FastQCFastQC Report
Fri 10 Feb 2017
ERR1630672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020408
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32340.16006667960134785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.13492324322612065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20630.10210808905923952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.017.7448981
GACGGAC3950.015.924057
CGCAAGA4550.015.8571432
CGAATTA1059.350029E-615.85714215
AAGACGG4300.015.4883725
TAATTCG750.00410583814.85
CTTATAC8750.014.58857237
CGGACCA4450.014.5505639
TCTTATA13050.014.4597737
CAAGACG5200.014.2307694
TCTATAC1458.918814E-714.0344833
CGTTATT4100.013.9878052
ACGGACC4500.013.97777758
TTAACGG2009.840733E-1013.87500135
GCGCAAG5400.013.7037031
ACCGGAC950.001245645113.6315793
GCGTTAT4350.013.6091961
CTAGACT1652.5976624E-713.4545454
TAGACTG2355.638867E-1113.38297755
CTAGCGG3600.013.36111129
Produced by FastQC (version 0.11.5)