FastQCFastQC Report
Fri 10 Feb 2017
ERR1630679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721161
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58730.3412231627372454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.3111852987605459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44100.2562223987180746No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG40680.23635209024606066No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT38390.22304711761421508No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG33600.1952170656899616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30590.17772887022190254No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA29470.1712216346989038No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC27410.15925296936195973No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC25320.14711000307350677No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT23740.13793015296070502No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23080.13409553202750935No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC21910.12729779491866247No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC21900.12723969460149284No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC21270.12357937461980606No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA20920.12154586351886894No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT20870.12125536193302079No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC19590.11381852133530797No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC19230.11172690991720123No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC19020.11050680325663897No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG18820.10934479691324635No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG18710.1087056934243804No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA18430.10707888454363071No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA18420.10702078422646111No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT18180.10562637661438994No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC17590.10219845790138168No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA17450.10138505346100685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA1600.024.2812515
AACACCG609.2393596E-418.55
GTAGTAC752.0680198E-417.2666663
CCGAATT2450.016.61224614
GGTATCA27200.016.2555141
TATAGGT803.3837886E-416.18754
CGAAATT700.00259319615.85714325
CCCGAAT2700.015.75925913
GTCGGTT957.0629365E-515.57894812
TAGTACT957.0629365E-515.5789484
ACTTTTG8150.015.43558230
CCGCTAC1802.0190782E-1015.4166661
TCGCCAT7750.015.27741813
GATGTGC7750.015.27741836
GGATCGA855.366075E-415.23529419
GTGCGTA1101.452624E-515.13636415
CTATTAG1353.975183E-715.0740751
CTAGACC1001.09386536E-414.83
TCGATGC1752.240995E-914.829
TCTAGCG1508.1077815E-814.79999928
Produced by FastQC (version 0.11.5)