##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630675.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1593707
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01360036694324	33.0	31.0	34.0	30.0	34.0
2	32.17285611470616	33.0	31.0	34.0	30.0	34.0
3	32.271843569740234	34.0	31.0	34.0	30.0	34.0
4	35.860101637252015	37.0	35.0	37.0	35.0	37.0
5	35.836931757217606	37.0	35.0	37.0	35.0	37.0
6	35.8785454289904	37.0	35.0	37.0	35.0	37.0
7	35.85386460622938	37.0	35.0	37.0	35.0	37.0
8	35.85373534783997	37.0	35.0	37.0	35.0	37.0
9	37.57878204713916	39.0	37.0	39.0	35.0	39.0
10	37.49398979862672	39.0	37.0	39.0	35.0	39.0
11	37.60302740717083	39.0	37.0	39.0	35.0	39.0
12	37.536782482601886	39.0	37.0	39.0	35.0	39.0
13	37.58157553427324	39.0	37.0	39.0	35.0	39.0
14	38.852041184483724	40.0	38.0	41.0	35.0	41.0
15	38.82979493721243	40.0	38.0	41.0	35.0	41.0
16	38.80078082106686	40.0	38.0	41.0	35.0	41.0
17	38.80631132322315	40.0	38.0	41.0	35.0	41.0
18	38.79690808912805	40.0	38.0	41.0	35.0	41.0
19	38.84316690583652	40.0	38.0	41.0	35.0	41.0
20	38.82142012302136	40.0	38.0	41.0	35.0	41.0
21	38.752926980931875	40.0	38.0	41.0	35.0	41.0
22	38.69848912002018	40.0	38.0	41.0	34.0	41.0
23	38.674603299100774	40.0	38.0	41.0	34.0	41.0
24	38.61720127978355	40.0	38.0	41.0	34.0	41.0
25	38.573380175904354	40.0	38.0	41.0	34.0	41.0
26	38.38246114248102	40.0	38.0	41.0	34.0	41.0
27	38.26666821442085	40.0	38.0	41.0	34.0	41.0
28	38.176261383052214	40.0	38.0	41.0	33.0	41.0
29	38.07259929209071	40.0	37.0	41.0	33.0	41.0
30	37.986520734363346	40.0	37.0	41.0	33.0	41.0
31	37.92958617863886	40.0	37.0	41.0	33.0	41.0
32	37.821970412378185	40.0	37.0	41.0	33.0	41.0
33	37.77591803261202	40.0	37.0	41.0	33.0	41.0
34	37.69092875917593	40.0	37.0	41.0	33.0	41.0
35	37.62292002231276	40.0	37.0	41.0	33.0	41.0
36	37.56993161227252	40.0	37.0	41.0	33.0	41.0
37	37.37765913056791	40.0	36.0	41.0	32.0	41.0
38	37.26006097733147	40.0	36.0	41.0	31.0	41.0
39	37.17827492757451	40.0	36.0	41.0	31.0	41.0
40	37.060420767430905	40.0	36.0	41.0	31.0	41.0
41	36.91854023355611	39.0	36.0	41.0	31.0	41.0
42	36.81142644162321	39.0	35.0	41.0	31.0	41.0
43	35.98175386065318	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	11.0
11	4.0
12	7.0
13	1.0
14	2.0
15	8.0
16	6.0
17	26.0
18	51.0
19	100.0
20	216.0
21	435.0
22	724.0
23	1332.0
24	2221.0
25	3630.0
26	5526.0
27	8295.0
28	11802.0
29	16671.0
30	22892.0
31	30053.0
32	39004.0
33	50665.0
34	67443.0
35	90207.0
36	127504.0
37	197124.0
38	348526.0
39	569219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31687066694192	20.030595335278065	12.631117263085374	24.021416734694647
2	18.221793591921227	21.867633134572415	35.47421201011227	24.436361263394087
3	20.408456510512913	24.40090932649477	29.913403153779207	25.277231009213114
4	14.429691279513737	16.0245892124462	34.57429753398837	34.971421974051694
5	13.796387918230893	37.904206984094316	34.19976193867505	14.099643158999742
6	33.751373370387405	34.831245643019706	16.312345995844908	15.105034990747987
7	27.70352392252779	31.5611338847103	22.138699271572506	18.596642921189403
8	26.617063236843407	33.79309998638394	20.75657570682691	18.83326106994573
9	26.344365683278042	14.259961209933822	20.973365869636012	38.422307237152125
10	17.09046895069169	27.520617026843702	33.10407747471775	22.284836547746856
11	33.98115211892776	22.654352399782393	22.082038919324567	21.28245656196528
12	20.93138826647558	26.862967910663627	29.609959672637444	22.595684150223345
13	28.951432101383755	20.64902770710049	26.720909175902474	23.678631015613284
14	22.512168171439292	22.190779108079465	26.264300777997462	29.03275194248378
15	25.19208361386378	28.178705370560586	23.92221405816753	22.70699695740811
16	24.228669385275964	27.24860968797903	25.44645910446525	23.076261822279754
17	22.600954880665018	27.50649899887495	26.624906585714943	23.267639534745093
18	22.037488697734275	25.81214740225148	28.68915051511978	23.461213384894464
19	22.958674335997774	26.44501153599752	28.56698251309682	22.029331614907885
20	23.576416493119503	25.406865879361767	28.622450676316287	22.39426695120245
21	23.832235159913335	26.247547384807874	27.457180021170768	22.463037434108028
22	23.55909837881116	25.92433866450985	27.735901266669472	22.780661690009516
23	22.892601965104	26.17312969071479	27.938573401509814	22.995694942671392
24	23.12796517804088	26.694618270485105	27.756293973735445	22.42112257773857
25	23.23764656866036	26.021784430889745	27.820044713363245	22.920524287086646
26	22.899504112110947	26.791687556119157	27.780075007513926	22.528733324255963
27	23.363077403813875	26.132093289418947	27.322713648117254	23.182115658649927
28	22.384541198601752	26.455490250089888	28.356592522966896	22.80337602834147
29	22.200504860680162	26.858199154549737	28.25105242055158	22.69024356421852
30	22.086242954319708	27.48654551934578	28.195709750914066	22.23150177542045
31	22.749162800941452	27.005214885797702	27.667444517718753	22.578177795542093
32	21.94054490568216	26.87595649639488	27.805926685394493	23.377571912528463
33	21.990993325623844	26.563916704889923	28.332309514860636	23.1127804546256
34	22.753304089145622	26.33815375097179	27.95300516343343	22.95553699644916
35	22.665333088202537	26.453796086733632	28.33607432231897	22.54479650274486
36	22.081662438578736	26.87419958624766	27.957271945219542	23.086866029954063
37	22.810842896467168	25.803049117560505	28.05685110249249	23.329256883479836
38	22.101176690571105	25.873137283076503	28.80836941796704	23.217316608385357
39	22.278624615440602	25.818171094184816	28.701888113687147	23.201316176687435
40	21.81542780448351	25.67975167330005	29.363615771280415	23.141204750936026
41	21.15156675599718	26.044185035266832	29.701569987456917	23.102678221279067
42	21.865311503306444	25.63802505730351	29.411052345255435	23.085611094134617
43	21.03648914135409	25.484420913003458	29.38018092409709	24.098909021545364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	526.5
2	422.0
3	1008.0
4	1594.0
5	1594.0
6	2198.0
7	2802.0
8	2752.5
9	2703.0
10	4005.5
11	5308.0
12	5308.0
13	8768.5
14	12229.0
15	16405.0
16	20581.0
17	20242.5
18	19904.0
19	19904.0
20	22503.5
21	25103.0
22	24023.0
23	22943.0
24	25433.0
25	27923.0
26	27923.0
27	30801.5
28	33680.0
29	40241.0
30	46802.0
31	50857.5
32	54913.0
33	54913.0
34	59665.5
35	64418.0
36	69243.0
37	74068.0
38	81872.0
39	89676.0
40	89676.0
41	93958.0
42	98240.0
43	95740.0
44	93240.0
45	97153.0
46	101066.0
47	101066.0
48	104028.5
49	106991.0
50	107952.0
51	108913.0
52	112249.0
53	115585.0
54	115585.0
55	106349.5
56	97114.0
57	91379.5
58	85645.0
59	78671.0
60	71697.0
61	71697.0
62	66260.5
63	60824.0
64	54062.0
65	47300.0
66	40948.5
67	34597.0
68	34597.0
69	29539.5
70	24482.0
71	21116.0
72	17750.0
73	13923.5
74	10097.0
75	10097.0
76	7890.0
77	5683.0
78	4587.5
79	3492.0
80	2815.0
81	2138.0
82	2138.0
83	1639.0
84	1140.0
85	1013.5
86	887.0
87	806.0
88	725.0
89	725.0
90	536.0
91	347.0
92	190.5
93	34.0
94	24.5
95	15.0
96	15.0
97	9.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1593707.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.39642235752625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.47068301931334	50.20504266477808
2	7.58980605487138	8.712554278742154
3	1.9285476731830242	3.32075210359912
4	0.80960274369247	1.858732040751403
5	0.4635088448337178	1.330187471226258
6	0.2931085063231393	1.0094027775303933
7	0.19727338058730884	0.7925950390460339
8	0.14485369903017578	0.6651267271668766
9	0.11579659776598081	0.5981679389646734
>10	0.7400891336068156	8.52193067208829
>50	0.11662924501406863	4.724357994654238
>100	0.1213327978719129	13.296021447740735
>500	0.0073434545217389196	2.7146344660041932
>1k	0.0010960379883192418	0.9180889737871987
>5k	3.2881139649577255E-4	1.3324054039203006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7903	0.49588788905363407	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7076	0.44399629291958936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6201	0.38909285081887696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4362	0.2737015022209227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.27467909722427E-5	0.0	4.392275368056989E-4	0.0
3	0.0	6.27467909722427E-5	0.0	6.902147006946697E-4	0.0
4	0.0	6.27467909722427E-5	0.0	0.0011921890284726114	0.0
5	0.0	2.509871638889708E-4	0.0	0.0013176826104170968	0.0
6	0.0	2.509871638889708E-4	0.0	0.0013176826104170968	0.0
7	0.0	2.509871638889708E-4	0.0	0.0015686697743060676	0.0
8	0.0	2.509871638889708E-4	0.0	0.001694163356250553	0.0
9	0.0	2.509871638889708E-4	0.0	0.0030745927576398924	0.0
10	0.0	2.509871638889708E-4	0.0	0.0051452368597239015	0.0
11	0.0	2.509871638889708E-4	0.0	0.00627467909722427	0.0
12	0.0	2.509871638889708E-4	0.0	0.007717855289585853	0.0
13	0.0	2.509871638889708E-4	0.0	0.008219829617363793	0.0
14	0.0	2.509871638889708E-4	0.0	0.009412018645836405	0.0
15	0.0	2.509871638889708E-4	0.0	0.011545409538892657	0.0
16	0.0	2.509871638889708E-4	0.0	0.01556120416111619	0.0
17	0.0	2.509871638889708E-4	0.0	0.02089468139375682	0.0
18	0.0	2.509871638889708E-4	0.0	0.02340455303264653	0.0
19	0.0	2.509871638889708E-4	0.0	0.026353652208341936	0.0
20	0.0	2.509871638889708E-4	0.0	0.028989017429176128	0.0
21	0.0	2.509871638889708E-4	0.0	0.03451073503473349	0.0
22	0.0	2.509871638889708E-4	0.0	0.0451149427090425	0.0
23	0.0	3.137339548612135E-4	0.0	0.06400172679168756	0.0
24	0.0	3.137339548612135E-4	0.0	0.09041812579100174	0.0
25	0.0	3.137339548612135E-4	0.0	0.09832422145350432	0.0
26	0.0	3.137339548612135E-4	0.0	0.11200302188545322	0.0
27	0.0	3.137339548612135E-4	0.0	0.13333693081601575	0.0
28	0.0	3.764807458334562E-4	0.0	0.17832637994311376	0.0
29	0.0	3.764807458334562E-4	0.0	0.23944175435007814	0.0
30	0.0	3.764807458334562E-4	0.0	0.3344403958820536	0.0
31	0.0	3.764807458334562E-4	0.0	0.6224481664446476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCTA	25	0.0054969746	29.599998	34
ATCTCGC	350	0.0	17.971428	11
CCGCTTA	95	3.6081565E-6	17.526316	25
TCCTCGG	420	0.0	16.738094	20
AGTCGGT	300	0.0	16.65	11
CGGCCTT	380	0.0	16.552631	24
CGTATAT	135	2.220986E-8	16.444443	10
CGTTATT	135	2.220986E-8	16.444443	2
TACCGTC	170	8.54925E-11	16.32353	7
GGTATCA	3990	0.0	16.22807	1
GCGTTAT	150	4.6748028E-9	16.033333	1
ATAGCGT	140	3.475543E-8	15.857142	6
GTAAGGC	70	0.0025930835	15.857142	3
GCGTATA	140	3.475543E-8	15.857142	9
CGCTTAT	105	9.347554E-6	15.857142	26
TTTCGGA	340	0.0	15.779412	30
TAGCGTA	130	2.5906047E-7	15.653846	7
TCGGTGA	320	0.0	15.609375	13
ATACCGT	190	2.7284841E-11	15.578948	6
GCAGTCG	325	0.0	15.36923	9
>>END_MODULE