FastQCFastQC Report
Fri 10 Feb 2017
ERR1630674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316436
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15570.49204262473296334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.42978674992731547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10040.31728374774045937No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.24143902716505075No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA7140.22563804371184062No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA6550.20699288323705267No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA6470.20446472588453907No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA5670.17918315235940285No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5180.16369818857525692No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT4240.13399233968322188No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG4060.12830398564006623No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT3870.12229961192784639No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG3630.11471513987030553No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG3470.10965882516527829No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC3470.10965882516527829No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG3410.10776270715089308No Hit
TAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGG3300.10428649079118685No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG3180.10049425476241641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTG250.005490429.5999985
GGTATCA3550.029.1830981
CTCGAAA400.001928112423.12533
AAGACGG1005.293259E-1022.1999995
ACGGACC1059.749783E-1021.1428578
CGGACCA1059.749783E-1021.1428579
CCTCGAA450.003819773720.55555732
TATAGTA450.003819773720.5555572
CGTCAGA1001.2809323E-820.3499981
AAGACTG555.131779E-420.1818185
GCCTCGA555.131779E-420.18181831
ATAAGAC555.131779E-420.1818183
CAAGACG1101.7407729E-920.1818184
GACGGAC1153.0249794E-919.3043487
GTATCAA5500.018.8363632
AGCCGCT609.216662E-418.518
TCTTATA1205.131369E-918.537
TAAACTG701.21565696E-418.55
GTCAGAT1205.131369E-918.52
AGACGGA1205.131369E-918.56
Produced by FastQC (version 0.11.5)