FastQCFastQC Report
Fri 10 Feb 2017
ERR1630691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630732
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC59560.22640086485434474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56640.21530129256800007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.20606431974066536No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT50270.19108749960087154No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA49380.18770441078756786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48390.18394119963569075No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA47430.18029202518538567No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG36740.13965694719188423No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG34680.13182642701727124No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG34070.12950768075197322No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.12342572333479808No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG31600.12011865898920908No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA30830.11719171698219355No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG28940.11000740478315542No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG28310.1076126340501427No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG28200.1071944994777119No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA26790.1018347745038263No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA26710.10153067663296755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.018.5315151
GCTTTAT14650.018.310581
AAGACGG3850.018.259745
GACGGAC3550.018.2394377
ACGGACC3750.017.2666668
CAAGACG5300.016.405664
AGACGGA4600.016.0869566
CGGACCA4200.015.4166669
TATTGAG16450.015.4072955
CGAATTA1455.3536496E-815.31034515
GAATCGT750.004106276714.86
CGCAAGA5200.014.2307692
TATACTG4050.014.1604945
ACGCTAT1702.440538E-814.14705910
CTTATAC12650.014.03952637
GAGCGAA4650.013.92473116
GCGAAAG4800.013.87500118
TTTATTG19200.013.8750013
TAATACT4400.013.8754
ACCTAGG18200.013.8241751
Produced by FastQC (version 0.11.5)