FastQCFastQC Report
Fri 10 Feb 2017
ERR1630671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838407
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113741.3566203526449565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT94751.130119381159747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89411.0664271648495303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67430.8042633231831319No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22660.2702744609718192No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.24916299601506192No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20890.24916299601506192No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.23282248359090515No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17430.20789425660806743No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG17150.20455458983524707No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.20455458983524707No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG16000.1908381013040206No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC15700.1872598869045702No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.18296602962522976No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT15300.1824889343719697No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG15170.18093837479887454No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG14870.17736016039942415No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.1750939579464389No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT14140.16865317202742822No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14080.16793752914753812No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC14080.16793752914753812No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC14010.16710261245433305No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG13940.16626769576112793No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG13900.1657906005078679No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG12860.15338612392310655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12710.15159701672338136No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGG12710.15159701672338136No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC12530.14945008808371113No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA12250.14611042131089078No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT11960.1426514807247554No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC11900.14193583784486533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11860.1414587425916053No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA11740.1400274568318251No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.1397889092051951No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG11600.13835762344541494No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC11480.13692633768563478No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC11350.13537577811253962No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA11200.13358667091281443No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG11150.13299030184623936No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC11070.13203611133971926No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA10330.1232098491544083No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10290.12273275390114824No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA10180.1214207419546831No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGG10180.1214207419546831No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC10170.12130146814136811No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10160.12118219432805308No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG10120.12070509907479304No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG10120.12070509907479304No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG9920.11831962280849277No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA9710.11581487272887751No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9690.11557632510224747No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC9600.11450286078241237No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG9530.11366794408920727No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG9480.11307157502263221No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC9460.11283302739600219No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGA9450.11271375358268716No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA9400.1121173845161121No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG9390.11199811070279708No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT9340.11140174163622202No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG9200.10973190824981184No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG9140.10901626536992176No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC9060.10806207486340165No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGC8990.10722715817019657No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT8820.10519950334384134No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG8810.10508022953052636No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC8670.10341039614411617No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8530.10174056275770599No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT8520.10162128894439096No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG8510.10150201513107597No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT8410.10030927699792583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTAG200.00184165237.07
GCAGCGT454.006557E-628.7777771
GAAGTTA358.867385E-426.4285726
GGTAAGA400.001930753723.1252
ATACACT400.001930753723.1254
AAGACGG5150.022.631075
AGCGTCA502.7012578E-422.1999993
TTGGTAA705.0992057E-621.14285933
GTAAACA705.0992057E-621.14285936
TGAAGTT450.003824957920.5555555
GTTAGCC450.003824957920.5555559
CCTATAG450.003824957920.5555552
TATGCTA656.899284E-519.92307736
GCACCGC656.899284E-519.92307710
CGCAAGA5850.019.9230772
AGTGATT759.26087E-619.73333430
GCGCAAG6050.019.570251
CAAGACG6200.019.395164
CGTCACT1052.257184E-819.380951
GACGGAC6050.019.2644637
Produced by FastQC (version 0.11.5)