##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630680.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123305
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79432211198205	31.0	31.0	34.0	30.0	34.0
2	31.952461708974855	33.0	31.0	34.0	30.0	34.0
3	32.00918628511402	33.0	31.0	34.0	30.0	34.0
4	35.66291345627412	37.0	35.0	37.0	33.0	37.0
5	35.63669973871745	37.0	35.0	37.0	33.0	37.0
6	35.73950440886491	37.0	35.0	37.0	35.0	37.0
7	35.687432175588995	37.0	35.0	37.0	33.0	37.0
8	35.707080445649225	37.0	35.0	37.0	33.0	37.0
9	37.32949020969372	39.0	37.0	39.0	34.0	39.0
10	37.20592982315578	39.0	37.0	39.0	34.0	39.0
11	37.33208345017604	39.0	37.0	39.0	34.0	39.0
12	37.27712598092237	39.0	37.0	39.0	34.0	39.0
13	37.33400812780144	39.0	37.0	39.0	34.0	39.0
14	38.512520642212046	40.0	38.0	41.0	34.0	41.0
15	38.47970942887284	40.0	38.0	41.0	34.0	41.0
16	38.41075754136232	40.0	38.0	41.0	34.0	41.0
17	38.44111617058591	40.0	38.0	41.0	34.0	41.0
18	38.45865993652659	40.0	38.0	41.0	34.0	41.0
19	38.56499436929418	40.0	38.0	41.0	34.0	41.0
20	38.516512434289886	40.0	38.0	41.0	34.0	41.0
21	38.466372000480725	40.0	38.0	41.0	34.0	41.0
22	38.39386186298467	40.0	38.0	41.0	34.0	41.0
23	38.3113268435554	40.0	38.0	41.0	34.0	41.0
24	38.25755427065668	40.0	38.0	41.0	34.0	41.0
25	38.17246874179319	40.0	37.0	41.0	33.0	41.0
26	37.99031785668185	40.0	37.0	41.0	33.0	41.0
27	37.83209101713248	40.0	37.0	41.0	33.0	41.0
28	37.68360240540191	40.0	37.0	41.0	33.0	41.0
29	37.59824713679722	40.0	37.0	41.0	33.0	41.0
30	37.42092753081309	39.0	36.0	41.0	32.0	41.0
31	37.33209858408892	39.0	36.0	41.0	32.0	41.0
32	37.18667058367941	39.0	36.0	41.0	32.0	41.0
33	37.05192801598854	39.0	35.0	41.0	31.0	41.0
34	36.95009814787613	39.0	35.0	41.0	31.0	41.0
35	36.813586692839436	39.0	35.0	41.0	31.0	41.0
36	36.75441220327516	39.0	35.0	41.0	31.0	41.0
37	36.53694410689884	39.0	35.0	41.0	30.0	41.0
38	36.323274622653685	39.0	35.0	40.0	30.0	41.0
39	36.18534770164826	39.0	35.0	40.0	30.0	41.0
40	35.90984995170501	39.0	35.0	40.0	28.0	41.0
41	35.761228695679264	38.0	35.0	40.0	27.0	41.0
42	35.49725319481352	38.0	35.0	40.0	26.0	41.0
43	34.664761574104986	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	11.0
11	13.0
12	2.0
13	4.0
14	4.0
15	6.0
16	8.0
17	17.0
18	45.0
19	88.0
20	200.0
21	479.0
22	824.0
23	1501.0
24	2457.0
25	3861.0
26	5897.0
27	8583.0
28	11982.0
29	16294.0
30	21165.0
31	26673.0
32	33186.0
33	42602.0
34	55055.0
35	72123.0
36	101618.0
37	164125.0
38	279103.0
39	275376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.74403657065535	20.224961163708876	12.611089597215361	22.41991266842042
2	19.869403234206203	21.612117813060568	34.200061425881664	24.31841752685157
3	20.897084941311576	26.53072851985881	29.078656286582895	23.49353025224672
4	13.021841797196664	16.554720222913634	35.71576731163842	34.707670668251275
5	12.2479647112761	40.51330671545128	34.09305575956664	13.145672813705984
6	31.51913327190745	36.081651911101616	16.54893372681507	15.850281090175866
7	27.74437930927041	32.06039321466565	22.390535072843086	17.804692403220855
8	29.064768695946334	31.46465118556403	19.339805306662036	20.1307748118276
9	27.77224351356043	12.794566035048364	21.240980855600217	38.19220959579099
10	19.785098437200936	27.20009258393758	31.117995557751456	21.89681342111003
11	33.500340513039646	24.259306243629293	20.268315372939675	21.97203787039139
12	22.539025465033983	29.144444296072752	26.382772265769315	21.933757973123953
13	28.995330742763542	20.698741659656104	27.354992633345354	22.950934964235003
14	22.94452530701813	21.770133668059877	26.670494656393412	28.614846368528585
15	26.65901068721318	26.898215533626217	25.435834434993165	21.006939344167435
16	24.500469596414153	25.610853686220576	26.65384735223292	23.234829365132356
17	23.104054553304756	27.30861164153992	26.26214607786843	23.325187727286888
18	21.15489559825693	24.79522480537343	29.80588531164733	24.243994284722316
19	22.671847806250305	25.403875171925698	30.130196162217743	21.79408085960625
20	22.998206186209444	23.778849021414487	31.27156026190572	21.951384530470353
21	23.32919376304744	25.70708756749058	29.52172384169927	21.441994827762716
22	24.710296847249857	25.19093211549846	28.11257850717303	21.986192530078654
23	22.77422427568648	25.52005020898153	29.71223309786745	21.993492417464537
24	21.546240780553813	27.386862873395916	27.66230008768767	23.404596258362602
25	22.47216917934132	25.827624732374556	29.614129733242528	22.086076355041595
26	22.107619925131644	27.235612767681083	28.833219828986785	21.82354747820049
27	22.981024743947547	26.38214910465101	28.316886330960873	22.319939820440574
28	21.591019358055025	26.364255478253902	30.396731074819392	21.647994088871677
29	22.28112578507173	26.626339240010505	30.0303123372548	21.062222637662966
30	20.180271609224565	27.445707087567488	30.49162961083588	21.882391692372064
31	22.18061879899048	27.91263281121334	28.49270678934038	21.414041600455796
32	20.791236574216263	27.09317594063945	28.98909913158047	23.126488353563815
33	20.257187495827043	27.364874188221368	29.44641036940101	22.93152794655058
34	22.138956027080802	26.636220794886516	29.697010161977378	21.5278130160553
35	20.934385585393102	28.343148120946672	29.067617432487168	21.654848861173058
36	21.637934487961864	28.501965183098086	27.7084140104424	22.15168631849765
37	21.452944658841545	26.687409029604602	29.36548844703798	22.49415786451587
38	21.024654924530736	26.5910861253177	29.128420153030564	23.255838797120994
39	21.06587258135591	28.000231459843945	29.165809820128995	21.76808613867115
40	20.697940452503996	27.85841779392062	28.680456331984633	22.763185421590755
41	20.71334143442787	27.41944529758169	28.869897311950005	22.997315956040435
42	20.141190504805017	28.582709059427312	29.1926057482162	22.083494687551468
43	20.68013584912379	26.289565167073945	28.76921227983495	24.26108670396731
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	79.5
2	131.0
3	584.0
4	1037.0
5	1037.0
6	1714.5
7	2392.0
8	2618.0
9	2844.0
10	3936.0
11	5028.0
12	5028.0
13	8845.0
14	12662.0
15	18965.5
16	25269.0
17	22681.5
18	20094.0
19	20094.0
20	20821.5
21	21549.0
22	15460.0
23	9371.0
24	8911.0
25	8451.0
26	8451.0
27	9945.5
28	11440.0
29	14425.5
30	17411.0
31	22078.5
32	26746.0
33	26746.0
34	34878.5
35	43011.0
36	47383.0
37	51755.0
38	62483.5
39	73212.0
40	73212.0
41	77876.5
42	82541.0
43	89475.0
44	96409.0
45	93472.0
46	90535.0
47	90535.0
48	93444.5
49	96354.0
50	92747.0
51	89140.0
52	83015.0
53	76890.0
54	76890.0
55	68643.5
56	60397.0
57	50281.0
58	40165.0
59	38234.0
60	36303.0
61	36303.0
62	34430.5
63	32558.0
64	30078.5
65	27599.0
66	26236.5
67	24874.0
68	24874.0
69	19707.0
70	14540.0
71	12747.5
72	10955.0
73	7942.0
74	4929.0
75	4929.0
76	3826.5
77	2724.0
78	2055.0
79	1386.0
80	1129.0
81	872.0
82	872.0
83	767.0
84	662.0
85	586.5
86	511.0
87	418.5
88	326.0
89	326.0
90	244.5
91	163.0
92	100.5
93	38.0
94	19.5
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1123305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.458088307980596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73283338051125	15.084328164961185
2	10.657117321941037	4.360484945615588
3	4.0069186913131025	2.4592168928934446
4	2.000116976410317	1.636742789187736
5	1.324722378765176	1.3550643704168044
6	0.970698437788315	1.1915180616415293
7	0.7584340264068534	1.0861277201606088
8	0.5724353962371638	0.9368747109467008
9	0.478605204888453	0.8812212791640404
>10	4.102160980780386	17.790267389150884
>50	0.7095569643286216	10.195559909437028
>100	0.6003271359800886	24.058779041177257
>500	0.05942102655989232	8.433417337608516
>1k	0.02490440083760193	7.299474928628012
>5k	8.738386258807696E-4	1.2395958795318966
>10k+	8.738386258807696E-4	1.9913265794787707
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11227	0.9994614107477487	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11051	0.9837933597731694	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8736	0.7777050756473086	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5132	0.45686612273603333	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	2856	0.2542497362693124	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2335	0.20786874446388115	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2257	0.2009249491456016	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	2246	0.1999456959596904	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2192	0.1951384530470353	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1914	0.1703900543485518	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1860	0.16558281143589673	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1819	0.16193286774295496	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1720	0.15311958906975398	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1706	0.1518732668331397	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	1632	0.14528556358246425	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	1628	0.14492947151486016	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1619	0.144128264362751	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1603	0.1427038960923347	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1594	0.14190268894022548	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	1573	0.14003320558530408	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1572	0.13994418256840307	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	1554	0.1383417682641847	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1543	0.1373625150782735	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	1510	0.13442475552053984	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	1509	0.1343357325036388	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1437	0.1279260752867654	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1402	0.1248102696952297	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1391	0.12383101650931848	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	1384	0.12320785539101135	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	1369	0.12187251013749605	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1321	0.11759940532624709	No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG	1318	0.11733233627554404	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1314	0.11697624420793995	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1300	0.11572992197132567	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	1296	0.11537382990372161	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1280	0.1139494616333053	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1274	0.11341532353189916	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1260	0.1121690012952849	No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA	1240	0.11038854095726451	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	1223	0.10887514966994717	No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT	1217	0.10834101156854103	No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA	1211	0.10780687346713493	No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC	1185	0.10549227502770842	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	1172	0.10433497580799515	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1172	0.10433497580799515	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	1161	0.10335572262208394	No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG	1159	0.1031776765882819	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1151	0.10246549245307374	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	1150	0.10237646943617273	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1149	0.1022874464192717	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.10202037736856864	No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT	1135	0.10104112418265743	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	1124	0.1000618709967462	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.792531859112174E-4	0.0
3	0.0	0.0	0.0	0.001780460338020395	0.0
4	0.0	0.0	0.0	0.003026782574634672	0.0
5	0.0	0.0	0.0	0.0031158055915356917	0.0
6	0.0	0.0	0.0	0.0031158055915356917	0.0
7	8.902301690101976E-5	0.0	0.0	0.0034718976591397704	8.902301690101976E-5
8	8.902301690101976E-5	0.0	0.0	0.004006035760545889	8.902301690101976E-5
9	8.902301690101976E-5	0.0	0.0	0.006943795318279541	8.902301690101976E-5
10	2.6706905070305925E-4	0.0	0.0	0.010415692977419311	8.902301690101976E-5
11	2.6706905070305925E-4	0.0	0.0	0.012196153315439707	8.902301690101976E-5
12	2.6706905070305925E-4	0.0	0.0	0.013620521585856023	1.7804603380203952E-4
13	2.6706905070305925E-4	0.0	0.0	0.014421728737965201	1.7804603380203952E-4
14	2.6706905070305925E-4	0.0	0.0	0.015935120025282537	1.7804603380203952E-4
15	2.6706905070305925E-4	0.0	0.0	0.01887287958301619	1.7804603380203952E-4
16	2.6706905070305925E-4	0.0	0.0	0.02563862886749369	1.7804603380203952E-4
17	2.6706905070305925E-4	0.0	0.0	0.03454093055759567	1.7804603380203952E-4
18	2.6706905070305925E-4	0.0	0.0	0.03819087425053748	1.7804603380203952E-4
19	2.6706905070305925E-4	0.0	0.0	0.045045646551916	1.7804603380203952E-4
20	2.6706905070305925E-4	0.0	0.0	0.050030935498373105	1.7804603380203952E-4
21	2.6706905070305925E-4	0.0	0.0	0.06311731898282301	1.7804603380203952E-4
22	2.6706905070305925E-4	0.0	0.0	0.08661939544469223	1.7804603380203952E-4
23	2.6706905070305925E-4	0.0	0.0	0.12650170701634908	1.7804603380203952E-4
24	2.6706905070305925E-4	0.0	0.0	0.1775118957006334	1.7804603380203952E-4
25	2.6706905070305925E-4	0.0	0.0	0.19113241728648941	1.7804603380203952E-4
26	2.6706905070305925E-4	0.0	0.0	0.21338817151174436	1.7804603380203952E-4
27	2.6706905070305925E-4	0.0	0.0	0.2311927748919483	1.7804603380203952E-4
28	3.5609206760407904E-4	0.0	0.0	0.2563862886749369	1.7804603380203952E-4
29	3.5609206760407904E-4	0.0	0.0	0.29306377163815706	1.7804603380203952E-4
30	3.5609206760407904E-4	0.0	0.0	0.36081028749983307	1.7804603380203952E-4
31	3.5609206760407904E-4	0.0	0.0	0.5880860496481365	1.7804603380203952E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGGT	20	0.0018419183	37.0	11
CTGTGCG	25	1.233608E-4	36.999996	9
GTCGTCA	30	3.5994273E-4	30.833332	24
ACTGTGC	30	3.5994273E-4	30.833332	8
TCGTTCG	25	0.0054962914	29.599998	23
CGTCTCT	25	0.0054962914	29.599998	20
GCTCTAG	25	0.0054962914	29.599998	1
CGTATAA	40	5.9400125E-5	27.75	30
TCCCAAC	65	9.391442E-8	25.615383	33
TCGTATA	45	1.3231859E-4	24.666668	29
CGTCCCA	70	1.9228355E-7	23.785715	31
TACCCTT	80	2.7257556E-8	23.125	5
TCGTCCC	75	3.7406062E-7	22.2	30
AATCGTC	75	3.7406062E-7	22.2	28
CTTCTGT	60	3.7261896E-5	21.583332	37
AATTAGT	70	5.1017505E-6	21.142859	6
CGTTTGG	105	9.822543E-10	21.142857	28
GTATGTA	160	0.0	20.8125	1
GTATTGA	45	0.0038257542	20.555557	6
AACCACG	45	0.0038257542	20.555557	7
>>END_MODULE