##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630679.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1721161
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.674834021918926	31.0	31.0	34.0	30.0	34.0
2	31.870958614563076	33.0	31.0	34.0	30.0	34.0
3	31.95147635810944	33.0	31.0	34.0	30.0	34.0
4	35.640941782901194	37.0	35.0	37.0	33.0	37.0
5	35.52166066974559	37.0	35.0	37.0	33.0	37.0
6	35.5977784762727	37.0	35.0	37.0	33.0	37.0
7	35.55851195791678	37.0	35.0	37.0	33.0	37.0
8	35.549825379496745	37.0	35.0	37.0	33.0	37.0
9	37.192406753348465	39.0	37.0	39.0	34.0	39.0
10	37.047267513033354	39.0	37.0	39.0	33.0	39.0
11	37.15737168109201	39.0	37.0	39.0	33.0	39.0
12	37.100478688513164	39.0	37.0	39.0	33.0	39.0
13	37.146632999469546	39.0	37.0	39.0	33.0	39.0
14	38.35277001977154	40.0	38.0	41.0	34.0	41.0
15	38.35923193704714	40.0	38.0	41.0	34.0	41.0
16	38.31237228824032	40.0	38.0	41.0	34.0	41.0
17	38.28251744026271	40.0	38.0	41.0	33.0	41.0
18	38.247155844223755	40.0	38.0	41.0	33.0	41.0
19	38.3248801245206	40.0	38.0	41.0	34.0	41.0
20	38.28603890048636	40.0	38.0	41.0	34.0	41.0
21	38.234200054498096	40.0	38.0	41.0	34.0	41.0
22	38.182614525892696	40.0	38.0	41.0	34.0	41.0
23	38.124080199353806	40.0	38.0	41.0	33.0	41.0
24	38.06005248782653	40.0	37.0	41.0	33.0	41.0
25	38.00785341987182	40.0	37.0	41.0	33.0	41.0
26	37.85650383665445	40.0	37.0	41.0	33.0	41.0
27	37.71422371294725	40.0	37.0	41.0	33.0	41.0
28	37.62653116123361	40.0	37.0	41.0	32.0	41.0
29	37.55081192288229	39.0	37.0	41.0	32.0	41.0
30	37.46227401155383	39.0	37.0	41.0	32.0	41.0
31	37.41016267507804	39.0	36.0	41.0	32.0	41.0
32	37.31010521386436	39.0	36.0	41.0	32.0	41.0
33	37.24656496399814	39.0	36.0	40.0	31.0	41.0
34	37.18103710228154	39.0	36.0	40.0	31.0	41.0
35	37.14633784985832	39.0	36.0	40.0	31.0	41.0
36	37.11237182343778	39.0	36.0	40.0	31.0	41.0
37	36.97658266716478	39.0	36.0	40.0	31.0	41.0
38	36.817922902041126	39.0	35.0	40.0	30.0	41.0
39	36.757961050709376	39.0	35.0	40.0	30.0	41.0
40	36.58644659041194	39.0	35.0	40.0	30.0	41.0
41	36.536938729148524	39.0	35.0	40.0	30.0	41.0
42	36.40811173388195	39.0	35.0	40.0	30.0	41.0
43	35.580138057973656	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	7.0
11	2.0
12	0.0
13	5.0
14	7.0
15	13.0
16	15.0
17	41.0
18	84.0
19	165.0
20	327.0
21	600.0
22	1077.0
23	1983.0
24	3213.0
25	5118.0
26	7868.0
27	11436.0
28	16296.0
29	22569.0
30	29607.0
31	38875.0
32	49388.0
33	63546.0
34	83493.0
35	113841.0
36	163314.0
37	260521.0
38	476237.0
39	371509.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.77699761963	20.100327627688518	12.759701155208605	24.362973597472866
2	18.176393724933344	22.098688036737993	35.85422862823408	23.87068961009458
3	20.344929962972667	24.406781236618773	30.717695787901306	24.530593012507254
4	14.990869535156792	16.064563396451582	34.65033195616215	34.29423511222948
5	13.614589222042564	38.300426282027075	34.56934011402768	13.51564438190268
6	33.92489139598213	35.24969482808407	16.145613338903217	14.679800437030584
7	27.498822016069386	31.703193367732595	22.16021627262063	18.637768343577388
8	25.914484467170706	35.004801991214066	20.65791637156547	18.42279717004975
9	25.962242927884144	14.61466998148343	20.33999143601325	39.08309565461918
10	16.205049963367752	28.214966525502263	33.7387960800878	21.841187431042187
11	34.230324763342885	22.4933634912713	22.67765769733337	20.59865404805245
12	20.499709207912566	26.751477636316416	29.526929787509708	23.221883368261306
13	28.757216785646435	20.68946484378858	26.786570227886873	23.76674814267811
14	22.045584346844947	22.014500677159194	25.929067646780286	30.01084732921557
15	24.876173699032222	28.77371727572261	23.759950405569263	22.590158619675904
16	24.382611504676206	27.776541532140225	24.671079579423424	23.16976738376015
17	22.973272111092456	27.764572866803277	26.130036643870042	23.132118378234225
18	21.832356182832402	26.204521250481505	28.284686906105822	23.67843566058027
19	23.504309010022887	26.85460569929251	28.03747005654904	21.60361523413556
20	23.69888697222398	25.63171022350611	28.391591489697944	22.277811314571967
21	23.137521707731	27.159922866018928	26.831888475279186	22.870666950970886
22	23.37875422461931	26.306487307114207	27.074689700731074	23.2400687675354
23	22.5645363798041	26.98143869167382	27.40824362160193	23.045781306920155
24	24.20046701034941	26.745900005868133	26.99212915003303	22.06150383374943
25	23.629108491303256	25.90925543862544	27.569530102064828	22.89210596800648
26	23.515638571870966	26.591295061879745	27.060919925561873	22.83214644068742
27	23.542597119037673	26.139448895251522	26.856987812296467	23.46096617341434
28	22.58492959113064	26.604716235145926	27.678468196757887	23.13188597696555
29	22.67771579765054	27.33137690198651	27.397727464194226	22.593179836168726
30	22.17334694430097	27.991745106936538	27.25381297856505	22.581094970197444
31	23.06565161539217	26.906663583476504	27.451295956624627	22.576388844506702
32	22.246669544569045	26.95116842642844	27.49045557039696	23.311706458605556
33	22.45513348257368	26.554982363648723	27.714722794671736	23.27516135910586
34	22.72181393838229	26.83624599906691	27.304650756088478	23.137289306462325
35	23.12026591353162	26.73050342181818	27.281294428586282	22.867936236063912
36	22.655637677126077	27.136624638833908	27.28042292382874	22.927314760211274
37	23.66611839334031	25.431612731173896	27.372744327811287	23.529524547674505
38	22.727275368196235	26.19028667277495	28.1928884049778	22.889549554051015
39	22.705313448306114	26.050613510299154	28.225366482275625	23.018706559119106
40	22.50614556104862	25.323952843458574	28.94674002025377	23.22316157523904
41	21.75252634704133	26.403805338373342	28.944590308518492	22.899078006066837
42	22.791941021206036	25.289208853791134	28.549217650179155	23.36963247482368
43	21.650850791994475	25.701430604109664	28.618008425708	24.029710178187862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	219.0
2	228.0
3	566.0
4	904.0
5	904.0
6	1375.5
7	1847.0
8	1931.5
9	2016.0
10	3049.0
11	4082.0
12	4082.0
13	6479.0
14	8876.0
15	12987.0
16	17098.0
17	17057.5
18	17017.0
19	17017.0
20	19996.0
21	22975.0
22	24401.0
23	25827.0
24	27844.5
25	29862.0
26	29862.0
27	32121.0
28	34380.0
29	45076.0
30	55772.0
31	57326.5
32	58881.0
33	58881.0
34	64109.5
35	69338.0
36	72836.0
37	76334.0
38	90411.0
39	104488.0
40	104488.0
41	111084.0
42	117680.0
43	109057.0
44	100434.0
45	106742.5
46	113051.0
47	113051.0
48	119238.0
49	125425.0
50	128507.0
51	131589.0
52	136241.5
53	140894.0
54	140894.0
55	119649.5
56	98405.0
57	91309.5
58	84214.0
59	76093.0
60	67972.0
61	67972.0
62	63390.0
63	58808.0
64	52762.5
65	46717.0
66	40806.5
67	34896.0
68	34896.0
69	30390.5
70	25885.0
71	22030.0
72	18175.0
73	14767.0
74	11359.0
75	11359.0
76	8835.0
77	6311.0
78	5165.0
79	4019.0
80	3146.0
81	2273.0
82	2273.0
83	1742.0
84	1211.0
85	1010.0
86	809.0
87	680.0
88	551.0
89	551.0
90	415.0
91	279.0
92	163.5
93	48.0
94	31.0
95	14.0
96	14.0
97	9.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1721161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.62671666587537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.28957392227917	41.959786623377774
2	7.990480717985907	7.771016837952822
3	2.183866875667471	3.1858282739721777
4	0.9550202392974185	1.8575799434596827
5	0.5351899933535039	1.3012266084606279
6	0.34916246915888743	1.0187174674888002
7	0.23645719417597857	0.804869588936223
8	0.17434985816890036	0.678244892313174
9	0.12529276469226994	0.5483318192077684
>10	0.8656429398104895	8.270452637655229
>50	0.12513847045062065	4.282483910384979
>100	0.1422596340104137	14.88263692821285
>500	0.019894682076258562	6.512380009844744
>1k	0.007430543908000187	6.272041968012751
>5k	2.3969496477419953E-4	0.654402490720526
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5873	0.3412231627372454	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5356	0.3111852987605459	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4410	0.2562223987180746	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4068	0.23635209024606066	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3839	0.22304711761421508	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3360	0.1952170656899616	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3059	0.17772887022190254	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2947	0.1712216346989038	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2741	0.15925296936195973	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2532	0.14711000307350677	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2374	0.13793015296070502	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2308	0.13409553202750935	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2191	0.12729779491866247	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2190	0.12723969460149284	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2127	0.12357937461980606	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	2092	0.12154586351886894	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2087	0.12125536193302079	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1959	0.11381852133530797	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1923	0.11172690991720123	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1902	0.11050680325663897	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1882	0.10934479691324635	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1871	0.1087056934243804	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1843	0.10707888454363071	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	1842	0.10702078422646111	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1818	0.10562637661438994	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	1759	0.10219845790138168	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1745	0.10138505346100685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.810031716963143E-5	0.0
2	0.0	0.0	0.0	6.391034888659457E-4	0.0
3	0.0	0.0	0.0	9.877053918837342E-4	0.0
4	0.0	0.0	0.0	0.0019173104665978372	0.0
5	0.0	5.810031716963143E-5	0.0	0.0022659123696156256	0.0
6	0.0	5.810031716963143E-5	0.0	0.002324012686785257	0.0
7	5.810031716963143E-5	1.1620063433926286E-4	0.0	0.002614514272633414	0.0
8	5.810031716963143E-5	1.1620063433926286E-4	0.0	0.00284691554131194	0.0
9	5.810031716963143E-5	1.1620063433926286E-4	0.0	0.00447372442206162	0.0
10	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.0069720380603557714	0.0
11	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.00772734218356098	0.0
12	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.008308345355257294	0.0
13	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.00935415106431066	0.0
14	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.009993254553176605	0.0
15	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.011155260896569234	0.0
16	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.01342117326618486	0.0
17	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.016093787855987907	0.0
18	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.0171976938822109	0.0
19	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.019289305300317635	0.0
20	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.02074181322955842	0.0
21	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.024111631625397045	0.0
22	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.030560766831226133	0.0
23	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.039101513455161954	0.0
24	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.05275508799002534	0.0
25	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.056415407971712114	0.0
26	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.06205113873716637	0.0
27	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.07297399836505708	0.0
28	5.810031716963143E-5	1.7430095150889428E-4	0.0	0.09528452015819554	0.0
29	5.810031716963143E-5	2.3240126867852572E-4	0.0	0.13066761331450108	0.0
30	5.810031716963143E-5	2.3240126867852572E-4	0.0	0.18388750384188346	0.0
31	5.810031716963143E-5	2.3240126867852572E-4	0.0	0.37503754732997086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	160	0.0	24.28125	15
AACACCG	60	9.2393596E-4	18.5	5
GTAGTAC	75	2.0680198E-4	17.266666	3
CCGAATT	245	0.0	16.612246	14
GGTATCA	2720	0.0	16.255514	1
TATAGGT	80	3.3837886E-4	16.1875	4
CGAAATT	70	0.002593196	15.857143	25
CCCGAAT	270	0.0	15.759259	13
GTCGGTT	95	7.0629365E-5	15.578948	12
TAGTACT	95	7.0629365E-5	15.578948	4
ACTTTTG	815	0.0	15.435582	30
CCGCTAC	180	2.0190782E-10	15.416666	1
TCGCCAT	775	0.0	15.277418	13
GATGTGC	775	0.0	15.277418	36
GGATCGA	85	5.366075E-4	15.235294	19
GTGCGTA	110	1.452624E-5	15.136364	15
CTATTAG	135	3.975183E-7	15.074075	1
CTAGACC	100	1.09386536E-4	14.8	3
TCGATGC	175	2.240995E-9	14.8	29
TCTAGCG	150	8.1077815E-8	14.799999	28
>>END_MODULE