FastQCFastQC Report
Fri 10 Feb 2017
ERR1630680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123305
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112270.9994614107477487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110510.9837933597731694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87360.7777050756473086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.45686612273603333No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG28560.2542497362693124No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23350.20786874446388115No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC22570.2009249491456016No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG22460.1999456959596904No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21920.1951384530470353No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA19140.1703900543485518No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG18600.16558281143589673No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG18190.16193286774295496No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC17200.15311958906975398No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC17060.1518732668331397No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT16320.14528556358246425No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG16280.14492947151486016No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC16190.144128264362751No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.1427038960923347No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC15940.14190268894022548No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG15730.14003320558530408No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15720.13994418256840307No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT15540.1383417682641847No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG15430.1373625150782735No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG15100.13442475552053984No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT15090.1343357325036388No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC14370.1279260752867654No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14020.1248102696952297No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC13910.12383101650931848No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC13840.12320785539101135No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT13690.12187251013749605No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC13210.11759940532624709No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG13180.11733233627554404No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT13140.11697624420793995No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.11572992197132567No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT12960.11537382990372161No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12800.1139494616333053No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC12740.11341532353189916No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT12600.1121690012952849No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA12400.11038854095726451No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA12230.10887514966994717No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT12170.10834101156854103No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA12110.10780687346713493No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC11850.10549227502770842No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG11720.10433497580799515No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG11720.10433497580799515No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT11610.10335572262208394No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG11590.1031776765882819No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG11510.10246549245307374No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC11500.10237646943617273No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11490.1022874464192717No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.10202037736856864No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT11350.10104112418265743No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA11240.1000618709967462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT200.001841918337.011
CTGTGCG251.233608E-436.9999969
GTCGTCA303.5994273E-430.83333224
ACTGTGC303.5994273E-430.8333328
TCGTTCG250.005496291429.59999823
CGTCTCT250.005496291429.59999820
GCTCTAG250.005496291429.5999981
CGTATAA405.9400125E-527.7530
TCCCAAC659.391442E-825.61538333
TCGTATA451.3231859E-424.66666829
CGTCCCA701.9228355E-723.78571531
TACCCTT802.7257556E-823.1255
TCGTCCC753.7406062E-722.230
AATCGTC753.7406062E-722.228
CTTCTGT603.7261896E-521.58333237
AATTAGT705.1017505E-621.1428596
CGTTTGG1059.822543E-1021.14285728
GTATGTA1600.020.81251
GTATTGA450.003825754220.5555576
AACCACG450.003825754220.5555577
Produced by FastQC (version 0.11.5)