FastQCFastQC Report
Fri 10 Feb 2017
ERR1630684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500929
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17820.11872646874036014No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17470.11639457962368639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC3200.023.1253
GTCTAGA3050.021.229511
TAGAACT4250.020.8941154
GCAATCG555.1440997E-420.18181825
ACTGATC4350.019.137938
GGTATCA10400.019.0336531
AATCTAC3500.019.02857214
GTTCTAG2200.018.51
TAACGCT500.00703585918.54
GGTATAT500.00703585918.51
TTAGTAC500.00703585918.53
TCTAGAC2050.018.048783
ACAACGG3700.018.023
TACAACG3700.017.522
GAATCTA3850.017.298713
CCGTATG650.001580238817.0769239
CGCAATA1851.8189894E-1217.036
ACGGAGT3900.016.60256426
CTAGACA3350.016.5671634
GGTATAG4400.016.3977281
Produced by FastQC (version 0.11.5)