##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630683.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1261857
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.059112878876135	33.0	31.0	34.0	30.0	34.0
2	32.23833524717936	33.0	31.0	34.0	30.0	34.0
3	32.33403626559903	34.0	31.0	34.0	30.0	34.0
4	35.91148759328514	37.0	35.0	37.0	35.0	37.0
5	35.870431435574716	37.0	35.0	37.0	35.0	37.0
6	35.9472206438606	37.0	35.0	37.0	35.0	37.0
7	35.90099115826912	37.0	35.0	37.0	35.0	37.0
8	35.90413652260121	37.0	35.0	37.0	35.0	37.0
9	37.60774636111699	39.0	37.0	39.0	35.0	39.0
10	37.52118346215142	39.0	37.0	39.0	35.0	39.0
11	37.61346491718158	39.0	37.0	39.0	35.0	39.0
12	37.570052708032684	39.0	37.0	39.0	35.0	39.0
13	37.6036024684255	39.0	37.0	39.0	35.0	39.0
14	38.901543518798086	40.0	38.0	41.0	36.0	41.0
15	38.913930025351526	40.0	38.0	41.0	36.0	41.0
16	38.87774842949716	40.0	38.0	41.0	35.0	41.0
17	38.871462455729926	40.0	38.0	41.0	35.0	41.0
18	38.86107300589528	40.0	38.0	41.0	35.0	41.0
19	38.93033838224141	40.0	38.0	41.0	35.0	41.0
20	38.90194689255597	40.0	38.0	41.0	35.0	41.0
21	38.864162896429626	40.0	38.0	41.0	35.0	41.0
22	38.79344489906543	40.0	38.0	41.0	35.0	41.0
23	38.73713423945819	40.0	38.0	41.0	35.0	41.0
24	38.68799951183058	40.0	38.0	41.0	34.0	41.0
25	38.63395059820566	40.0	38.0	41.0	34.0	41.0
26	38.501602796513396	40.0	38.0	41.0	34.0	41.0
27	38.376071139598224	40.0	38.0	41.0	34.0	41.0
28	38.286245588842476	40.0	38.0	41.0	34.0	41.0
29	38.212663558549025	40.0	38.0	41.0	34.0	41.0
30	38.11466671738557	40.0	38.0	41.0	33.0	41.0
31	38.059358548551856	40.0	37.0	41.0	33.0	41.0
32	37.95280447784496	40.0	37.0	41.0	33.0	41.0
33	37.873392943891425	40.0	37.0	41.0	33.0	41.0
34	37.80609371743391	40.0	37.0	41.0	33.0	41.0
35	37.741932722963064	40.0	37.0	41.0	33.0	41.0
36	37.70245756848835	40.0	37.0	41.0	33.0	41.0
37	37.565213807903746	40.0	37.0	41.0	33.0	41.0
38	37.40985705987287	40.0	37.0	41.0	32.0	41.0
39	37.33730208732051	40.0	36.0	41.0	32.0	41.0
40	37.16125361273108	40.0	36.0	41.0	31.0	41.0
41	37.0876898095426	40.0	36.0	41.0	31.0	41.0
42	36.95078523160707	40.0	36.0	41.0	31.0	41.0
43	36.18076453988051	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	4.0
12	4.0
13	2.0
14	2.0
15	3.0
16	7.0
17	8.0
18	31.0
19	76.0
20	153.0
21	300.0
22	572.0
23	985.0
24	1563.0
25	2624.0
26	4194.0
27	6131.0
28	9089.0
29	12675.0
30	17303.0
31	22754.0
32	29538.0
33	38592.0
34	50186.0
35	68266.0
36	95324.0
37	150440.0
38	277656.0
39	473370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3709207937191	19.883077084011898	12.532957379481195	24.21304474278781
2	18.117504598381593	21.80183649969846	35.694456661888	24.386202240031952
3	20.390345340240614	24.372809280290873	29.91075850908621	25.3260868703823
4	14.298292120264023	15.968053432361987	34.89492073983026	34.838733707543724
5	13.63292354046457	38.05526299731269	34.39652829124061	13.91528517098213
6	33.60602667338692	35.154538113272736	16.309138040205823	14.930297173134516
7	27.800535242899947	31.553258412007068	22.065020045853057	18.58118629923993
8	26.69296124679738	33.84805092811626	20.81567087237302	18.64331695271334
9	26.530264522842128	14.100409158882504	20.928044936946105	38.44128138132926
10	16.7583173053682	27.662088493387127	33.563074104276474	22.016520096968197
11	33.91549121651661	22.586711489495244	22.271382573461178	21.22641472052697
12	20.941517144969676	26.688761087825323	29.998407109521917	22.37131465768308
13	29.24340872222447	20.790866159953147	26.44800480561585	23.51772031220653
14	22.368144726383417	22.07849225387663	26.695021702142157	28.858341317597798
15	24.94545736957516	28.281730814188926	24.008742670524473	22.764069145711442
16	23.957627528317392	27.4029466096396	25.406127635698816	23.23329822634419
17	22.321705232843343	27.52300775761437	26.967001807653325	23.188285201888963
18	21.942502201121048	26.060480704231935	28.719102085260058	23.27791500938696
19	22.719927852363618	26.541200785825968	28.777983559151316	21.960887802659094
20	23.532222747902495	25.619464012166198	28.699131518072175	22.149181721859133
21	23.718614708322733	26.11952067468818	27.586644128455124	22.57522048853396
22	23.517244822511586	26.063888380379076	27.744189714048424	22.674677083060917
23	22.88365480399126	26.322792519279126	27.905697713766298	22.887854962963317
24	23.05475184589062	26.672911431327005	27.873364414509727	22.39897230827265
25	23.08399446213002	26.283881612575748	27.911799831518152	22.720324093776078
26	22.798145907182825	26.715626255589974	27.876772090656864	22.60945574657033
27	23.081775510220254	26.323822746951514	27.574202148103943	23.020199594724282
28	22.5890096896875	26.48818368483909	28.27927411743169	22.64353250804172
29	22.160197233125466	26.96486210402605	28.290289628698023	22.584651034150465
30	22.207429209490456	27.48457234060595	28.185919640656586	22.122078809247007
31	22.613259664129927	27.17344358354394	27.677858901602953	22.53543785072318
32	21.844234330831465	27.00741843172404	28.074338058908417	23.074009178536077
33	22.025871394302207	26.661024188953263	28.41177724575764	22.90132717098689
34	22.530603705491192	26.353144611473407	28.22776273381215	22.888488949223248
35	22.313938901159165	26.530898509102062	28.54649932599336	22.608663263745417
36	22.00368187520456	26.779500371278203	28.21429052578858	23.002527227728656
37	22.55675563871342	25.909671222650427	28.23006093400441	23.303512204631748
38	21.976658210874923	25.99090071220431	28.98474232817189	23.047698748748868
39	21.97261654846785	25.905629560243355	29.02531744880759	23.0964364424812
40	21.37373727767885	25.698236804962843	29.745842833221197	23.182183084137108
41	20.980824293085508	25.899368945926522	29.969402238129994	23.15040452285798
42	21.39814574868626	25.65789942917462	29.71929465858651	23.224660163552606
43	20.791262401365607	25.504474754270888	29.807735741847136	23.89652710251637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	391.0
1	352.0
2	313.0
3	737.5
4	1162.0
5	1162.0
6	1636.0
7	2110.0
8	2099.0
9	2088.0
10	3063.5
11	4039.0
12	4039.0
13	6815.0
14	9591.0
15	12836.0
16	16081.0
17	15827.5
18	15574.0
19	15574.0
20	17475.0
21	19376.0
22	18817.0
23	18258.0
24	20446.0
25	22634.0
26	22634.0
27	25608.0
28	28582.0
29	34000.5
30	39419.0
31	43088.0
32	46757.0
33	46757.0
34	50470.0
35	54183.0
36	57668.5
37	61154.0
38	65920.0
39	70686.0
40	70686.0
41	73922.0
42	77158.0
43	76050.5
44	74943.0
45	76703.5
46	78464.0
47	78464.0
48	79928.5
49	81393.0
50	82074.0
51	82755.0
52	85302.0
53	87849.0
54	87849.0
55	83041.5
56	78234.0
57	73807.0
58	69380.0
59	63520.5
60	57661.0
61	57661.0
62	52754.0
63	47847.0
64	42042.0
65	36237.0
66	31317.0
67	26397.0
68	26397.0
69	22520.0
70	18643.0
71	15942.0
72	13241.0
73	10652.0
74	8063.0
75	8063.0
76	6306.0
77	4549.0
78	3770.5
79	2992.0
80	2316.0
81	1640.0
82	1640.0
83	1237.0
84	834.0
85	694.0
86	554.0
87	476.0
88	398.0
89	398.0
90	294.5
91	191.0
92	109.0
93	27.0
94	16.0
95	5.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1261857.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.49063319094866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63503512040384	58.93399608057624
2	7.386489449390416	9.822647210964607
3	1.7123631371169554	3.415683277192368
4	0.6286847681830422	1.67206593255981
5	0.34900281459538984	1.1602709063935364
6	0.21069506310683636	0.8405548893708262
7	0.14779177383598363	0.687873803593762
8	0.10965596634791334	0.5832875708510492
9	0.08391995368282106	0.502190177195327
>10	0.5711487151929516	7.634452116739013
>50	0.09691276535748619	4.595949618297853
>100	0.06579732340081876	7.7057667372592995
>500	0.0020263590263871058	0.8637235204942714
>1k	2.3839517957495362E-4	0.6552822598134185
>5k	2.3839517957495362E-4	0.9262558986985272
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5872	0.46534591479066173	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5815	0.46082876268864065	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4845	0.38395792867179085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3423	0.2712668709687389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.92482824915977E-5	0.0
2	0.0	7.92482824915977E-5	0.0	0.0013472208023571608	0.0
3	0.0	7.92482824915977E-5	0.0	0.0020604553447815402	0.0
4	0.0	7.92482824915977E-5	0.0	0.002852938169697517	0.0
5	0.0	1.584965649831954E-4	0.0	0.002932186452189115	0.0
6	0.0	1.584965649831954E-4	0.0	0.002932186452189115	0.0
7	0.0	1.584965649831954E-4	0.0	0.0033284278646471036	0.0
8	0.0	1.584965649831954E-4	0.0	0.0035661727121218964	0.0
9	0.0	1.584965649831954E-4	0.0	0.006181366034344621	0.0
10	0.0	1.584965649831954E-4	0.0	0.0103022767239077	0.0
11	0.0	1.584965649831954E-4	0.0	0.011887242373739655	0.0
12	0.0	1.584965649831954E-4	0.0	0.01347220802357161	0.0
13	0.0	2.377448474747931E-4	0.0	0.01418544256599599	0.0
14	0.0	2.377448474747931E-4	0.0	0.015611911650844747	0.0
15	0.0	2.377448474747931E-4	0.0	0.018147856690575875	0.0
16	0.0	2.377448474747931E-4	0.0	0.02131778799023978	0.0
17	0.0	2.377448474747931E-4	0.0	0.02591418837475245	0.0
18	0.0	2.377448474747931E-4	0.0	0.028053892002025585	0.0
19	0.0	2.377448474747931E-4	0.0	0.03058983704175671	0.0
20	0.0	2.377448474747931E-4	0.0	0.03288803723401305	0.0
21	0.0	2.377448474747931E-4	0.0	0.038435417008424885	0.0
22	0.0	2.377448474747931E-4	0.0	0.0481829557548914	0.0
23	0.0	3.169931299663908E-4	0.0	0.05991170156364786	0.0
24	0.0	3.169931299663908E-4	0.0	0.08083324814142966	0.0
25	0.0	3.169931299663908E-4	0.0	0.08693536589328268	0.0
26	0.0	3.169931299663908E-4	0.0	0.09715839433469878	0.0
27	0.0	3.169931299663908E-4	0.0	0.11498925789530827	0.0
28	0.0	4.754896949495862E-4	0.0	0.15302843349127515	0.0
29	0.0	5.547379774411839E-4	0.0	0.20873997608286834	0.0
30	0.0	5.547379774411839E-4	0.0	0.2982112870158822	0.0
31	0.0	5.547379774411839E-4	0.0	0.6108457614452351	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTCGT	40	0.0019312911	23.125002	8
ATACCGT	160	0.0	19.65625	6
TACCGTC	155	1.8189894E-12	19.096773	7
GGTATCA	2825	0.0	18.40177	1
TCGGTGA	145	1.546141E-10	17.86207	13
CGGTGAT	145	2.9795046E-9	16.586208	14
CCAATAC	135	2.2197128E-8	16.444445	3
AGTCGGT	160	6.2937033E-10	16.1875	11
ACACCGT	70	0.0025926817	15.857143	6
GTAATAC	140	3.473542E-8	15.857143	3
GTCGTAG	175	1.3096724E-10	15.857143	11
CGTCGTA	175	1.3096724E-10	15.857143	10
ACCGTCG	175	1.3096724E-10	15.857143	8
GTCCTAC	155	7.208655E-9	15.516129	1
CGGACAT	275	0.0	15.472727	21
CGAACTA	85	5.3646695E-4	15.235293	29
GCGCAAG	170	1.4861143E-9	15.235293	1
TGCGGTA	110	1.4520132E-5	15.136364	23
TCTATGC	110	1.4520132E-5	15.136364	3
CATATAC	160	1.0964868E-8	15.031251	3
>>END_MODULE