FastQCFastQC Report
Fri 10 Feb 2017
ERR1630683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261857
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58720.46534591479066173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58150.46082876268864065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48450.38395792867179085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34230.2712668709687389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGT400.001931291123.1250028
ATACCGT1600.019.656256
TACCGTC1551.8189894E-1219.0967737
GGTATCA28250.018.401771
TCGGTGA1451.546141E-1017.8620713
CGGTGAT1452.9795046E-916.58620814
CCAATAC1352.2197128E-816.4444453
AGTCGGT1606.2937033E-1016.187511
ACACCGT700.002592681715.8571436
GTAATAC1403.473542E-815.8571433
GTCGTAG1751.3096724E-1015.85714311
CGTCGTA1751.3096724E-1015.85714310
ACCGTCG1751.3096724E-1015.8571438
GTCCTAC1557.208655E-915.5161291
CGGACAT2750.015.47272721
CGAACTA855.3646695E-415.23529329
GCGCAAG1701.4861143E-915.2352931
TGCGGTA1101.4520132E-515.13636423
TCTATGC1101.4520132E-515.1363643
CATATAC1601.0964868E-815.0312513
Produced by FastQC (version 0.11.5)