FastQCFastQC Report
Fri 10 Feb 2017
ERR1630682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375526
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG25820.6875689033515655No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT17310.46095343598046473No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC15450.4114229107971219No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA15270.40662963416647585No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG14870.3959779083205956No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC14320.3813317852825104No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG14030.3736092840442473No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC13770.36668566224442517No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG13500.359495747298456No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA12750.3395237613374307No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA12600.33552936414522566No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT11710.3118292741381422No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG11510.30650341121520214No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC11460.30517194548446713No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG11420.30410677289987914No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT11390.3033078934614381No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA10390.27667857884673763No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG9860.2625650421009464No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA9830.26176616266250535No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA9680.25777176547030034No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA9280.24712003962442014No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA9270.24685374647827313No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC9050.24099529726303906No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA8490.2260828810788068No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT8440.22475141534807178No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC8300.2210233113020137No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC8110.2159637415252206No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG8000.21303451691760356No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT7880.2098389991638395No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC7860.2093064128715455No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC7800.20770865399466348No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG7700.20504572253319345No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC7580.2018502047794294No Hit
GTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCAGGACGATG7400.1970569281487833No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG7310.19466028983346026No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG7230.19252994466428425No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC6800.18107933937996304No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC6800.18107933937996304No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC6760.18001416679537502No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC6690.178150114772346No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC6680.17788382162619898No Hit
GTCAAACTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCC6640.17681864904161096No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC6530.17388942443399394No Hit
CTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCCGAACCT6270.1669658026341718No Hit
GCTTTGCGTTCTCGGGGTGCCATAGCCGGGTTTGAGTTAGCAG6230.16590063004958377No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT6220.16563433690343676No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC6020.1603084739804967No Hit
GGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCG6020.1603084739804967No Hit
GATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTGGGTTC5940.15817812881132065No Hit
AACTGACGGAGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGC5900.15711295622673263No Hit
CTCTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCG5890.15684666308058562No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG5840.15551519734985061No Hit
CTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGGGAGCGCCG5820.15498261105755662No Hit
GATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCT5680.15125450701149853No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG5680.15125450701149853No Hit
CACTGGAGGCGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGA5590.1488578686961755No Hit
GCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGACAGATC5560.14805898925773447No Hit
CCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTCCTGGCCCTG5540.14752640296544048No Hit
GATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGG5380.1432657126270884No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG5300.14113536745791236No Hit
CTGGAGGCGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGC5290.14086907431176535No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA5280.14060278116561836No Hit
GCATCATTCTCCGTCTGGTTGGGTTCAGACAGCAGCTCTGCCA5270.14033648801947135No Hit
GAATGATGCCCTGGAACCTGAAGATCTGTCCCAGGCTGCTGAG5120.1363420908272663No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG5060.13474433195038427No Hit
CCGAGATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCT5050.13447803880423725No Hit
TATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTCTCTTC5050.13447803880423725No Hit
TCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAG4960.1320814004889142No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA4910.1307499347581792No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG4870.12968476217359118No Hit
GGATTAGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAG4850.12915217588129718No Hit
GCTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGAT4810.12808700329670913No Hit
ACACAGCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGC4790.12755441700441514No Hit
GGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCTGGTTG4760.12675553756597413No Hit
CTGAAGATCTGTCCCAGGCTGCTGAGCAGGATGAAATGAGGCT4750.12648924441982712No Hit
CCTCTGATCCCTCGCCCCCACACCCCATCTCTCTTCCCTAATC4680.1246251923967981No Hit
GGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCCGTCAG4630.12329372666606307No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC4630.12329372666606307No Hit
ACGCAAAGCTGGCTGCAAGAATTTCTTCTGGAAGACTTTCACA4620.12302743351991607No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA4550.12116338149688703No Hit
CTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAGAGATCTGC4540.12089708835074002No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG4520.12036450205844602No Hit
GGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAGGCAGC4520.12036450205844602No Hit
CCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCG4480.11929932947385799No Hit
GTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCAG4470.119033036327711No Hit
AACTCTAGGCGCGGATCAGCAGGCAGCAGCGATGGCTCCGAAC4440.11823415688926998No Hit
GCCAAGTACTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGA4410.11743527745082898No Hit
CAACCAGACGGAGAATGATGCCCTGGAACCTGAAGATCTGTCC4350.11583751857394695No Hit
CTGCAGAAGTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGG4330.11530493228165294No Hit
TTCTTAACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCC4220.11237570767403589No Hit
GAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGG4170.11104424194330086No Hit
GAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCC4120.10971277621256584No Hit
TCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGG4080.10864760362797783No Hit
CGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTCCTGGCCC4080.10864760362797783No Hit
GGCTATGGCACCCCGAGAACGCAAAGCTGGCTGCAAGAATTTC4010.10678355160494879No Hit
GATCTGTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGC3980.10598467216650778No Hit
CATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCC3910.10412062014347875No Hit
ATCATTCTCCGTCTGGTTGGGTTCAGACAGCAGCTCTGCCAAG3900.10385432699733174No Hit
GGCTTGAGCTGCAGAGATCTGCTAACTCAAACCCGGCTATGGC3890.10358803385118473No Hit
GGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAG3880.10332174070503772No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG3870.10305544755889073No Hit
CTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACAC3840.10225656812044971No Hit
GTACATGGGAAGCCGCTTTAGGAGCGAGGTTCGGAGCCATCGC3840.10225656812044971No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT3820.10172398182815572No Hit
CTGTTAGCTTTCTTAACTAGTATTGTCCATATCAGACCTCTGA3810.1014576886820087No Hit
GAGCTGCTGTCTGAACCCAACCAGACGGAGAATGATGCCCTGG3790.1009251023897147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAC250.00549168929.62
ATAGTGT250.00549168929.66
ATTCTAA250.00549168929.61
ATGCTAG358.8582333E-426.4285715
TGAATAG358.8582333E-426.4285719
CTAGAAC509.069818E-625.9000023
TTAATAT451.3208951E-424.6666683
CCGTCTT551.8982384E-523.54545437
GTCCTAC400.001928779823.1251
TCTAATA400.001928779823.1252
AATAGCC400.001928779823.12521
AGCTAGG400.001928779823.12534
TATTTAC1201.0913936E-1121.5833342
GATCGCG450.00382108320.5555578
GAATAGC450.00382108320.55555720
TAGTCCC450.00382108320.5555575
ATGCCGT555.134234E-420.18181834
CTCTAAT555.134234E-420.1818181
GGTATCA2650.019.5471691
CTCGTAT500.007026277518.529
Produced by FastQC (version 0.11.5)