##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630676.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125418
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89287821524821	33.0	31.0	34.0	30.0	34.0
2	31.989698448388587	33.0	31.0	34.0	30.0	34.0
3	31.989531008308216	33.0	31.0	34.0	30.0	34.0
4	35.66911448117495	37.0	35.0	37.0	33.0	37.0
5	35.67709579167265	37.0	35.0	37.0	35.0	37.0
6	35.77767146661564	37.0	35.0	37.0	35.0	37.0
7	35.71790333126027	37.0	35.0	37.0	33.0	37.0
8	35.777105359677236	37.0	35.0	37.0	35.0	37.0
9	37.412676011417815	39.0	37.0	39.0	34.0	39.0
10	37.34284552456585	39.0	37.0	39.0	34.0	39.0
11	37.46884817171379	39.0	37.0	39.0	35.0	39.0
12	37.435599355754356	39.0	37.0	39.0	35.0	39.0
13	37.50232821445088	39.0	37.0	39.0	35.0	39.0
14	38.5957039659379	40.0	38.0	41.0	34.0	41.0
15	38.537187644516735	40.0	38.0	41.0	34.0	41.0
16	38.382074343395686	40.0	38.0	41.0	34.0	41.0
17	38.4887655679408	40.0	38.0	41.0	34.0	41.0
18	38.59630196622494	40.0	38.0	41.0	34.0	41.0
19	38.64686887049706	40.0	38.0	41.0	34.0	41.0
20	38.65996906345182	40.0	38.0	41.0	34.0	41.0
21	38.568156086048255	40.0	38.0	41.0	34.0	41.0
22	38.492473169720455	40.0	38.0	41.0	34.0	41.0
23	38.42221212266182	40.0	38.0	41.0	34.0	41.0
24	38.333771866877164	40.0	38.0	41.0	34.0	41.0
25	38.23319619193417	40.0	37.0	41.0	34.0	41.0
26	37.88260058364828	40.0	37.0	41.0	33.0	41.0
27	37.647682150887434	40.0	36.0	41.0	33.0	41.0
28	37.49212234288539	40.0	35.0	41.0	33.0	41.0
29	37.28368336283468	39.0	35.0	41.0	32.0	41.0
30	37.04945063706964	39.0	35.0	41.0	32.0	41.0
31	36.70753001961441	39.0	35.0	41.0	31.0	41.0
32	36.43728970323239	38.0	35.0	40.0	31.0	41.0
33	36.19355275957199	38.0	35.0	40.0	30.0	41.0
34	35.9571273660878	38.0	35.0	40.0	30.0	41.0
35	35.71415586279481	38.0	35.0	40.0	30.0	41.0
36	35.45679248592706	38.0	35.0	40.0	28.0	41.0
37	34.99994418663988	38.0	35.0	40.0	25.0	41.0
38	34.647562550830024	37.0	34.0	40.0	23.0	41.0
39	34.295531741855235	37.0	33.0	40.0	21.0	41.0
40	33.84267011114832	37.0	33.0	40.0	18.0	41.0
41	33.432673141016444	36.0	33.0	40.0	15.0	41.0
42	33.02679838619656	36.0	33.0	40.0	12.0	41.0
43	31.973137827106157	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	5.0
18	3.0
19	13.0
20	43.0
21	71.0
22	128.0
23	238.0
24	347.0
25	647.0
26	886.0
27	1305.0
28	1854.0
29	2529.0
30	3088.0
31	3768.0
32	4574.0
33	5548.0
34	7015.0
35	8848.0
36	12003.0
37	20494.0
38	24592.0
39	27414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.917651373805995	18.83541437433223	14.198918815481033	21.048015436380744
2	18.47183019981183	22.26953068937473	33.177853258702896	26.080785852110544
3	23.315632524836946	24.876014607153678	30.846449472962412	20.961903395046964
4	14.245164171012133	18.131368703056978	29.1879953435711	38.435471782359784
5	12.817139485560286	39.72316573378622	30.980401537259404	16.479293243394093
6	31.254684335581818	29.289256725509894	18.07316334178507	21.38289559712322
7	25.64464430942927	34.58116059895709	19.729225470028226	20.044969621585416
8	33.141175907764435	28.724744454544005	19.273150584445613	18.860929053245947
9	27.44103717169784	11.838811015962621	21.09665279306001	39.62349901927953
10	21.71618109043359	28.05498413305905	25.395876190020573	24.83295858648679
11	29.59224353761023	23.082013746033265	19.13042784927203	28.19531486708447
12	21.248146199110177	30.15675580857612	26.61579677558245	21.97930121673125
13	32.53440494984771	17.56924843323925	28.691256438469757	21.205090178443285
14	23.802803425345644	22.613978854709853	28.37870162177678	25.20451609816773
15	30.60007335470188	21.983287885311515	25.63906297341689	21.77757578656971
16	24.181536940471066	23.004672375576074	30.5139613133681	22.299829370584764
17	19.26756924843324	30.218947838428296	28.389864293801526	22.123618619336938
18	21.14688481717138	20.363903108006827	32.80948508188617	25.679726992935624
19	21.72814109617439	25.725972348466726	34.01106699197882	18.534819563380054
20	22.380360075906168	20.481908497982744	35.955763925433345	21.181967500677736
21	24.76996922291856	21.379706262259006	33.62356280597682	20.22676170884562
22	23.113109760959354	22.671386882265704	33.00562917603534	21.209874180739607
23	21.351799582197135	22.62992552903092	34.04694700920123	21.971327879570715
24	21.197914174998804	23.7246647211724	33.95684829928719	21.120572804541613
25	19.57613739654595	24.421534389003174	34.180899073498225	21.821429140952656
26	22.535840150536607	23.247859158972396	34.08681369500391	20.129486995487092
27	19.622382752077055	25.27787080004465	33.358848012247044	21.740898435631248
28	18.821859701159322	24.145656923248655	35.88081455612432	21.1516688194677
29	19.92058556188107	24.40239837981789	34.455979205536686	21.221036852764357
30	19.021193130172705	25.255545455995154	37.018609768932684	18.704651644899457
31	18.47661420210815	26.61579677558245	33.551005437815945	21.356583584493453
32	18.115422028735907	26.476263375273085	34.8570380647116	20.551276531279402
33	17.829179224672696	25.278668133760707	37.46591398363871	19.42623865792789
34	18.572294248034574	27.075060996029276	32.55752762761326	21.79511712832289
35	18.112232693871693	26.26895660909917	34.90647275510692	20.71233794192221
36	18.486979540416844	26.819914206892154	33.40908003635842	21.28402621633258
37	18.227846082699454	27.69139995853865	32.99526383772664	21.085490121035257
38	18.417611507120192	25.426972204946658	34.217576424436686	21.937839863496468
39	20.086430974820203	25.86072174647977	32.63805833293467	21.414788945765363
40	17.70399783125229	25.492353569663045	35.17039021512064	21.633258383964023
41	17.799677877178716	26.395732669951684	32.231418137747376	23.57317131512223
42	17.90492592769778	25.39109218772425	34.672853976303244	22.031127908274726
43	16.727264029086733	26.7058954854965	33.97279497360826	22.59404551180851
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	28.0
2	47.0
3	173.0
4	299.0
5	299.0
6	492.5
7	686.0
8	707.5
9	729.0
10	1022.0
11	1315.0
12	1315.0
13	2438.5
14	3562.0
15	5004.5
16	6447.0
17	5788.0
18	5129.0
19	5129.0
20	5795.0
21	6461.0
22	4519.5
23	2578.0
24	2258.0
25	1938.0
26	1938.0
27	2002.0
28	2066.0
29	2112.5
30	2159.0
31	2270.5
32	2382.0
33	2382.0
34	2402.5
35	2423.0
36	2490.0
37	2557.0
38	2883.5
39	3210.0
40	3210.0
41	3591.5
42	3973.0
43	4418.5
44	4864.0
45	5673.5
46	6483.0
47	6483.0
48	7972.0
49	9461.0
50	9149.5
51	8838.0
52	8627.0
53	8416.0
54	8416.0
55	7073.0
56	5730.0
57	6615.5
58	7501.0
59	6813.5
60	6126.0
61	6126.0
62	5163.0
63	4200.0
64	4017.0
65	3834.0
66	3584.5
67	3335.0
68	3335.0
69	3167.5
70	3000.0
71	2619.0
72	2238.0
73	1908.0
74	1578.0
75	1578.0
76	1259.0
77	940.0
78	726.5
79	513.0
80	377.5
81	242.0
82	242.0
83	167.5
84	93.0
85	66.0
86	39.0
87	26.0
88	13.0
89	13.0
90	7.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	125418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.991962876142175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0467646388699	47.05943325519463
2	9.337263613579402	10.456234352325824
3	2.5618022328548644	4.303210065540831
4	1.1833561175666438	2.6503372721618907
5	0.6465026201868307	1.8099475354414838
6	0.40299612668033724	1.353872649858872
7	0.29904306220095694	1.17208056259867
8	0.21787423103212578	0.9759364684495049
9	0.15521758942811575	0.7821843754485003
>10	1.032410571884256	10.719354478623483
>50	0.06408065618591935	2.3991771516050324
>100	0.03987240829346093	4.547991516369261
>500	0.0042720437457279565	1.5922754309588734
>1k	0.008544087491455913	10.177964885423146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3549	2.829737358273932	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2607	2.078648997751519	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2211	1.7629048461943262	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	1580	1.2597872713645568	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1462	1.1657018928702418	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	1356	1.0811845189685692	RNA PCR Primer, Index 27 (95% over 23bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	785	0.6259069671020109	RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	619	0.49354957023712703	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	593	0.4728188936197356	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	466	0.3715575116809389	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	423	0.3372721618906377	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	395	0.3149468178411392	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	357	0.28464813663110555	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.28145880176689153	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	334	0.2663094611618747	RNA PCR Primer, Index 27 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	257	0.20491476502575387	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.19534676043311167	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.16903474780334563	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	209	0.16664274665518505	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	191	0.15229073976622176	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	190	0.15149340605016823	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	164	0.13076272943277678	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	162	0.12916806200066977	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	157	0.12518139342040216	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	149	0.11880272369197405	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	146	0.11641072254381349	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	139	0.11082938653143887	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	129	0.1028560493709037	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	127	0.10126138193879666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.002392001148160551	0.0
3	0.0	0.0	0.0	0.004784002296321102	0.0
4	0.0	0.0	0.0	0.006378669728428136	0.0
5	0.0	0.0	0.0	0.00797333716053517	0.0
6	0.0	0.0	0.0	0.00797333716053517	0.0
7	0.0	0.0	0.0	0.008770670876588687	0.0
8	0.0	0.0	0.0	0.009568004592642204	0.0
9	7.97333716053517E-4	0.0	0.0	0.015149340605016824	0.0
10	7.97333716053517E-4	0.0	0.0	0.01913600918528441	0.0
11	7.97333716053517E-4	0.0	0.0	0.020730676617391443	0.0
12	7.97333716053517E-4	7.97333716053517E-4	0.0	0.023122677765551995	0.0
13	7.97333716053517E-4	7.97333716053517E-4	0.0	0.02392001148160551	0.0
14	7.97333716053517E-4	7.97333716053517E-4	0.0	0.030298681210033648	0.0
15	7.97333716053517E-4	7.97333716053517E-4	0.0	0.035082683506354746	0.0
16	7.97333716053517E-4	7.97333716053517E-4	0.0	0.04784002296321102	0.0
17	7.97333716053517E-4	7.97333716053517E-4	0.0	0.06298936356822785	0.0
18	7.97333716053517E-4	7.97333716053517E-4	0.0	0.07494936930903061	0.0
19	7.97333716053517E-4	7.97333716053517E-4	0.0	0.08690937504983336	0.0
20	7.97333716053517E-4	7.97333716053517E-4	0.0	0.0932880447782615	0.0
21	7.97333716053517E-4	7.97333716053517E-4	0.0	0.1124240539635459	0.0
22	7.97333716053517E-4	7.97333716053517E-4	0.0	0.14670940375384714	0.0
23	7.97333716053517E-4	7.97333716053517E-4	0.0	0.19534676043311167	0.0
24	7.97333716053517E-4	7.97333716053517E-4	0.0	0.28066146805083797	0.0
25	7.97333716053517E-4	7.97333716053517E-4	0.0	0.2942161412237478	0.0
26	7.97333716053517E-4	7.97333716053517E-4	0.0	0.3340828270264236	0.0
27	7.97333716053517E-4	7.97333716053517E-4	0.0	0.3739495128290995	0.0
28	7.97333716053517E-4	7.97333716053517E-4	0.0	0.4305602066688992	0.0
29	7.97333716053517E-4	7.97333716053517E-4	0.0	0.5031175748297693	0.0
30	7.97333716053517E-4	7.97333716053517E-4	0.0	0.6498269785836164	0.0
31	7.97333716053517E-4	7.97333716053517E-4	0.0	1.0213844902645552	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGAGCA	20	0.0018356902	37.0	7
GCACAGG	20	0.0018356902	37.0	1
ATGCCCC	20	0.0018356902	37.0	37
AGTGGCT	55	6.1872925E-7	26.90909	27
AGGACCG	35	8.8252197E-4	26.428572	5
CCGTCTT	200	0.0	25.900002	37
CAGTGGC	55	1.8851952E-5	23.545454	26
TGCGTCT	40	0.0019216599	23.125002	35
GCCGTCT	225	0.0	23.022223	36
TGGCTGT	70	5.042377E-6	21.142859	29
TCCGCTT	45	0.0038071142	20.555557	24
CCGCTTA	45	0.0038071142	20.555557	25
AAAAAGA	45	0.0038071142	20.555557	34
TATACAC	265	0.0	20.245281	3
GTGGCTG	75	9.15831E-6	19.733334	28
TTATACA	275	0.0	19.509092	2
ATGGGAG	60	9.174411E-4	18.5	5
CGTCTTC	60	9.174411E-4	18.5	37
GCTTATT	50	0.0070008375	18.5	27
CAGGCCA	60	9.174411E-4	18.5	4
>>END_MODULE