FastQCFastQC Report
Fri 10 Feb 2017
ERR1630681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833436
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64130.34978041229691137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53990.2944744185234718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48740.2658396584336732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34000.18544416058155289No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG24900.13581057642590197No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT20820.11355727715611562No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG20050.10935751234294516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC1201.0421718E-716.95833436
TTAACGG1201.0421718E-716.95833435
GGTATCA31350.016.700161
CGAATTA1352.2215318E-816.44444515
TAGGACA8500.015.8882364
CGACGGT2300.015.2826077
GCTTAGG8250.015.2484841
TCAATAC855.366313E-415.2352943
TTAGGAC9000.014.83
CTTAGGA9200.014.2771732
GTAACAC1304.447149E-614.2307693
ACGGACC3150.014.0952398
GTTATAG1051.6564789E-414.0952391
ACGACGG2405.456968E-1213.8750016
ATAGCAC1601.7849561E-713.8753
TTCGTAT2800.013.87525
CGATCGC2151.9826984E-1013.76744219
ACGGTAT2151.9826984E-1013.7674429
TGTACCG1356.5715976E-613.7037045
GTGCGTA1356.5715976E-613.70370415
Produced by FastQC (version 0.11.5)