FastQCFastQC Report
Fri 10 Feb 2017
ERR1630676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125418
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35492.829737358273932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26072.078648997751519No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22111.7629048461943262No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT15801.2597872713645568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14621.1657018928702418No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT13561.0811845189685692RNA PCR Primer, Index 27 (95% over 23bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC7850.6259069671020109RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC6190.49354957023712703No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG5930.4728188936197356No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.3715575116809389No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.3372721618906377No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3950.3149468178411392No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3570.28464813663110555No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.28145880176689153No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC3340.2663094611618747RNA PCR Primer, Index 27 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2570.20491476502575387No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.19534676043311167No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.16903474780334563No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2090.16664274665518505No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1910.15229073976622176No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG1900.15149340605016823No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.13076272943277678No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.12916806200066977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1570.12518139342040216No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1490.11880272369197405No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1460.11641072254381349No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1390.11082938653143887No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1290.1028560493709037No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1270.10126138193879666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCA200.001835690237.07
GCACAGG200.001835690237.01
ATGCCCC200.001835690237.037
AGTGGCT556.1872925E-726.9090927
AGGACCG358.8252197E-426.4285725
CCGTCTT2000.025.90000237
CAGTGGC551.8851952E-523.54545426
TGCGTCT400.001921659923.12500235
GCCGTCT2250.023.02222336
TGGCTGT705.042377E-621.14285929
TCCGCTT450.003807114220.55555724
CCGCTTA450.003807114220.55555725
AAAAAGA450.003807114220.55555734
TATACAC2650.020.2452813
GTGGCTG759.15831E-619.73333428
TTATACA2750.019.5090922
ATGGGAG609.174411E-418.55
CGTCTTC609.174411E-418.537
GCTTATT500.007000837518.527
CAGGCCA609.174411E-418.54
Produced by FastQC (version 0.11.5)