##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630688.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2015204
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14225061085627	33.0	31.0	34.0	30.0	34.0
2	32.310014271507995	34.0	31.0	34.0	30.0	34.0
3	32.41464635838357	34.0	31.0	34.0	30.0	34.0
4	35.96949440354425	37.0	35.0	37.0	35.0	37.0
5	35.91854373056028	37.0	35.0	37.0	35.0	37.0
6	35.99594879724336	37.0	35.0	37.0	35.0	37.0
7	35.94543182724925	37.0	35.0	37.0	35.0	37.0
8	35.9471065956598	37.0	35.0	37.0	35.0	37.0
9	37.660504842189674	39.0	37.0	39.0	35.0	39.0
10	37.58252067780731	39.0	37.0	39.0	35.0	39.0
11	37.67359532831416	39.0	37.0	39.0	35.0	39.0
12	37.62493375360509	39.0	37.0	39.0	35.0	39.0
13	37.65614051976872	39.0	37.0	39.0	35.0	39.0
14	38.981340846882006	40.0	38.0	41.0	36.0	41.0
15	38.98774069523483	40.0	38.0	41.0	36.0	41.0
16	38.94807771322407	40.0	38.0	41.0	36.0	41.0
17	38.94214283020478	40.0	38.0	41.0	36.0	41.0
18	38.929103455531056	40.0	38.0	41.0	35.0	41.0
19	38.99256303580184	40.0	39.0	41.0	35.0	41.0
20	38.95717356654711	40.0	38.0	41.0	35.0	41.0
21	38.91953916328074	40.0	38.0	41.0	35.0	41.0
22	38.846832380245374	40.0	38.0	41.0	35.0	41.0
23	38.79529070009786	40.0	38.0	41.0	35.0	41.0
24	38.74678444465176	40.0	38.0	41.0	35.0	41.0
25	38.69753880996663	40.0	38.0	41.0	35.0	41.0
26	38.55866006617692	40.0	38.0	41.0	34.0	41.0
27	38.430794103227264	40.0	38.0	41.0	34.0	41.0
28	38.33821985267993	40.0	38.0	41.0	34.0	41.0
29	38.264999473998664	40.0	38.0	41.0	34.0	41.0
30	38.1719294920018	40.0	38.0	41.0	34.0	41.0
31	38.11910258217034	40.0	38.0	41.0	33.0	41.0
32	38.014037784760255	40.0	37.0	41.0	33.0	41.0
33	37.93056831963414	40.0	37.0	41.0	33.0	41.0
34	37.8574779526043	40.0	37.0	41.0	33.0	41.0
35	37.78489473026056	40.0	37.0	41.0	33.0	41.0
36	37.73733378853952	40.0	37.0	41.0	33.0	41.0
37	37.59281045492168	40.0	37.0	41.0	33.0	41.0
38	37.43485274939907	40.0	37.0	41.0	32.0	41.0
39	37.35488069694185	40.0	36.0	41.0	32.0	41.0
40	37.17129432057499	40.0	36.0	41.0	31.0	41.0
41	37.085317913223676	40.0	36.0	41.0	31.0	41.0
42	36.94107197087739	40.0	36.0	41.0	31.0	41.0
43	36.14965085420632	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	11.0
11	11.0
12	15.0
13	5.0
14	1.0
15	3.0
16	9.0
17	26.0
18	57.0
19	102.0
20	265.0
21	468.0
22	917.0
23	1629.0
24	2509.0
25	4291.0
26	6724.0
27	9967.0
28	14153.0
29	19877.0
30	27101.0
31	35184.0
32	45717.0
33	58902.0
34	78251.0
35	105063.0
36	147964.0
37	235965.0
38	435188.0
39	784819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.852779172728916	18.509887832695846	11.679760460975663	24.957572533599574
2	18.808616894369006	20.78424814559717	34.47010823718095	25.937026722852874
3	21.183562557438353	23.715564280340846	28.025549770643565	27.07532339157723
4	14.530489220942396	15.02830482670737	33.78084799355301	36.66035795879722
5	14.337158917906079	37.39819889202284	33.81652676354354	14.448115426527538
6	35.35121010081361	33.61545530874294	15.797159989757862	15.236174600685587
7	29.506690141543984	29.984904754059638	21.5108247105504	18.997580393845983
8	28.083955768249762	32.46787918245498	20.016335815133356	19.431829234161903
9	27.34135104932305	13.48612845151161	19.79055222200829	39.38196827715706
10	17.481257480632234	26.69630469173344	32.403071847812924	23.419365979821396
11	35.073769206492244	21.937779004011503	21.235964200150455	21.752487589345794
12	22.340170027451315	25.339767090577432	28.922878279320607	23.39718460265065
13	30.125287563938937	19.948054886750917	25.681270978025054	24.245386571285092
14	23.41663672759681	20.67612013473574	25.762354580479197	30.144888557188253
15	26.13958686068507	26.852715655586234	23.037171422843546	23.97052606088515
16	25.272577863084827	25.98625250843091	24.685342029888783	24.055827598595478
17	23.62837707745717	26.27257587817412	25.911074015335423	24.187973029033287
18	23.44576529224833	24.87207250481837	27.548377236250026	24.133784966683276
19	24.142667442105118	25.126885417059512	27.510018836802626	23.22042830403274
20	24.707870766433572	24.33932247057866	27.42203766963543	23.530769093352337
21	24.914351102915635	24.678891070085214	26.653430620423542	23.753327206575612
22	24.825824085303523	24.640383802334654	26.72096720729018	23.812824905071643
23	24.262903408290178	24.918767529242697	26.917820726834602	23.900508335632523
24	23.706830673222164	25.53289890254287	26.88239007068267	23.877880353552293
25	24.336245858980035	25.139985827737537	26.715558325608725	23.808209987673703
26	23.94913864799792	25.77178290634596	26.73540743269664	23.543671012959482
27	24.449534637684323	25.064410352500293	26.281210239757364	24.204844770058017
28	23.697154233516805	25.242159106472595	27.0777549071955	23.9829317528151
29	23.34637088850558	25.577956375632443	27.422633142848067	23.653039593013908
30	23.356642801423575	26.042028499347957	27.108223286575456	23.493105412653012
31	23.627434244870493	26.007937657924458	26.62013374328356	23.74449435392149
32	23.00665342069587	25.77624895544074	26.975532005692727	24.241565618170664
33	23.108280848986006	25.74677303141518	27.152337927078353	23.99260819252046
34	24.053644196815807	25.243796657807348	26.89563934966386	23.80691979571299
35	23.629270287276128	25.563665018529143	27.281307500382095	23.525757193812634
36	23.239533069604864	25.969331144638456	26.794805885657237	23.996329900099443
37	23.764641197615724	25.124354655905805	27.066540161690828	24.044463984787644
38	23.377335495562733	25.115720294322557	27.307855681112187	24.199088529002523
39	23.349546745639646	25.272280126478506	27.276146732539235	24.102026395342605
40	22.74137010446585	25.21466809315583	27.767709869571515	24.2762519328068
41	22.20112703230045	25.187623684748537	28.279965700742952	24.331283582208055
42	22.759234300844977	25.196903142312145	28.035176587581205	24.008685969261673
43	22.509979138588452	24.721268913717918	28.098743353030265	24.67000859466337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	202.0
1	240.5
2	279.0
3	887.5
4	1496.0
5	1496.0
6	2162.5
7	2829.0
8	2822.5
9	2816.0
10	4251.5
11	5687.0
12	5687.0
13	9497.5
14	13308.0
15	17398.5
16	21489.0
17	21120.5
18	20752.0
19	20752.0
20	23394.5
21	26037.0
22	24029.0
23	22021.0
24	24120.0
25	26219.0
26	26219.0
27	29924.5
28	33630.0
29	38920.0
30	44210.0
31	51608.5
32	59007.0
33	59007.0
34	65352.5
35	71698.0
36	78744.5
37	85791.0
38	93262.0
39	100733.0
40	100733.0
41	104768.0
42	108803.0
43	112543.5
44	116284.0
45	120890.5
46	125497.0
47	125497.0
48	129069.0
49	132641.0
50	136187.0
51	139733.0
52	141686.0
53	143639.0
54	143639.0
55	138744.0
56	133849.0
57	128492.0
58	123135.0
59	115358.5
60	107582.0
61	107582.0
62	101302.0
63	95022.0
64	85216.5
65	75411.0
66	66539.0
67	57667.0
68	57667.0
69	50325.0
70	42983.0
71	37295.0
72	31607.0
73	24829.5
74	18052.0
75	18052.0
76	14166.0
77	10280.0
78	8445.0
79	6610.0
80	5237.5
81	3865.0
82	3865.0
83	2909.5
84	1954.0
85	1565.0
86	1176.0
87	962.5
88	749.0
89	749.0
90	572.5
91	396.0
92	222.0
93	48.0
94	31.5
95	15.0
96	15.0
97	8.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2015204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.3640851357239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15477740637868	49.69980678259503
2	8.809200312759712	10.282818340631069
3	2.413997704967825	4.226723027105527
4	1.0371938217333179	2.4213947405556078
5	0.5768004535675001	1.6832215389168868
6	0.37221708918943436	1.3034465929454087
7	0.2516332678062025	1.0280441825655129
8	0.18576123759089577	0.8673427748537978
9	0.14416646590690918	0.7572729500916555
>10	0.8590132313202801	9.684095780736898
>50	0.10347972606485471	4.224557390437083
>100	0.0852853193920598	9.716316841655468
>500	0.00528139113137845	2.0300821535335976
>1k	8.518372792545883E-4	0.610521118835074
>5k	3.4073491170183537E-4	1.46435578454139
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8924	0.4428335791314427	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7677	0.3809539877848595	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7518	0.37306396771741224	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5335	0.26473746578510166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.924553543958825E-5	0.0
2	0.0	0.0	0.0	0.0011413236575552649	0.0
3	0.0	0.0	0.0	0.0016375513347532062	0.0
4	0.0	0.0	0.0	0.0032254799017866183	0.0
5	0.0	0.0	0.0	0.0037217075789845594	0.0
6	0.0	0.0	0.0	0.0038209531144241474	0.0
7	0.0	0.0	0.0	0.0045652946302210595	0.0
8	0.0	0.0	0.0	0.0049126540042596185	0.0
9	0.0	4.962276771979412E-5	0.0	0.008535116047804589	0.0
10	0.0	4.962276771979412E-5	0.0	0.012951542374866266	0.0
11	4.962276771979412E-5	9.924553543958825E-5	0.0	0.014241734335580914	0.0
12	4.962276771979412E-5	9.924553543958825E-5	0.0	0.01578004013489453	0.0
13	4.962276771979412E-5	9.924553543958825E-5	0.0	0.016524381650691443	0.0
14	4.962276771979412E-5	9.924553543958825E-5	0.0	0.01756645977280712	0.0
15	4.962276771979412E-5	9.924553543958825E-5	0.0	0.01984910708791765	0.0
16	4.962276771979412E-5	9.924553543958825E-5	0.0	0.024612892789017887	0.0
17	4.962276771979412E-5	9.924553543958825E-5	0.0	0.029922528935035858	0.0
18	4.962276771979412E-5	9.924553543958825E-5	0.0	0.03270140392734433	0.0
19	4.962276771979412E-5	1.4886830315938236E-4	0.0	0.036968961951246625	0.0
20	4.962276771979412E-5	1.4886830315938236E-4	0.0	0.040492178459352005	0.0
21	4.962276771979412E-5	1.4886830315938236E-4	0.0	0.04773710254644195	0.0
22	4.962276771979412E-5	1.4886830315938236E-4	0.0	0.058157883767598714	0.0
23	4.962276771979412E-5	1.984910708791765E-4	0.0	0.07522811586320789	0.0
24	4.962276771979412E-5	1.984910708791765E-4	0.0	0.0996921403490664	0.0
25	4.962276771979412E-5	1.984910708791765E-4	0.0	0.1063912139912386	0.0
26	4.962276771979412E-5	1.984910708791765E-4	0.0	0.11755633672819228	4.962276771979412E-5
27	4.962276771979412E-5	1.984910708791765E-4	0.0	0.13209580767009196	4.962276771979412E-5
28	4.962276771979412E-5	1.984910708791765E-4	0.0	0.15799889241982448	4.962276771979412E-5
29	4.962276771979412E-5	1.984910708791765E-4	0.0	0.2018157963164027	4.962276771979412E-5
30	4.962276771979412E-5	1.984910708791765E-4	0.0	0.26205783632823276	4.962276771979412E-5
31	4.962276771979412E-5	1.984910708791765E-4	0.0	0.4832265120553552	4.962276771979412E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAG	235	0.0	16.531916	5
CGAATTA	160	1.0977601E-8	15.031251	15
GTATTAG	460	0.0	14.880434	1
GGTATCA	4905	0.0	14.445464	1
TTACACT	205	9.276846E-11	14.439024	4
CTACTAG	120	3.3027827E-5	13.875001	1
AAGACGG	340	0.0	13.6029415	5
CGCAATA	150	1.3067802E-6	13.566666	36
CCTATAC	165	2.5976624E-7	13.454545	3
CTTATAC	1215	0.0	13.399178	37
ATAGTAC	235	5.638867E-11	13.382979	3
CTATACA	195	1.0270014E-8	13.282052	4
TAACACG	100	0.0018333133	12.950001	4
GTAAACG	230	5.7661964E-10	12.869564	27
AGCGTAT	130	7.0029164E-5	12.807693	8
AGTCGGT	420	0.0	12.773809	11
CGGTGAT	435	0.0	12.758621	14
TCTTATA	1850	0.0	12.699999	37
CAAGACA	350	0.0	12.685714	4
TAATACT	425	0.0	12.623529	4
>>END_MODULE