FastQCFastQC Report
Fri 10 Feb 2017
ERR1630688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015204
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89240.4428335791314427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76770.3809539877848595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75180.37306396771741224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53350.26473746578510166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG2350.016.5319165
CGAATTA1601.0977601E-815.03125115
GTATTAG4600.014.8804341
GGTATCA49050.014.4454641
TTACACT2059.276846E-1114.4390244
CTACTAG1203.3027827E-513.8750011
AAGACGG3400.013.60294155
CGCAATA1501.3067802E-613.56666636
CCTATAC1652.5976624E-713.4545453
CTTATAC12150.013.39917837
ATAGTAC2355.638867E-1113.3829793
CTATACA1951.0270014E-813.2820524
TAACACG1000.001833313312.9500014
GTAAACG2305.7661964E-1012.86956427
AGCGTAT1307.0029164E-512.8076938
AGTCGGT4200.012.77380911
CGGTGAT4350.012.75862114
TCTTATA18500.012.69999937
CAAGACA3500.012.6857144
TAATACT4250.012.6235294
Produced by FastQC (version 0.11.5)