FastQCFastQC Report
Fri 10 Feb 2017
ERR1630689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140588
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32790.1531822097479758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.13449575537188846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24090.11253917147998588No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG22940.10716681584686076No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22390.10459742837014878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.018.656781
ACCGGTC650.001580536117.0769238
TAGCGTA1659.749783E-1015.6969697
TAGACTG2300.015.2826095
CGTATAT1701.4879333E-915.23529410
AGCGTAT1954.1836756E-1115.1794878
CAATACT2450.015.102044
ATAGCGT1853.0559022E-1015.06
GACGGAC3700.015.07
GCGTTAT3050.014.5573771
CGTTATT3050.014.5573772
TCTAGCG2950.014.42372928
CCAATAC2700.014.3888893
AAGACGG3950.014.0506325
CGCAAGA3850.013.9350662
ACGGACC3650.013.6849328
AGCGAAA3800.013.63157917
TACAGCG950.001245687513.6315795
CGGACCA3950.013.5822789
GCGAACT1501.3068893E-613.56666728
Produced by FastQC (version 0.11.5)