FastQCFastQC Report
Fri 10 Feb 2017
ERR1630677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337884
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88460.661193347106326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77160.5767316149980117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61970.4631941184736494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.3428548364432193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG358.8701595E-426.4285731
TCGCCTA500.0070354718.535
CGAATTA1306.9667294E-1018.515
CTAGCGG1205.175025E-918.529
GTATTAG2200.017.659091
TAGGACA2850.017.5263164
CGCAATA1301.3935278E-817.07692336
GGTATCA42200.017.0533181
CGTTTAG1005.8792393E-616.65000226
TCTAGCG1452.9795046E-916.58620628
CTCGATT1708.54925E-1116.32352825
TATTGTC3100.016.1129025
ATTGCCG2900.015.94827611
TACATCG700.002592791615.8571425
GTTAGTC957.061057E-515.5789483
TGTACTA957.061057E-515.5789485
CCGCTAC3150.015.2698411
CTTATAC13500.014.937036537
CTTAGGA3100.014.9193542
CTAGGAC1405.996444E-714.5357143
Produced by FastQC (version 0.11.5)