##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630672.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2020408
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06227455048683	33.0	31.0	34.0	30.0	34.0
2	32.23538661498073	33.0	31.0	34.0	30.0	34.0
3	32.34464177532459	34.0	31.0	34.0	30.0	34.0
4	35.915184952742216	37.0	35.0	37.0	35.0	37.0
5	35.88693026359032	37.0	35.0	37.0	35.0	37.0
6	35.921335690612985	37.0	35.0	37.0	35.0	37.0
7	35.8980354463059	37.0	35.0	37.0	35.0	37.0
8	35.88406698053066	37.0	35.0	37.0	35.0	37.0
9	37.64619126433869	39.0	37.0	39.0	35.0	39.0
10	37.54297844791745	39.0	37.0	39.0	35.0	39.0
11	37.649228274685115	39.0	37.0	39.0	35.0	39.0
12	37.57016305617479	39.0	37.0	39.0	35.0	39.0
13	37.62121017141092	39.0	37.0	39.0	35.0	39.0
14	38.91644261951051	40.0	38.0	41.0	36.0	41.0
15	38.89727470887068	40.0	38.0	41.0	36.0	41.0
16	38.87405266659012	40.0	38.0	41.0	35.0	41.0
17	38.853221230563335	40.0	38.0	41.0	35.0	41.0
18	38.84033125982475	40.0	38.0	41.0	35.0	41.0
19	38.85949273612062	40.0	38.0	41.0	35.0	41.0
20	38.83835888592799	40.0	38.0	41.0	35.0	41.0
21	38.76298302125115	40.0	38.0	41.0	35.0	41.0
22	38.71942894702456	40.0	38.0	41.0	35.0	41.0
23	38.69541696528622	40.0	38.0	41.0	34.0	41.0
24	38.64764196142561	40.0	38.0	41.0	34.0	41.0
25	38.607525806668754	40.0	38.0	41.0	34.0	41.0
26	38.441392035668045	40.0	38.0	41.0	34.0	41.0
27	38.340788593195036	40.0	38.0	41.0	34.0	41.0
28	38.24201151450598	40.0	38.0	41.0	34.0	41.0
29	38.15403819426571	40.0	38.0	41.0	33.0	41.0
30	38.088410360679624	40.0	38.0	41.0	33.0	41.0
31	38.05828080268936	40.0	38.0	41.0	33.0	41.0
32	37.97098506836243	40.0	37.0	41.0	33.0	41.0
33	37.94065456086098	40.0	37.0	41.0	33.0	41.0
34	37.88234554604813	40.0	37.0	41.0	33.0	41.0
35	37.83487246140383	40.0	37.0	41.0	33.0	41.0
36	37.79998346868553	40.0	37.0	41.0	33.0	41.0
37	37.63685404136194	40.0	37.0	41.0	33.0	41.0
38	37.53990085170916	40.0	37.0	41.0	32.0	41.0
39	37.49495992888565	40.0	37.0	41.0	32.0	41.0
40	37.40587544693943	40.0	36.0	41.0	32.0	41.0
41	37.299979014139716	40.0	36.0	41.0	32.0	41.0
42	37.220721260260305	39.0	36.0	41.0	31.0	41.0
43	36.35620330151138	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	3.0
13	6.0
14	3.0
15	3.0
16	7.0
17	19.0
18	37.0
19	98.0
20	228.0
21	455.0
22	891.0
23	1506.0
24	2449.0
25	3984.0
26	5982.0
27	9054.0
28	12971.0
29	18741.0
30	25425.0
31	34351.0
32	45553.0
33	60597.0
34	82224.0
35	111983.0
36	160703.0
37	245915.0
38	457900.0
39	739316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.950528804083135	18.33169340054088	11.610080736168142	26.10769705920784
2	19.85475210947492	19.946812722974766	33.53149462880764	26.66694053874267
3	20.7109158150235	22.087865421241652	28.603678068984085	28.597540694750762
4	14.842546653943165	14.800970893007747	33.34697744218	37.00950501086909
5	15.424805286852953	35.81900289446488	33.60697443288682	15.149217385795344
6	36.765296910327024	33.54594715522805	14.851950695107128	14.836805239337798
7	30.483595392613772	29.259931657368217	20.533228931978094	19.72324401803992
8	28.00469014179314	32.28610260897799	19.28778741719494	20.421419832033926
9	26.92673954963552	13.229902079184006	19.04714295330448	40.79621541787599
10	17.639753950687187	25.593790957074013	32.302089478956724	24.46436561328207
11	37.45233635978475	20.53119963888482	20.593117825706493	21.423346175623934
12	22.585883643303728	24.488073696005955	27.740981029574225	25.185061631116092
13	30.73567319076147	18.90058839600714	24.223028220042682	26.140710193188703
14	23.582415036962832	20.172608700816866	23.82271303617883	32.42226322604147
15	26.780184992338178	26.82581933946015	21.185671408943143	25.208324259258525
16	26.71663347205119	25.25044446468238	22.75109779806851	25.281824265197923
17	24.95926565327399	25.292317195338764	24.15522013375516	25.59319701763208
18	24.838299986933333	24.006933253085517	25.632446515753255	25.5223202442279
19	25.375072757581634	24.36017873617606	25.094139401546617	25.170609104695686
20	25.817161682194882	23.758567576449906	25.050534347517928	25.37373639383728
21	26.37640516172971	24.235896907951265	24.51806763782365	24.869630292495376
22	26.230741513595273	23.928434256843172	24.540142387082213	25.30068184247934
23	25.208571734026	24.102310028469496	24.979459594299765	25.70965864320474
24	25.44896872314899	24.720403007709336	24.482332281400588	25.34829598774109
25	25.874724313109038	24.117356494331837	24.521334304754287	25.48658488780484
26	25.64244449635915	24.795734326928027	24.582906026901497	24.978915149811325
27	26.08507786546084	23.917693851934857	24.30162620619202	25.69560207641229
28	24.978172725508905	24.237480746463092	25.35468083674189	25.429665691286118
29	24.76940301166893	24.65026865860757	25.265342445684237	25.314985884039267
30	24.733073715803936	25.159076780531457	25.151603042553784	24.956246461110826
31	25.47262731091938	24.89190302156792	24.313801964751676	25.321667702761026
32	24.806375741929354	24.48446056440085	24.544596932896724	26.164566760773074
33	24.557366630898315	24.26747468828078	25.336169724134926	25.838988956685977
34	25.66541015478062	23.992975676200054	24.93144948941006	25.410164679609267
35	25.344534371275508	24.164376700151653	25.5558283277437	24.93526060082914
36	24.567612086271684	24.871016151193224	24.997426262418283	25.56394550011681
37	25.36418386781284	23.962041330265965	25.278854567988247	25.39492023393295
38	24.547269660385425	23.833552430994136	26.018655637871163	25.600522270749277
39	24.813552510186064	23.65893423506539	25.83740511817415	25.690108136574395
40	24.479164604376937	23.853102937624477	26.4227324382006	25.24500001979798
41	23.76841707219532	23.964021128405747	26.900556719236906	25.367005080162023
42	24.43155045911519	23.872851424068802	26.776423375872593	24.919174740943415
43	23.825187783853558	23.174873589888776	26.8933799509802	26.10655867527747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	47.5
2	66.0
3	225.5
4	385.0
5	385.0
6	599.0
7	813.0
8	785.0
9	757.0
10	1059.0
11	1361.0
12	1361.0
13	2471.5
14	3582.0
15	5252.5
16	6923.0
17	7016.0
18	7109.0
19	7109.0
20	8942.5
21	10776.0
22	11247.5
23	11719.0
24	14316.5
25	16914.0
26	16914.0
27	20546.5
28	24179.0
29	29527.5
30	34876.0
31	40674.0
32	46472.0
33	46472.0
34	54178.5
35	61885.0
36	70403.0
37	78921.0
38	89164.5
39	99408.0
40	99408.0
41	105709.0
42	112010.0
43	115490.5
44	118971.0
45	123242.0
46	127513.0
47	127513.0
48	132951.5
49	138390.0
50	141820.5
51	145251.0
52	149745.0
53	154239.0
54	154239.0
55	151209.5
56	148180.0
57	143086.5
58	137993.0
59	132102.0
60	126211.0
61	126211.0
62	119240.0
63	112269.0
64	100299.0
65	88329.0
66	78307.5
67	68286.0
68	68286.0
69	58900.0
70	49514.0
71	42738.0
72	35962.0
73	28341.5
74	20721.0
75	20721.0
76	16422.5
77	12124.0
78	9867.0
79	7610.0
80	6109.5
81	4609.0
82	4609.0
83	3460.0
84	2311.0
85	1999.0
86	1687.0
87	1465.5
88	1244.0
89	1244.0
90	976.0
91	708.0
92	390.0
93	72.0
94	45.0
95	18.0
96	18.0
97	12.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2020408.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.88595274628202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.46942025312238	48.8959286927808
2	9.337012317664819	10.809637076235976
3	2.52844307483578	4.390840090548239
4	1.123254935079071	2.6008272837606077
5	0.5889451620365088	1.7045825909898382
6	0.3665776518069101	1.2731817978202693
7	0.25443748411561495	1.0309849327679559
8	0.1841934809317636	0.8529772106711414
9	0.14263730431047544	0.7431026631455908
>10	0.8050366898598288	8.992222906695678
>50	0.10479384233394909	4.25195045449692
>100	0.08685513571995501	10.243758263507912
>500	0.0065085998154231295	2.578848678585632
>1k	0.0018840683676224843	1.631157357993534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3234	0.16006667960134785	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2726	0.13492324322612065	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2063	0.10210808905923952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.9494953494541695E-5	0.0
2	0.0	0.0	0.0	3.4646467446179186E-4	0.0
3	0.0	0.0	0.0	8.909091629017505E-4	0.0
4	0.0	0.0	0.0	0.001336363744352626	0.0
5	0.0	0.0	0.0	0.0014353536513417092	0.0
6	0.0	0.0	0.0	0.001484848604836251	0.0
7	0.0	0.0	0.0	0.0015838385118253342	0.0
8	0.0	0.0	0.0	0.0016828284188144178	0.0
9	4.9494953494541695E-5	0.0	0.0	0.0028212123491888766	0.0
10	4.9494953494541695E-5	0.0	0.0	0.004058586186552419	0.0
11	4.9494953494541695E-5	0.0	0.0	0.0047515155354760025	0.0
12	4.9494953494541695E-5	0.0	0.0	0.005196970116926878	0.0
13	4.9494953494541695E-5	0.0	0.0	0.0055929297448832115	0.0
14	4.9494953494541695E-5	0.0	0.0	0.00584040451235592	0.0
15	4.9494953494541695E-5	0.0	0.0	0.00613737423332317	0.0
16	4.9494953494541695E-5	0.0	0.0	0.006978788442730379	0.0
17	4.9494953494541695E-5	0.0	0.0	0.008216162280093921	0.0
18	4.9494953494541695E-5	0.0	0.0	0.008810101722028422	0.0
19	9.898990698908339E-5	0.0	0.0	0.009602020977941089	0.0
20	9.898990698908339E-5	0.0	0.0	0.01024545537337013	0.0
21	1.4848486048362508E-4	0.0	0.0	0.012373738373635423	0.0
22	1.4848486048362508E-4	0.0	0.0	0.01514545576932976	0.0
23	1.9797981397816678E-4	0.0	0.0	0.018857577281420387	0.0
24	1.9797981397816678E-4	0.0	0.0	0.02454949693329268	0.0
25	1.9797981397816678E-4	0.0	0.0	0.02687575974753614	0.0
26	1.9797981397816678E-4	0.0	0.0	0.03137980051553944	0.0
27	1.9797981397816678E-4	0.0	0.0	0.037616164655851686	0.0
28	1.9797981397816678E-4	0.0	0.0	0.05691919651872295	0.0
29	1.9797981397816678E-4	0.0	0.0	0.08844748189474601	0.0
30	1.9797981397816678E-4	0.0	0.0	0.13135960657451365	0.0
31	1.9797981397816678E-4	0.0	0.0	0.2891495183151126	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1960	0.0	17.744898	1
GACGGAC	395	0.0	15.92405	7
CGCAAGA	455	0.0	15.857143	2
CGAATTA	105	9.350029E-6	15.857142	15
AAGACGG	430	0.0	15.488372	5
TAATTCG	75	0.004105838	14.8	5
CTTATAC	875	0.0	14.588572	37
CGGACCA	445	0.0	14.550563	9
TCTTATA	1305	0.0	14.45977	37
CAAGACG	520	0.0	14.230769	4
TCTATAC	145	8.918814E-7	14.034483	3
CGTTATT	410	0.0	13.987805	2
ACGGACC	450	0.0	13.9777775	8
TTAACGG	200	9.840733E-10	13.875001	35
GCGCAAG	540	0.0	13.703703	1
ACCGGAC	95	0.0012456451	13.631579	3
GCGTTAT	435	0.0	13.609196	1
CTAGACT	165	2.5976624E-7	13.454545	4
TAGACTG	235	5.638867E-11	13.3829775	5
CTAGCGG	360	0.0	13.361111	29
>>END_MODULE