##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2630732
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.126467082165725	33.0	31.0	34.0	30.0	34.0
2	32.29794178958556	34.0	31.0	34.0	30.0	34.0
3	32.41717514364823	34.0	31.0	34.0	30.0	34.0
4	35.970099576847815	37.0	35.0	37.0	35.0	37.0
5	35.92160394901495	37.0	35.0	37.0	35.0	37.0
6	35.99034907394596	37.0	35.0	37.0	35.0	37.0
7	35.94246772381223	37.0	35.0	37.0	35.0	37.0
8	35.93582926729138	37.0	35.0	37.0	35.0	37.0
9	37.65731210932927	39.0	37.0	39.0	35.0	39.0
10	37.574245875292505	39.0	37.0	39.0	35.0	39.0
11	37.65714257476626	39.0	37.0	39.0	35.0	39.0
12	37.60721920742972	39.0	37.0	39.0	35.0	39.0
13	37.648205898586404	39.0	37.0	39.0	35.0	39.0
14	38.97181696957349	40.0	38.0	41.0	36.0	41.0
15	38.99072425469413	40.0	38.0	41.0	36.0	41.0
16	38.955269864053044	40.0	38.0	41.0	36.0	41.0
17	38.938171581141674	40.0	38.0	41.0	36.0	41.0
18	38.92423325523087	40.0	38.0	41.0	35.0	41.0
19	38.971940129211184	40.0	38.0	41.0	35.0	41.0
20	38.94577212730145	40.0	38.0	41.0	35.0	41.0
21	38.89995104024279	40.0	38.0	41.0	35.0	41.0
22	38.83842823974468	40.0	38.0	41.0	35.0	41.0
23	38.800125212298326	40.0	38.0	41.0	35.0	41.0
24	38.75749145104861	40.0	38.0	41.0	35.0	41.0
25	38.714442976327504	40.0	38.0	41.0	34.0	41.0
26	38.59932482670222	40.0	38.0	41.0	34.0	41.0
27	38.48142570204795	40.0	38.0	41.0	34.0	41.0
28	38.38942621293237	40.0	38.0	41.0	34.0	41.0
29	38.34386284881926	40.0	38.0	41.0	34.0	41.0
30	38.27606308814429	40.0	38.0	41.0	34.0	41.0
31	38.24414801659766	40.0	38.0	41.0	34.0	41.0
32	38.177032856254456	40.0	38.0	41.0	33.0	41.0
33	38.10863288240687	40.0	38.0	41.0	33.0	41.0
34	38.06358116296149	40.0	38.0	41.0	33.0	41.0
35	38.01112161938198	40.0	38.0	41.0	33.0	41.0
36	37.99594751574847	40.0	37.0	41.0	33.0	41.0
37	37.881499901928436	40.0	37.0	41.0	33.0	41.0
38	37.761653790655984	40.0	37.0	41.0	33.0	41.0
39	37.71932070617608	40.0	37.0	41.0	33.0	41.0
40	37.55611213913086	40.0	37.0	41.0	32.0	41.0
41	37.52890830384851	40.0	37.0	41.0	32.0	41.0
42	37.408227063798215	40.0	37.0	41.0	32.0	41.0
43	36.64373718037413	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	3.0
10	6.0
11	9.0
12	6.0
13	6.0
14	5.0
15	6.0
16	10.0
17	23.0
18	46.0
19	91.0
20	218.0
21	463.0
22	917.0
23	1586.0
24	2846.0
25	4818.0
26	7248.0
27	11295.0
28	16614.0
29	23757.0
30	32434.0
31	43418.0
32	56003.0
33	74254.0
34	99019.0
35	134007.0
36	190455.0
37	297358.0
38	581851.0
39	1051953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81942668428407	18.651196701146297	11.399754897116088	25.129621717453542
2	19.24365537804687	20.316854776541284	33.72795100375105	26.711538841660797
3	21.057105018679213	22.787155818228538	28.40296921161106	27.75276995148118
4	14.49254428045122	14.300354426068484	34.57387525601239	36.6332260374679
5	14.655730800400802	36.69127071856806	34.45428876829719	14.198709712733946
6	36.457457468111535	34.182501296217175	14.907789923108853	14.452251312562437
7	30.18703539547168	29.400866374834077	21.127503675783014	19.28459455391123
8	28.67627717304537	31.832166864583698	19.66726371215312	19.82429225021781
9	27.12024638009497	13.439757451538203	19.355753455692177	40.084242712674644
10	17.374403778111947	26.429716139842448	32.709527234245066	23.486352847800536
11	36.44092214638359	21.334632338071685	21.264195668734025	20.960249846810697
12	23.634410498674892	24.774207330887375	28.035200849041253	23.556181321396476
13	31.300071615048587	19.35932660567477	24.43129896926027	24.909302810016378
14	23.331338958130285	20.22581547645294	24.893831830836437	31.54901373458034
15	26.222169342981346	27.744635333435713	21.79781140762343	24.235383915959513
16	25.8185174316502	25.981399853728927	23.413217309859004	24.78686540476187
17	24.725437634848397	25.664833970164956	24.597260382281434	25.01246801270521
18	24.0772150108791	24.669825736715104	26.54432302492234	24.708636227483453
19	25.58261350833152	24.496071815753183	26.33175861319207	23.589556062723226
20	26.18499337826886	24.141949845138157	25.167101780036887	24.505954996556092
21	25.6911764482281	24.378690037601704	25.464319436567468	24.465814077602737
22	25.746902383062963	24.580534999384202	25.720483880532107	23.952078737020724
23	25.1542536449931	24.42122572728807	25.23145649195737	25.193064135761457
24	24.697270569560107	24.97882718574146	25.93320034119781	24.390701903500624
25	25.16261633644172	25.039912845550212	25.114036701572033	24.683434116436036
26	24.98338865380434	25.21640364734986	25.386204295990623	24.414003402855176
27	25.23430740949667	24.130242077110097	25.543346870756885	25.09210364263635
28	24.645459894812547	24.88387262556581	25.4758751556601	24.994792323961544
29	24.78314020584385	25.346215425972694	25.876524100516512	23.994120267666947
30	25.084501195864878	25.000494159040144	24.98768403623022	24.927320608864754
31	24.150844707860777	25.86648887077817	25.70330235082859	24.27936407053246
32	24.104545807022532	24.936557581692092	25.47937228117497	25.479524330110404
33	23.598184839808845	25.568815067441303	25.883252265909263	24.94974782684059
34	25.544183139901744	24.056308282257564	25.58181525142052	24.817693326420176
35	24.454676493082534	24.49295481257688	25.842389114512613	25.209979579827973
36	23.86005111885209	25.5778239668655	26.003142851495326	24.558982062787088
37	24.655875246889458	24.22283987878659	26.425382745182706	24.695902129141242
38	24.818795681202037	24.719089591794223	25.704708803481314	24.757405923522427
39	24.435328266049144	24.452813893623524	26.17081481504007	24.941043025287257
40	23.633004046022172	24.18589198747725	26.708383826250643	25.472720140249937
41	22.795062362870866	24.620675918337557	27.21463075676276	25.369630962028822
42	23.523414775811446	24.360786275454892	27.607829303783127	24.50796964495053
43	24.01753580372307	24.028825437178703	27.05497937456191	24.89865938453632
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	213.0
1	243.5
2	274.0
3	798.0
4	1322.0
5	1322.0
6	1722.5
7	2123.0
8	1940.5
9	1758.0
10	2566.5
11	3375.0
12	3375.0
13	5759.0
14	8143.0
15	10956.0
16	13769.0
17	14294.0
18	14819.0
19	14819.0
20	18203.0
21	21587.0
22	22770.5
23	23954.0
24	28711.0
25	33468.0
26	33468.0
27	39142.0
28	44816.0
29	51761.5
30	58707.0
31	67044.0
32	75381.0
33	75381.0
34	84707.0
35	94033.0
36	103127.0
37	112221.0
38	120133.5
39	128046.0
40	128046.0
41	133101.0
42	138156.0
43	140470.5
44	142785.0
45	147434.0
46	152083.0
47	152083.0
48	161142.5
49	170202.0
50	172347.5
51	174493.0
52	175469.0
53	176445.0
54	176445.0
55	172449.0
56	168453.0
57	166439.0
58	164425.0
59	169129.5
60	173834.0
61	173834.0
62	160334.5
63	146835.0
64	135674.0
65	124513.0
66	108083.5
67	91654.0
68	91654.0
69	81422.0
70	71190.0
71	60243.0
72	49296.0
73	36257.5
74	23219.0
75	23219.0
76	16758.5
77	10298.0
78	8196.5
79	6095.0
80	4966.5
81	3838.0
82	3838.0
83	2981.0
84	2124.0
85	1746.5
86	1369.0
87	1138.5
88	908.0
89	908.0
90	678.5
91	449.0
92	243.5
93	38.0
94	23.0
95	8.0
96	8.0
97	10.0
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2630732.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.06370144914008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6491642089439	48.87458335787548
2	9.192338130360044	10.49097677380824
3	2.28606124568027	3.9135334925384466
4	0.8687509472625702	1.9829657875299576
5	0.4557600413491344	1.3003677465997372
6	0.2919846870099259	0.9997036204353013
7	0.1952809506771545	0.7800417707701776
8	0.13342489012362174	0.6090974476718936
9	0.10980544882048314	0.5639314814082792
>10	0.6472093272573703	7.204186925330179
>50	0.07910030352720959	3.177383708548636
>100	0.07457464928317468	9.239771762571788
>500	0.010807731823610734	4.365452797176263
>1k	0.005470580305776558	5.657881130752516
>5k	2.668575758915394E-4	0.840122196983202
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	5956	0.22640086485434474	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5664	0.21530129256800007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5421	0.20606431974066536	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	5027	0.19108749960087154	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4938	0.18770441078756786	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4839	0.18394119963569075	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	4743	0.18029202518538567	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3674	0.13965694719188423	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	3468	0.13182642701727124	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	3407	0.12950768075197322	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3247	0.12342572333479808	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	3160	0.12011865898920908	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	3083	0.11719171698219355	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	2894	0.11000740478315542	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	2831	0.1076126340501427	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	2820	0.1071944994777119	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	2679	0.1018347745038263	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	2671	0.10153067663296755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.520489354293786E-4	0.0
2	0.0	0.0	0.0	9.503058464336162E-4	0.0
3	0.0	0.0	0.0	0.0017865749912951985	0.0
4	0.0	0.0	0.0	0.0032690521117316397	0.0
5	0.0	0.0	0.0	0.003687186684162431	0.0
6	3.801223385734465E-5	0.0	0.0	0.0037251989180197755	0.0
7	3.801223385734465E-5	0.0	0.0	0.004181345724307911	0.0
8	3.801223385734465E-5	0.0	0.0	0.004561468062881358	0.0
9	3.801223385734465E-5	0.0	0.0	0.007336361134467517	0.0
10	3.801223385734465E-5	0.0	0.0	0.01079547441548588	0.0
11	3.801223385734465E-5	0.0	0.0	0.01235397600363701	0.0
12	3.801223385734465E-5	0.0	0.0	0.013456330785500006	0.0
13	3.801223385734465E-5	0.0	0.0	0.014368624398076277	0.0
14	3.801223385734465E-5	0.0	0.0	0.015166881309080515	0.0
15	3.801223385734465E-5	0.0	0.0	0.016877431832661025	0.0
16	3.801223385734465E-5	0.0	0.0	0.01854997012238419	0.0
17	3.801223385734465E-5	0.0	0.0	0.02079269191996752	0.0
18	3.801223385734465E-5	0.0	0.0	0.021971071169545206	0.0
19	3.801223385734465E-5	0.0	0.0	0.02379565839469775	0.0
20	1.1403670157203394E-4	0.0	0.0	0.02600036795842374	0.0
21	1.1403670157203394E-4	0.0	0.0	0.029421469005584757	0.0
22	1.1403670157203394E-4	0.0	0.0	0.03443908387475425	0.0
23	1.1403670157203394E-4	0.0	0.0	0.041053212565932216	0.0
24	1.1403670157203394E-4	0.0	0.0	0.050328197627124316	0.0
25	1.1403670157203394E-4	0.0	0.0	0.05333116410185454	0.0
26	1.1403670157203394E-4	0.0	0.0	0.057550522060019794	0.0
27	1.1403670157203394E-4	0.0	0.0	0.06541905446849014	0.0
28	1.1403670157203394E-4	0.0	0.0	0.08031985014056923	0.0
29	1.1403670157203394E-4	0.0	0.0	0.1076126340501427	0.0
30	1.1403670157203394E-4	0.0	0.0	0.14684125939092238	0.0
31	1.1403670157203394E-4	0.0	0.0	0.3053142623421922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2935	0.0	18.531515	1
GCTTTAT	1465	0.0	18.31058	1
AAGACGG	385	0.0	18.25974	5
GACGGAC	355	0.0	18.239437	7
ACGGACC	375	0.0	17.266666	8
CAAGACG	530	0.0	16.40566	4
AGACGGA	460	0.0	16.086956	6
CGGACCA	420	0.0	15.416666	9
TATTGAG	1645	0.0	15.407295	5
CGAATTA	145	5.3536496E-8	15.310345	15
GAATCGT	75	0.0041062767	14.8	6
CGCAAGA	520	0.0	14.230769	2
TATACTG	405	0.0	14.160494	5
ACGCTAT	170	2.440538E-8	14.147059	10
CTTATAC	1265	0.0	14.039526	37
GAGCGAA	465	0.0	13.924731	16
GCGAAAG	480	0.0	13.875001	18
TTTATTG	1920	0.0	13.875001	3
TAATACT	440	0.0	13.875	4
ACCTAGG	1820	0.0	13.824175	1
>>END_MODULE