FastQCFastQC Report
Fri 10 Feb 2017
ERR1630686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644984
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8300.12868536273768033No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG7280.11287101695545937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTG551.9009809E-523.5454545
GATTATA806.9503767E-720.81251
GTGTTAT450.003824014420.5555571
GACGGAC656.896127E-519.9230777
GTATTAG1254.110916E-1019.241
TTAGGAC2250.018.9111123
ATATGCC801.6148777E-518.535
GAGCGAA701.2181446E-418.516
TATTAGA500.007031616318.52
TATACTG609.2308264E-418.55
TCGCCAT1800.017.47222313
TTTCGTC852.7201166E-517.41176417
GATATGC852.7201166E-517.41176434
GCGAAAG752.0654943E-417.26666618
TAGAACA3000.017.2666664
ACGGACC752.0654943E-417.2666668
GACCGTG650.001578917417.0769237
AATTAGA650.001578917417.0769232
CAAGACG1107.7945333E-716.8181824
TAGGACA2650.016.7547174
Produced by FastQC (version 0.11.5)