FastQCFastQC Report
Fri 10 Feb 2017
ERR1630678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134823
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30262.2444241709500603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28992.1502265933854012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21971.6295439205476812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.9085986812339141No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT11460.8500033377094411No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT7970.5911454277089221Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC5430.4027502725796044No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG5060.3753068838402943No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4840.3589891932385424No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC4190.3107778346424572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.26924189492890677No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.23808994014374402No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2940.2180636834961394No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA2550.18913686833848822No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2500.18542830229263552No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.18542830229263552No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2480.18394487587429445No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2470.1832031626651239No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2400.17801117020093013No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.1757860305734185No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2060.15279292108913167No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2040.15130949467079058No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA1920.1424089361607441No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1860.13795865690572084No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1710.1268329587681627No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC1700.12609124555899215Illumina PCR Primer Index 11 (95% over 23bp)
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1660.12312439272230999No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC1650.12238267951313944No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1630.12089925309479836No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1560.11570726063060456No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1530.11348212100309296No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.10977355495724024No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.10977355495724024No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1470.1090318417480697No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG1450.10754841532972861No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1430.10606498891138753No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1420.10532327570221699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC358.828671E-426.42857230
ATGCCCT451.314766E-424.66666837
CCGTCTT1002.0008883E-1124.05000137
CGTCTTC400.001922404923.12537
ATACCAG502.684995E-422.25
GCCGTCT1107.2759576E-1121.86363636
AAAAAGT450.00380857520.5555571
GAGCTGT1202.3283064E-1020.04166635
AGTCGGT4000.018.96250211
CAGTCGG4050.018.72839510
TGGGCAG4150.018.722896
TAGAGCT1205.0549716E-918.533
GCGCGAT500.007003499718.513
CGAGCTC500.007003499718.528
GGGCAGT4200.018.57
ATACACT500.007003499718.54
GGCAGTC4150.018.277118
GCAGTCG4200.018.0595239
AGAGCTG1258.383722E-917.7634
CTCGCCT3200.017.3437523
Produced by FastQC (version 0.11.5)