FastQCFastQC Report
Fri 10 Feb 2017
ERR1630673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962921
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70920.3612982896407956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66000.33623360288060494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56130.2859513959043691No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.19445510033261654No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA21250.10825703123049782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG609.2399894E-418.51
TCTAGAC3550.018.2394373
AAGACGG5000.017.765
GTTCTAG3350.017.6716421
GACGGAC5000.017.027
CAAGACG5550.017.0000024
ACGGACC5200.016.3653858
AGTCGGT2600.016.36538511
GCGCAAG5450.016.2935771
GGTATCA32700.016.0107021
CTAGACA3700.016.04
GCAGTCG2800.015.8571429
TTAACGG700.002593370615.85714235
CGCAAGA5500.015.8090922
GTATAGA1302.591496E-715.6538461
GTCTAGA3200.015.6093761
AGACGGA5850.014.8632476
CGAACGA2750.014.816
CTTATAC11450.014.70305737
TCTTATA18600.014.62096837
Produced by FastQC (version 0.11.5)