
21	39.05214692580046	40.0	39.0	41.0	36.0	41.0
22	38.988496012421486	40.0	39.0	41.0	35.0	41.0
23	38.9485066430235	40.0	38.0	41.0	35.0	41.0
24	38.906965323358506	40.0	38.0	41.0	35.0	41.0
25	38.86185620897734	40.0	38.0	41.0	35.0	41.0
26	38.746357217634745	40.0	38.0	41.0	35.0	41.0
27	38.62905411167572	40.0	38.0	41.0	34.0	41.0
28	38.551523131484224	40.0	38.0	41.0	34.0	41.0
29	38.489292204813324	40.0	38.0	41.0	34.0	41.0
30	38.414021146517044	40.0	38.0	41.0	34.0	41.0
31	38.36540488919472	40.0	38.0	41.0	34.0	41.0
32	38.27649967946456	40.0	38.0	41.0	34.0	41.0
33	38.211489063566	40.0	38.0	41.0	34.0	41.0
34	38.15054432211165	40.0	38.0	41.0	34.0	41.0
35	38.10702943044675	40.0	38.0	41.0	34.0	41.0
36	38.06661292551815	40.0	38.0	41.0	33.0	41.0
37	37.94469330051521	40.0	37.0	41.0	33.0	41.0
38	37.804750173500835	40.0	37.0	41.0	33.0	41.0
39	37.74967174524196	40.0	37.0	41.0	33.0	41.0
40	37.590626617380664	40.0	37.0	41.0	33.0	41.0
41	37.52981053120662	40.0	37.0	41.0	33.0	41.0
42	37.407294313289576	40.0	37.0	41.0	32.0	41.0
43	36.65804382219399	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	8.0
11	6.0
12	6.0
13	3.0
14	8.0
15	4.0
16	11.0
17	21.0
18	46.0
19	110.0
20	210.0
21	489.0
22	821.0
23	1541.0
24	2641.0
25	4314.0
26	6727.0
27	10425.0
28	15437.0
29	22010.0
30	30482.0
31	40955.0
32	54113.0
33	72475.0
34	97842.0
35	133710.0
36	193016.0
37	307870.0
38	586324.0
39	1138818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18706036652787	19.007861940386288	11.882123826663843	24.922953866422002
2	18.028243877479003	20.98062524995883	36.08585791751946	24.905272955042697
3	19.959175841626084	23.848388206648316	29.85798662573223	26.334449325993365
4	14.043789846378244	15.357066189098267	34.90204554543958	35.697098419083915
5	13.728069788505422	37.952609276119226	34.64131643023502	13.67800450514033
6	34.59985267132472	34.941708130425575	16.095216670195498	14.363222528054203
7	28.62010227727198	30.93578704683934	21.958368621638787	18.485742054249886
8	26.787720257839887	34.4189633472134	20.243577528407087	18.549738866539627
9	26.484461382831064	13.80434399040158	20.013100783400382	39.698093843366976
10	16.180276189804033	27.60306390712118	33.758557414072975	22.45810248900181
11	34.73710947608629	22.020564260004235	22.42961453407674	20.812711729832735
12	21.183385971722306	25.981272202225515	29.872285741172043	22.963056084880137
13	29.483305690827393	20.300443162302678	26.273099136612792	23.943152010257133
14	22.41921183569765	21.20485302420778	26.232333792081302	30.143601348013267
15	25.252458418613404	28.15565671536453	23.377767191286143	23.214117674735927
16	24.57933399204837	27.12509116145576	24.70214464676406	23.59343019973181
17	23.10939227656621	27.240108982050014	26.203147422777423	23.447351318606348
18	22.59613122544522	25.797368668689863	27.916762239160608	23.68973786670431
19	23.56299403627638	26.23207648152069	27.749657409367867	22.45527207283506
20	23.99413625991013	25.357257334086153	27.8940821511751	22.754524254828617
21	23.93035999952949	26.076513868304048	26.792315089279413	23.200811042887054
22	23.916097642741196	25.67389635824688	26.89748159126732	23.512524407744607
23	23.21555126214506	26.163301044533842	27.174237478532948	23.446910214788154
24	23.81578328275343	26.267548580233846	26.966330545557206	22.950337591455526
25	23.821223563177828	25.764175606370713	27.101639141788414	23.312961688663044
26	23.569243007034135	26.214763156656552	27.031136048180297	23.184857788129015
27	23.688524831204273	25.92958953819371	26.703984049685936	23.67790158091608
28	23.078809078504715	26.099635060107744	27.405853741736657	23.41570211965088
29	22.854912132119416	26.71622468064084	27.375123509069095	23.053739678170654
30	22.592124532429953	27.233602700731645	27.231470698943706	22.9428020678947
31	23.3371121227092	26.581614498788436	26.96838903004211	23.112884348460252
32	22.55753464135319	26.55103130072694	27.241983673277343	23.649450384642527
33	22.756693015268073	26.185797339261768	27.524804738043144	23.532704907427014
34	23.2258436845696	26.14297351024537	27.216068823958405	23.41511398122662
35	23.168132601689134	26.34665319829675	27.35376673253817	23.131447467475947
36	22.794885254193428	26.50975133507422	27.208092196579386	23.487271214152962
37	23.442388900651657	25.508335391817816	27.365676535629426	23.683599171901097
38	22.770073164419976	25.77656327193168	27.99902810125391	23.45433546239443
39	22.78201972616275	25.655039170019055	28.082617274801798	23.4803238290164
40	22.423512597925047	25.35865416284377	28.61580151504458	23.602031724186602
41	21.82294974945303	25.91448173242054	28.816724304232245	23.445844213894183
42	22.449795033759145	25.364131201919683	28.590879149316585	23.595194615004587
43	21.769796739360576	25.330239725221727	28.579226656785938	24.320736878631756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	267.5
2	335.0
3	1095.5
4	1856.0
5	1856.0
6	2574.5
7	3293.0
8	3359.5
9	3426.0
10	5014.5
11	6603.0
12	6603.0
13	11009.5
14	15416.0
15	21159.5
16	26903.0
17	26455.0
18	26007.0
19	26007.0
20	30111.5
21	34216.0
22	34522.5
23	34829.0
24	39113.0
25	43397.0
26	43397.0
27	48545.5
28	53694.0
29	65359.5
30	77025.0
31	83843.5
32	90662.0
33	90662.0
34	99458.5
35	108255.0
36	116125.0
37	123995.0
38	138441.0
39	152887.0
40	152887.0
41	161231.5
42	169576.0
43	166019.0
44	162462.0
45	169206.5
46	175951.0
47	175951.0
48	181419.5
49	186888.0
50	190323.5
51	193759.0
52	199518.0
53	205277.0
54	205277.0
55	186878.0
56	168479.0
57	158986.5
58	149494.0
59	137865.5
60	126237.0
61	126237.0
62	117194.0
63	108151.0
64	96154.0
65	84157.0
66	73622.5
67	63088.0
68	63088.0
69	54239.0
70	45390.0
71	38830.0
72	32270.0
73	25811.0
74	19352.0
75	19352.0
76	15338.5
77	11325.0
78	9171.0
79	7017.0
80	5498.0
81	3979.0
82	3979.0
83	2957.0
84	1935.0
85	1607.5
86	1280.0
87	1093.0
88	906.0
89	906.0
90	657.5
91	409.0
92	229.5
93	50.0
94	31.5
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2720448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.435982382959715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05098584526284	45.20962002360164
2	10.212563025345748	11.11861801865174
3	2.8309891381216428	4.623230245474201
4	1.2042836436320177	2.6222545283535617
5	0.633358105556252	1.7238735338082438
6	0.38353791240179586	1.2526957825620781
7	0.27425272711321574	1.045045163512957
8	0.19678295420010894	0.8569658742483119
9	0.14128477558864347	0.6921877999431431
>10	0.8639919414709211	8.880409357748565
>50	0.09613490245144699	3.6794211304653204
>100	0.09949546968255549	11.502451980275481
>500	0.009220769491932344	3.35459412159529
>1k	0.002847590578387573	2.235707141547918
>5k	2.711991027035784E-4	1.2029252982116894
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9737	0.3579189898134425	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9527	0.3501996729950361	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7666	0.2817918225233491	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5663	0.2081642435363587	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3216	0.11821582327616628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.675865151622086E-5	0.0
2	0.0	0.0	0.0	6.984143788081962E-4	0.0
3	0.0	0.0	0.0	0.0011762768485190675	0.0
4	0.0	0.0	0.0	0.0019482085303597055	0.0
5	0.0	0.0	0.0	0.00213200178794081	0.0
6	0.0	0.0	0.0	0.002279036394005693	0.0
7	0.0	0.0	0.0	0.0027201402122003435	0.0
8	0.0	0.0	0.0	0.0029406921212976685	0.0
9	7.351730303244172E-5	0.0	0.0	0.005109452560754699	0.0
10	7.351730303244172E-5	0.0	0.0	0.0075722822123414964	0.0
11	7.351730303244172E-5	0.0	0.0	0.008785317712376785	0.0
12	7.351730303244172E-5	0.0	0.0	0.00985131860634719	0.0
13	7.351730303244172E-5	0.0	0.0	0.010402698379090502	0.0
14	7.351730303244172E-5	0.0	0.0	0.011431940621544687	0.0
15	7.351730303244172E-5	0.0	0.0	0.013380149151904393	0.0
16	7.351730303244172E-5	0.0	0.0	0.016504634530783165	0.0
17	7.351730303244172E-5	0.0	0.0	0.02007022372785659	0.0
18	7.351730303244172E-5	0.0	0.0	0.022496294727927166	0.0
19	1.1027595454866258E-4	0.0	0.0	0.025841332015903263	0.0
20	1.1027595454866258E-4	0.0	0.0	0.028340920319006282	0.0
21	1.1027595454866258E-4	0.0	0.0	0.033340096925212316	0.0
22	1.1027595454866258E-4	0.0	0.0	0.04289734631942974	0.0
23	1.1027595454866258E-4	7.351730303244172E-5	0.0	0.05664508198649634	0.0
24	1.1027595454866258E-4	7.351730303244172E-5	0.0	0.07689909897193403	0.0
25	1.1027595454866258E-4	7.351730303244172E-5	0.0	0.08267020725998071	0.0
26	1.1027595454866258E-4	7.351730303244172E-5	0.0	0.09402863057849295	0.0
27	1.1027595454866258E-4	7.351730303244172E-5	0.0	0.10678388265462159	0.0
28	1.1027595454866258E-4	1.1027595454866258E-4	0.0	0.13453666454936833	0.0
29	1.1027595454866258E-4	1.4703460606488343E-4	0.0	0.17842649445973605	0.0
30	1.1027595454866258E-4	1.4703460606488343E-4	0.0	0.24341579034041452	0.0
31	1.1027595454866258E-4	1.4703460606488343E-4	0.0	0.4897722728021267	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	210	0.0	16.738094	36
TTGTACG	80	3.3846922E-4	16.1875	4
AAGACGG	450	0.0	15.622222	5
GGTATCA	5220	0.0	15.204022	1
TCTAGCG	225	1.8189894E-12	14.8	28
GACGGAC	440	0.0	14.715909	7
ACGGACC	455	0.0	14.6373625	8
CGCAAGA	505	0.0	14.2871275	2
GTACTAG	170	2.44072E-8	14.147059	1
CGGACCA	470	0.0	13.776595	9
TTACACT	350	0.0	13.742858	4
AGACGGA	525	0.0	13.742857	6
TAGACAG	325	0.0	13.661538	5
CGATCGC	190	7.1377144E-9	13.631579	19
CGAAAGC	475	0.0	13.631578	19
TCTTATA	2500	0.0	13.615999	37
GTAGAAC	1060	0.0	13.613208	3
CAAGACG	560	0.0	13.544642	4
GGTCTAG	205	1.4260877E-9	13.536586	1
TAGAACA	1070	0.0	13.485981	4
>>END_MODULE