FastQCFastQC Report
Fri 10 Feb 2017
ERR1630675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593707
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79030.49588788905363407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70760.44399629291958936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62010.38909285081887696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.2737015022209227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTA250.005496974629.59999834
ATCTCGC3500.017.97142811
CCGCTTA953.6081565E-617.52631625
TCCTCGG4200.016.73809420
AGTCGGT3000.016.6511
CGGCCTT3800.016.55263124
CGTATAT1352.220986E-816.44444310
CGTTATT1352.220986E-816.4444432
TACCGTC1708.54925E-1116.323537
GGTATCA39900.016.228071
GCGTTAT1504.6748028E-916.0333331
ATAGCGT1403.475543E-815.8571426
GTAAGGC700.002593083515.8571423
GCGTATA1403.475543E-815.8571429
CGCTTAT1059.347554E-615.85714226
TTTCGGA3400.015.77941230
TAGCGTA1302.5906047E-715.6538467
TCGGTGA3200.015.60937513
ATACCGT1902.7284841E-1115.5789486
GCAGTCG3250.015.369239
Produced by FastQC (version 0.11.5)