##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630677.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337884
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06982817643383	33.0	31.0	34.0	30.0	34.0
2	32.23915825288291	33.0	31.0	34.0	30.0	34.0
3	32.335970084102954	34.0	31.0	34.0	30.0	34.0
4	35.91967614531604	37.0	35.0	37.0	35.0	37.0
5	35.88052775875936	37.0	35.0	37.0	35.0	37.0
6	35.958977011459886	37.0	35.0	37.0	35.0	37.0
7	35.90387058967743	37.0	35.0	37.0	35.0	37.0
8	35.914180153137345	37.0	35.0	37.0	35.0	37.0
9	37.617762825476646	39.0	37.0	39.0	35.0	39.0
10	37.5278731190447	39.0	37.0	39.0	35.0	39.0
11	37.628802646567266	39.0	37.0	39.0	35.0	39.0
12	37.58631241572513	39.0	37.0	39.0	35.0	39.0
13	37.619067123906106	39.0	37.0	39.0	35.0	39.0
14	38.90917149767842	40.0	38.0	41.0	36.0	41.0
15	38.91764308415378	40.0	38.0	41.0	36.0	41.0
16	38.87661486347098	40.0	38.0	41.0	35.0	41.0
17	38.88158614648206	40.0	38.0	41.0	35.0	41.0
18	38.880725832732885	40.0	38.0	41.0	35.0	41.0
19	38.95086420048375	40.0	38.0	41.0	35.0	41.0
20	38.923802811006034	40.0	38.0	41.0	35.0	41.0
21	38.88426051884917	40.0	38.0	41.0	35.0	41.0
22	38.806815090097494	40.0	38.0	41.0	35.0	41.0
23	38.750832658137774	40.0	38.0	41.0	35.0	41.0
24	38.69265347369428	40.0	38.0	41.0	34.0	41.0
25	38.645819069515746	40.0	38.0	41.0	34.0	41.0
26	38.49825321178817	40.0	38.0	41.0	34.0	41.0
27	38.355019568213685	40.0	38.0	41.0	34.0	41.0
28	38.264591698532904	40.0	38.0	41.0	34.0	41.0
29	38.17869635932562	40.0	38.0	41.0	34.0	41.0
30	38.074660433938966	40.0	37.0	41.0	33.0	41.0
31	38.00059795916537	40.0	37.0	41.0	33.0	41.0
32	37.885499789219395	40.0	37.0	41.0	33.0	41.0
33	37.788252195257584	40.0	37.0	41.0	33.0	41.0
34	37.70313943510798	40.0	37.0	41.0	33.0	41.0
35	37.62448014925061	40.0	37.0	41.0	33.0	41.0
36	37.5626960184889	40.0	37.0	41.0	33.0	41.0
37	37.39808010260979	40.0	36.0	41.0	32.0	41.0
38	37.228238771074324	40.0	36.0	41.0	32.0	41.0
39	37.13497209025596	40.0	36.0	41.0	31.0	41.0
40	36.93622765501344	40.0	36.0	41.0	31.0	41.0
41	36.83997192581719	40.0	35.0	41.0	31.0	41.0
42	36.67541206860984	40.0	35.0	41.0	30.0	41.0
43	35.893729949681735	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	9.0
11	6.0
12	7.0
13	2.0
14	1.0
15	2.0
16	5.0
17	20.0
18	49.0
19	76.0
20	157.0
21	361.0
22	654.0
23	1120.0
24	1845.0
25	2993.0
26	4695.0
27	7014.0
28	10163.0
29	14372.0
30	19239.0
31	24522.0
32	32032.0
33	41055.0
34	54229.0
35	72559.0
36	102252.0
37	162783.0
38	290648.0
39	495003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1181746698518	19.44137159873352	12.434187119361619	24.00626661205306
2	18.075333885448963	21.53191158575781	35.57490783954364	24.817846689249592
3	20.243533819075495	24.07420972221807	29.942057756875784	25.740198701830654
4	13.940521001820786	15.777302068041774	34.47840022004897	35.80377671008847
5	13.6360850417525	38.36109857057862	34.12440839415076	13.878407993518122
6	33.906003808999884	34.787395618753195	16.324285214562696	14.982315357684223
7	28.210517503759668	31.53031204499045	21.763321782755455	18.49584866849443
8	27.57555961503389	33.48152754648385	20.356772336017173	18.58614050246509
9	26.72548591656676	13.450194486218535	20.729151406250466	39.095168190964245
10	16.589928573777694	27.95601113399966	33.3229936227655	22.13106666945714
11	33.931940287797744	22.417788089251385	22.113202639391755	21.537068983559113
12	20.924609308430327	26.776013466040403	29.981074592416086	22.318302633113184
13	29.632838123484547	20.29099682782663	26.611724185355385	23.464440863333444
14	22.485880689207736	21.896068717467283	27.045169835351945	28.57288075797304
15	25.384263508644995	27.986432306537782	23.877705391498814	22.751598793318404
16	23.979956408776847	27.16790095404385	25.723605334991674	23.12853730218763
17	22.130468710291773	27.720340477948763	27.247055798559515	22.90213501319995
18	21.982997031132744	25.723605334991674	29.06380523274066	23.229592401134926
19	22.652935531032586	26.506931841624535	29.02927309094062	21.81085953640226
20	23.42034137488751	25.473060444702234	28.964842990872153	22.141755189538106
21	23.79249621043379	25.931396144957258	27.867214197942424	22.40889344666653
22	23.51511790259843	26.000310938765992	27.918713431059793	22.565857727575782
23	22.90153705403458	26.145091801680863	28.128372863417155	22.8249982808674
24	22.795474047077324	26.551479799444493	28.225167503311198	22.42787865016698
25	22.941151848740247	26.15749945436226	28.187271841205963	22.714076855691527
26	22.6412753273079	26.816599944389797	28.178078219038422	22.364046509263883
27	22.900789605077872	26.599391277569655	27.621154001393244	22.878665115959233
28	22.268373042804907	26.67764918333727	28.504638668225347	22.549339105632477
29	21.898909023502785	27.088372385049826	28.739487130423864	22.273231461023528
30	22.013717183253554	27.599477981648633	28.549261370940975	21.837543464156834
31	22.298121511281995	27.327331816510252	27.929102971558073	22.445443700649683
32	21.640366429376538	27.109375700733395	28.430790711302322	22.819467158587738
33	21.80839295484511	26.8352114234119	28.66690983672725	22.68948578501574
34	22.453889873860515	26.569343829509883	28.225092758415528	22.751673538214074
35	22.19205850432474	26.718459896373677	28.757351160489254	22.332130438812335
36	21.708234794645872	26.918103512711117	28.313964439368434	23.059697253274575
37	22.222255442175854	25.9868568575452	28.581700655662225	23.209187044616723
38	21.715260814838956	25.88894104421609	29.32892537768596	23.066872763258996
39	21.76302280317277	25.971982623306655	29.2890116033976	22.97598297012297
40	21.10280113971017	25.79573415931426	30.02539831554903	23.07606638542654
41	20.63123559292136	25.93057395110488	30.263984022531105	23.17420643344266
42	21.045397059834784	25.770395639681766	30.02965877460228	23.154548525881165
43	20.403562640707264	25.54197523851096	30.11389627202358	23.94056584875819
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	308.0
2	286.0
3	885.0
4	1484.0
5	1484.0
6	2079.0
7	2674.0
8	2705.5
9	2737.0
10	4038.5
11	5340.0
12	5340.0
13	8851.5
14	12363.0
15	17116.5
16	21870.0
17	20641.0
18	19412.0
19	19412.0
20	21900.0
21	24388.0
22	22647.5
23	20907.0
24	22740.5
25	24574.0
26	24574.0
27	27238.5
28	29903.0
29	33939.5
30	37976.0
31	42061.0
32	46146.0
33	46146.0
34	49740.5
35	53335.0
36	57142.5
37	60950.0
38	65967.0
39	70984.0
40	70984.0
41	73916.5
42	76849.0
43	77176.0
44	77503.0
45	80355.0
46	83207.0
47	83207.0
48	84786.5
49	86366.0
50	87985.0
51	89604.0
52	90437.0
53	91270.0
54	91270.0
55	86948.5
56	82627.0
57	78705.0
58	74783.0
59	68407.0
60	62031.0
61	62031.0
62	57189.5
63	52348.0
64	46305.5
65	40263.0
66	34470.5
67	28678.0
68	28678.0
69	24672.0
70	20666.0
71	17735.5
72	14805.0
73	11869.0
74	8933.0
75	8933.0
76	6988.5
77	5044.0
78	4128.0
79	3212.0
80	2510.0
81	1808.0
82	1808.0
83	1381.5
84	955.0
85	776.0
86	597.0
87	508.0
88	419.0
89	419.0
90	324.5
91	230.0
92	127.0
93	24.0
94	13.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1337884.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.12088785881931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.44672974169764	55.19715221646175
2	7.822028065450287	9.874667126956497
3	1.9393085172868234	3.6723262632994413
4	0.7847331900486816	1.9813222275262548
5	0.422430859631815	1.3332105459462216
6	0.26117445947008594	0.9891338260679425
7	0.20160860106167947	0.8907999729291395
8	0.13190391051728606	0.6660713555121079
9	0.10037429505751728	0.570214316001023
>10	0.7125245498446677	8.892993429941232
>50	0.0998824630952359	4.4327163338570275
>100	0.07433735485954798	8.387116056278336
>500	0.0022526339038582708	0.8995277839103134
>1k	3.556790374513059E-4	0.509557793730892
>5k	3.556790374513059E-4	1.7031907515819316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8846	0.661193347106326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7716	0.5767316149980117	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6197	0.4631941184736494	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4587	0.3428548364432193	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.4948979134214924E-4	0.0
3	0.0	0.0	0.0	4.4846937402644773E-4	0.0
4	0.0	0.0	0.0	8.969387480528955E-4	0.0
5	0.0	7.474489567107462E-5	0.0	9.716836437239701E-4	0.0
6	0.0	7.474489567107462E-5	0.0	9.716836437239701E-4	0.0
7	0.0	7.474489567107462E-5	0.0	0.0010464285393950448	0.0
8	0.0	7.474489567107462E-5	0.0	0.0011211734350661192	0.0
9	0.0	7.474489567107462E-5	0.0	0.0017191326004347162	0.0
10	0.0	7.474489567107462E-5	0.0	0.003139285618185134	0.0
11	7.474489567107462E-5	7.474489567107462E-5	0.0	0.003961479470566955	0.0
12	7.474489567107462E-5	7.474489567107462E-5	0.0	0.005531122279659522	0.0
13	7.474489567107462E-5	7.474489567107462E-5	0.0	0.00583010186234382	0.0
14	7.474489567107462E-5	7.474489567107462E-5	0.0	0.007250254880094238	0.0
15	7.474489567107462E-5	7.474489567107462E-5	0.0	0.01016530581126615	0.0
16	7.474489567107462E-5	7.474489567107462E-5	0.0	0.016219642360623194	0.0
17	7.474489567107462E-5	7.474489567107462E-5	0.0	0.024665815571454624	0.0
18	7.474489567107462E-5	7.474489567107462E-5	0.0	0.02810408077232406	0.0
19	7.474489567107462E-5	7.474489567107462E-5	0.0	0.03348571326064143	0.0
20	7.474489567107462E-5	7.474489567107462E-5	0.0	0.037297702939866235	0.0
21	7.474489567107462E-5	7.474489567107462E-5	0.0	0.045295406776671224	0.0
22	7.474489567107462E-5	7.474489567107462E-5	0.0	0.06502805923383492	0.0
23	7.474489567107462E-5	7.474489567107462E-5	0.0	0.0942533134412251	0.0
24	7.474489567107462E-5	1.4948979134214924E-4	0.0	0.1481443832200699	0.0
25	7.474489567107462E-5	1.4948979134214924E-4	0.0	0.15913188288371788	0.0
26	7.474489567107462E-5	1.4948979134214924E-4	0.0	0.17624846399239397	0.0
27	7.474489567107462E-5	1.4948979134214924E-4	7.474489567107462E-5	0.2002415755028089	0.0
28	7.474489567107462E-5	1.4948979134214924E-4	7.474489567107462E-5	0.24957320664571817	0.0
29	7.474489567107462E-5	1.4948979134214924E-4	7.474489567107462E-5	0.32476657169081924	0.0
30	7.474489567107462E-5	1.4948979134214924E-4	7.474489567107462E-5	0.44047167018964273	0.0
31	7.474489567107462E-5	1.4948979134214924E-4	7.474489567107462E-5	0.8242867094606109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	35	8.8701595E-4	26.42857	31
TCGCCTA	50	0.00703547	18.5	35
CGAATTA	130	6.9667294E-10	18.5	15
CTAGCGG	120	5.175025E-9	18.5	29
GTATTAG	220	0.0	17.65909	1
TAGGACA	285	0.0	17.526316	4
CGCAATA	130	1.3935278E-8	17.076923	36
GGTATCA	4220	0.0	17.053318	1
CGTTTAG	100	5.8792393E-6	16.650002	26
TCTAGCG	145	2.9795046E-9	16.586206	28
CTCGATT	170	8.54925E-11	16.323528	25
TATTGTC	310	0.0	16.112902	5
ATTGCCG	290	0.0	15.948276	11
TACATCG	70	0.0025927916	15.857142	5
GTTAGTC	95	7.061057E-5	15.578948	3
TGTACTA	95	7.061057E-5	15.578948	5
CCGCTAC	315	0.0	15.269841	1
CTTATAC	1350	0.0	14.9370365	37
CTTAGGA	310	0.0	14.919354	2
CTAGGAC	140	5.996444E-7	14.535714	3
>>END_MODULE