##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630673.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1962921
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07993291630178	33.0	31.0	34.0	30.0	34.0
2	32.24289158860698	33.0	31.0	34.0	30.0	34.0
3	32.35284609008717	34.0	31.0	34.0	30.0	34.0
4	35.91931004864689	37.0	35.0	37.0	35.0	37.0
5	35.89396924277646	37.0	35.0	37.0	35.0	37.0
6	35.93611459656298	37.0	35.0	37.0	35.0	37.0
7	35.911667866409296	37.0	35.0	37.0	35.0	37.0
8	35.912991404136996	37.0	35.0	37.0	35.0	37.0
9	37.654588748095314	39.0	37.0	39.0	35.0	39.0
10	37.560462188748296	39.0	37.0	39.0	35.0	39.0
11	37.66657955159683	39.0	37.0	39.0	35.0	39.0
12	37.592201621970524	39.0	37.0	39.0	35.0	39.0
13	37.645933280045405	39.0	37.0	39.0	35.0	39.0
14	38.93276295887608	40.0	38.0	41.0	36.0	41.0
15	38.90691576482192	40.0	38.0	41.0	36.0	41.0
16	38.892080730706944	40.0	38.0	41.0	35.0	41.0
17	38.88170639572352	40.0	38.0	41.0	35.0	41.0
18	38.87633175252596	40.0	38.0	41.0	35.0	41.0
19	38.90150851715377	40.0	38.0	41.0	35.0	41.0
20	38.88283481607258	40.0	38.0	41.0	35.0	41.0
21	38.81279328103373	40.0	38.0	41.0	35.0	41.0
22	38.756372773025504	40.0	38.0	41.0	35.0	41.0
23	38.72715560126974	40.0	38.0	41.0	35.0	41.0
24	38.67739608471253	40.0	38.0	41.0	34.0	41.0
25	38.63802312981521	40.0	38.0	41.0	34.0	41.0
26	38.46600601858149	40.0	38.0	41.0	34.0	41.0
27	38.35933437973306	40.0	38.0	41.0	34.0	41.0
28	38.25969104207454	40.0	38.0	41.0	34.0	41.0
29	38.15919591262206	40.0	38.0	41.0	33.0	41.0
30	38.083586654786416	40.0	38.0	41.0	33.0	41.0
31	38.03886045337535	40.0	37.0	41.0	33.0	41.0
32	37.93798273083838	40.0	37.0	41.0	33.0	41.0
33	37.89169406206363	40.0	37.0	41.0	33.0	41.0
34	37.819294306800934	40.0	37.0	41.0	33.0	41.0
35	37.76764118372568	40.0	37.0	41.0	33.0	41.0
36	37.72016347066438	40.0	37.0	41.0	33.0	41.0
37	37.54028256868208	40.0	37.0	41.0	33.0	41.0
38	37.43029240606219	40.0	36.0	41.0	32.0	41.0
39	37.36763119860657	40.0	36.0	41.0	32.0	41.0
40	37.259708363199536	40.0	36.0	41.0	32.0	41.0
41	37.13167875834025	40.0	36.0	41.0	31.0	41.0
42	37.032894344703635	39.0	36.0	41.0	31.0	41.0
43	36.19963411670668	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	7.0
11	7.0
12	4.0
13	0.0
14	3.0
15	8.0
16	9.0
17	13.0
18	48.0
19	101.0
20	220.0
21	412.0
22	721.0
23	1401.0
24	2363.0
25	3722.0
26	5925.0
27	8815.0
28	12964.0
29	18432.0
30	25503.0
31	33868.0
32	44480.0
33	59219.0
34	80242.0
35	109557.0
36	155928.0
37	244068.0
38	442964.0
39	711909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.009514392071814	19.130927836627148	11.92508511549879	24.934472655802246
2	19.084772132958992	20.933700337405327	34.209680369204875	25.771847160430806
3	20.592983619819645	23.27887877301226	28.940696034124652	27.18744157304344
4	14.327779874992421	15.313453776285444	34.27218925264949	36.08657709607264
5	14.665083312064011	36.95395790253403	33.61434311416506	14.766615671236897
6	35.25185170467889	34.30438616734958	15.619630132847934	14.824131995123594
7	29.221196370103534	30.36138489526578	21.354858397255928	19.06256033737476
8	27.688582474791396	32.56524332869229	19.91022562803088	19.83594856848544
9	26.77499502017656	13.504873604184784	20.200711083125608	39.51942029251305
10	17.40177011708571	26.279610845265804	32.538344640461844	23.78027439718664
11	36.018719041673094	21.55063805420595	21.456594534369952	20.974048369751
12	21.910764620685192	25.524104128490144	29.007382365362638	23.557748885462022
13	29.992954377685095	19.661871262266793	25.47015391857339	24.87502044147472
14	23.030830074159887	20.903286479690216	25.409020536231463	30.65686290991843
15	25.89508187033508	27.534628240260307	22.518685163590384	24.051604725814233
16	25.42226610240555	26.019335469945048	24.33597684267477	24.222421584974636
17	23.59712897258728	26.268148336076695	25.67194502478704	24.462777666548984
18	23.862091240554257	24.581478317262896	27.19813991495328	24.358290527229574
19	23.977989944577494	25.32323002301162	26.828537674211034	23.870242358199846
20	24.484123405883377	24.597831497039362	26.857728864279306	24.060316232797955
21	25.389254075940908	24.72076054003192	26.412575951859502	23.477409432167672
22	25.066979262028376	24.67215950107009	26.469582830893344	23.79127840600819
23	24.15614280961893	24.840276302510393	26.763074010619885	24.24050687725079
24	23.98568256185552	25.521047459373047	26.36866180554388	24.124608173227553
25	24.444437651846407	25.057605476735944	26.36667497061777	24.131281900799877
26	24.18441699895207	25.73292557367311	26.348284011429907	23.73437341594491
27	24.501036974997977	24.91959686609904	26.371463752234554	24.20790240666843
28	23.769576055276804	25.107734850256325	27.125849690333947	23.99683940413292
29	23.367573121893344	25.464193413795055	27.157384326725325	24.01084913758628
30	23.243166688827518	26.07308190192066	27.020649328220546	23.66310208103128
31	24.051757559270087	25.922744725844797	26.07410079162636	23.951396923258756
32	23.49778722628165	25.606634194651747	26.296167802983412	24.599410776083193
33	23.21081694067158	25.392005078146298	26.946881713527954	24.450296267654174
34	24.2709207349659	25.15414527635091	26.621448341527753	23.953485647155436
35	23.720924071829685	25.17584762708229	27.4714061340217	23.63182216706633
36	23.316577692123115	25.692424707871588	26.711569135996815	24.279428464008486
37	23.855264679526073	25.02444061681545	26.963082059848563	24.157212643809913
38	23.051666368641428	24.767476633038214	27.57059504687147	24.610261951448887
39	23.45438252481888	24.849038753979404	27.362843435879487	24.33373528532223
40	22.929603381898712	24.831462906556094	28.032916250832308	24.206017460712886
41	22.468046345217154	25.01578005431701	28.377963249667204	24.13821035079863
42	22.93877338925	25.027599174903116	28.289116067330273	23.744511368516612
43	22.24134338569917	24.37260592759464	28.310512751149947	25.075537935556248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	310.0
1	305.5
2	301.0
3	834.0
4	1367.0
5	1367.0
6	1858.5
7	2350.0
8	2238.5
9	2127.0
10	3067.0
11	4007.0
12	4007.0
13	6994.5
14	9982.0
15	13556.5
16	17131.0
17	16754.5
18	16378.0
19	16378.0
20	19305.5
21	22233.0
22	21148.0
23	20063.0
24	22782.5
25	25502.0
26	25502.0
27	29610.0
28	33718.0
29	38385.0
30	43052.0
31	48649.0
32	54246.0
33	54246.0
34	61744.0
35	69242.0
36	76639.5
37	84037.0
38	91546.0
39	99055.0
40	99055.0
41	105210.5
42	111366.0
43	115506.5
44	119647.0
45	123261.5
46	126876.0
47	126876.0
48	132542.5
49	138209.0
50	137433.5
51	136658.0
52	137937.0
53	139216.0
54	139216.0
55	135051.5
56	130887.0
57	124953.5
58	119020.0
59	113459.0
60	107898.0
61	107898.0
62	100544.0
63	93190.0
64	84260.5
65	75331.0
66	66271.0
67	57211.0
68	57211.0
69	48186.0
70	39161.0
71	33124.5
72	27088.0
73	20904.5
74	14721.0
75	14721.0
76	11413.0
77	8105.0
78	6531.5
79	4958.0
80	4245.5
81	3533.0
82	3533.0
83	2673.0
84	1813.0
85	1527.0
86	1241.0
87	1126.0
88	1011.0
89	1011.0
90	791.0
91	571.0
92	322.0
93	73.0
94	49.5
95	26.0
96	26.0
97	18.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1962921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.367115220830044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.34272728438845	50.66551523952165
2	8.903612908765208	10.571636268726676
3	2.299611942928981	4.09563981537185
4	1.0090402767569855	2.396152414907608
5	0.5477052034550766	1.625783896028285
6	0.35565880240948394	1.2668662261167756
7	0.24092610657464908	1.0012161550106222
8	0.17732175016655388	0.8421664618637634
9	0.1402652698058807	0.7494429990641879
>10	0.8115745384659518	9.371401059031715
>50	0.09209027639834742	3.7967309678425494
>100	0.07121963305294438	8.455384908883227
>500	0.005755025601245338	2.341895803198284
>1k	0.002233293516901176	1.835730147399783
>5k	2.576877134885972E-4	0.9844376370330511
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7092	0.3612982896407956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6600	0.33623360288060494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5613	0.2859513959043691	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3817	0.19445510033261654	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2125	0.10825703123049782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	5.094448528494015E-5	0.0	0.0	0.0
2	0.0	5.094448528494015E-5	0.0	5.603893381343416E-4	0.0
3	0.0	5.094448528494015E-5	0.0	9.679452204138628E-4	0.0
4	0.0	5.094448528494015E-5	0.0	0.0016811680144030248	0.0
5	0.0	1.018889705698803E-4	0.0	0.0018849459555427854	0.0
6	0.0	1.018889705698803E-4	0.0	0.0019358904408277257	0.0
7	0.0	1.018889705698803E-4	0.0	0.002394390808392187	0.0
8	0.0	1.018889705698803E-4	0.0	0.0026491132348168875	0.0
9	0.0	1.5283345585482045E-4	0.0	0.004890670587354254	0.0
10	0.0	1.5283345585482045E-4	0.0	0.007896395219165723	0.0
11	0.0	1.5283345585482045E-4	0.0	0.009068118380719347	0.0
12	0.0	1.5283345585482045E-4	0.0	0.009781341174708509	0.0
13	0.0	1.5283345585482045E-4	0.0	0.01054550845398261	5.094448528494015E-5
14	0.0	1.5283345585482045E-4	0.0	0.011411564703826593	5.094448528494015E-5
15	0.0	1.5283345585482045E-4	0.0	0.012379509924240455	5.094448528494015E-5
16	0.0	2.037779411397606E-4	0.0	0.014519178306207942	5.094448528494015E-5
17	0.0	2.037779411397606E-4	0.0	0.01701545808517001	5.094448528494015E-5
18	0.0	3.5661139699458104E-4	0.0	0.018951348525997735	5.094448528494015E-5
19	0.0	3.5661139699458104E-4	0.0	0.0211929058785351	5.094448528494015E-5
20	0.0	3.5661139699458104E-4	0.0	0.023892963598636928	5.094448528494015E-5
21	0.0	3.5661139699458104E-4	0.0	0.027713799995007442	5.094448528494015E-5
22	0.0	3.5661139699458104E-4	0.0	0.03321580440578098	5.094448528494015E-5
23	0.0	4.075558822795212E-4	0.0	0.040959366169091876	5.094448528494015E-5
24	0.0	4.075558822795212E-4	0.0	0.054052098887321494	5.094448528494015E-5
25	0.0	4.075558822795212E-4	0.0	0.057771046313122125	5.094448528494015E-5
26	0.0	4.075558822795212E-4	0.0	0.0659731084439975	1.018889705698803E-4
27	0.0	4.075558822795212E-4	0.0	0.0788111187358024	1.018889705698803E-4
28	0.0	4.585003675644613E-4	0.0	0.10840986468635264	1.018889705698803E-4
29	0.0	5.603893381343416E-4	0.0	0.152374955487256	1.018889705698803E-4
30	0.0	6.113338234192818E-4	0.0	0.2228311786363282	1.018889705698803E-4
31	0.0	6.113338234192818E-4	0.0	0.46384953851938004	1.018889705698803E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	60	9.2399894E-4	18.5	1
TCTAGAC	355	0.0	18.239437	3
AAGACGG	500	0.0	17.76	5
GTTCTAG	335	0.0	17.671642	1
GACGGAC	500	0.0	17.02	7
CAAGACG	555	0.0	17.000002	4
ACGGACC	520	0.0	16.365385	8
AGTCGGT	260	0.0	16.365385	11
GCGCAAG	545	0.0	16.293577	1
GGTATCA	3270	0.0	16.010702	1
CTAGACA	370	0.0	16.0	4
GCAGTCG	280	0.0	15.857142	9
TTAACGG	70	0.0025933706	15.857142	35
CGCAAGA	550	0.0	15.809092	2
GTATAGA	130	2.591496E-7	15.653846	1
GTCTAGA	320	0.0	15.609376	1
AGACGGA	585	0.0	14.863247	6
CGAACGA	275	0.0	14.8	16
CTTATAC	1145	0.0	14.703057	37
TCTTATA	1860	0.0	14.620968	37
>>END_MODULE