##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630671.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838407
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970412937869078	33.0	31.0	34.0	30.0	34.0
2	32.0831457752619	33.0	31.0	34.0	30.0	34.0
3	32.13944659336098	34.0	31.0	34.0	30.0	34.0
4	35.750197696345566	37.0	35.0	37.0	35.0	37.0
5	35.74767386245583	37.0	35.0	37.0	35.0	37.0
6	35.807741347579395	37.0	35.0	37.0	35.0	37.0
7	35.771910301321434	37.0	35.0	37.0	35.0	37.0
8	35.806842023027	37.0	35.0	37.0	35.0	37.0
9	37.467050012702664	39.0	37.0	39.0	35.0	39.0
10	37.39575051257921	39.0	37.0	39.0	34.0	39.0
11	37.51004822240272	39.0	37.0	39.0	35.0	39.0
12	37.44771453482616	39.0	37.0	39.0	35.0	39.0
13	37.49212971742841	39.0	37.0	39.0	35.0	39.0
14	38.646632244244145	40.0	38.0	41.0	34.0	41.0
15	38.60245560926853	40.0	38.0	41.0	34.0	41.0
16	38.5240760155867	40.0	38.0	41.0	34.0	41.0
17	38.594918697005156	40.0	38.0	41.0	34.0	41.0
18	38.59132616974811	40.0	38.0	41.0	34.0	41.0
19	38.59579297405675	40.0	38.0	41.0	34.0	41.0
20	38.59037675019412	40.0	38.0	41.0	34.0	41.0
21	38.48104560195704	40.0	38.0	41.0	34.0	41.0
22	38.3786776589413	40.0	38.0	41.0	34.0	41.0
23	38.2918797195157	40.0	38.0	41.0	34.0	41.0
24	38.18067358693332	40.0	37.0	41.0	33.0	41.0
25	38.09803591811614	40.0	37.0	41.0	33.0	41.0
26	37.79436478941612	40.0	37.0	41.0	33.0	41.0
27	37.59238055025781	40.0	36.0	41.0	33.0	41.0
28	37.37507916799359	39.0	36.0	41.0	32.0	41.0
29	37.197896725576	39.0	35.0	41.0	32.0	41.0
30	37.01965274621991	39.0	35.0	41.0	31.0	41.0
31	36.854856889315094	39.0	35.0	41.0	31.0	41.0
32	36.64992658696791	39.0	35.0	41.0	31.0	41.0
33	36.43688327983903	39.0	35.0	41.0	30.0	41.0
34	36.26767190636529	38.0	35.0	41.0	30.0	41.0
35	36.06384488679126	38.0	35.0	40.0	30.0	41.0
36	35.883587565466414	38.0	35.0	40.0	29.0	41.0
37	35.55931188551622	38.0	35.0	40.0	27.0	41.0
38	35.26437994911779	38.0	35.0	40.0	26.0	41.0
39	35.03415882739529	38.0	34.0	40.0	25.0	41.0
40	34.6922568633134	38.0	34.0	40.0	23.0	41.0
41	34.37395918688656	37.0	33.0	40.0	22.0	41.0
42	34.05441987006311	37.0	33.0	40.0	20.0	41.0
43	33.12971504293261	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	3.0
13	3.0
14	3.0
15	9.0
16	12.0
17	19.0
18	54.0
19	121.0
20	265.0
21	515.0
22	968.0
23	1518.0
24	2516.0
25	3783.0
26	5595.0
27	7618.0
28	10839.0
29	14549.0
30	18008.0
31	22438.0
32	28021.0
33	34883.0
34	45686.0
35	59203.0
36	82393.0
37	126161.0
38	171962.0
39	201253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.77262952241573	19.727173079423242	12.035801227804633	21.4643961703564
2	20.856338270076467	22.277962850978106	31.194276765341893	25.671422113603537
3	23.640427620475496	24.71675451183017	27.102230778130433	24.540587089563896
4	14.841479138413682	15.756786381793091	31.60064264730614	37.80109183248709
5	15.271222687787672	38.14149929568813	29.575015475777278	17.012262540746917
6	33.67970448720013	31.814381320766643	16.382258258817018	18.123655933216206
7	28.450621237656655	32.092170031977304	20.41096985115821	19.04623887920783
8	30.616395139830654	28.093276892964873	19.999713742848044	21.29061422435643
9	28.710995972123328	11.933464295980354	21.742423429193696	37.61311630270263
10	21.577467745379035	25.636236338675605	27.400415311417962	25.385880604527394
11	36.041803086090646	21.606689829641212	18.82200411017561	23.529502974092534
12	23.388879148194135	28.319897138263393	25.444444046865065	22.846779666677403
13	29.86270391349309	18.129858171508587	27.82312170580637	24.184316209191955
14	24.04810551438621	22.412503712397438	25.394110497646132	28.145280275570215
15	28.012409247537295	25.732609579834136	24.00361638201971	22.251364790608857
16	25.95744071793294	24.58698460294344	26.260276929939753	23.19529774918387
17	23.80323637565049	26.48975974675784	25.33673979344161	24.37026408415006
18	23.76399529106985	21.116832278356455	29.554500379887095	25.5646720506866
19	22.410953152824344	25.12574441768735	29.533866010183594	22.929436419304704
20	23.973559381064327	22.131613882040586	30.108050147482068	23.786776589413016
21	26.174638331979576	23.758747243283988	28.953718182219372	21.11289624251706
22	25.989048278461414	23.26888969199923	28.38943377142605	22.352628258113302
23	22.796207569831836	22.920610157119395	29.219340964471908	25.063841308576862
24	22.359069044032314	25.404845140844483	29.140143152430742	23.09594266269246
25	22.475718833454398	24.7797310852605	29.413160911108804	23.331389170176298
26	23.180388522519493	26.862251865740628	28.619155135870766	21.33820447586912
27	24.23942071094349	24.667375153117757	28.655414375118525	22.437789760820223
28	21.732762250315183	24.52508149383295	30.963124115137397	22.779032140714474
29	20.76079994561114	25.843414952403787	30.597311329700254	22.798473772284822
30	19.625790338105478	27.126085540793433	31.121519739219732	22.126604381881354
31	22.06160015362467	27.179281661531927	27.84268261119003	22.916435573653366
32	21.34178269026857	25.84914009544291	28.25191106467384	24.557166149614687
33	20.269749656193234	25.5297248233853	29.85435474656104	24.34617077386043
34	22.382327437628742	25.662595851418224	29.21421219049936	22.74086452045367
35	22.259356136100962	25.898519454155323	29.840518984216498	22.00160542552722
36	19.50114920319129	26.999297477239576	28.981986075975037	24.5175672435941
37	22.086647654420823	26.00515024325894	28.943818455714233	22.964383646606006
38	21.442688336333067	24.36620877449735	29.527186676637957	24.66391621253162
39	22.542512168910804	25.149479906537042	28.74844794950424	23.559559975047918
40	20.682317776449864	26.471153031880696	29.502377723468438	23.344151468201005
41	20.26092339400792	26.253120501140852	30.141446815210273	23.34450928964095
42	21.016880822798473	26.75228140986418	29.62117444153019	22.609663325807155
43	20.64081048941624	24.301800915307243	29.54841741540803	25.50897117986849
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2829.0
1	1899.5
2	970.0
3	1484.0
4	1998.0
5	1998.0
6	2634.0
7	3270.0
8	3218.5
9	3167.0
10	4880.0
11	6593.0
12	6593.0
13	11727.0
14	16861.0
15	21619.5
16	26378.0
17	24195.0
18	22012.0
19	22012.0
20	22856.5
21	23701.0
22	17530.5
23	11360.0
24	10146.5
25	8933.0
26	8933.0
27	8991.0
28	9049.0
29	9432.5
30	9816.0
31	10473.5
32	11131.0
33	11131.0
34	12595.0
35	14059.0
36	16166.5
37	18274.0
38	22681.0
39	27088.0
40	27088.0
41	33666.0
42	40244.0
43	40971.0
44	41698.0
45	44504.0
46	47310.0
47	47310.0
48	52322.5
49	57335.0
50	53813.0
51	50291.0
52	55099.5
53	59908.0
54	59908.0
55	58925.0
56	57942.0
57	57532.5
58	57123.0
59	54776.0
60	52429.0
61	52429.0
62	48551.0
63	44673.0
64	38734.5
65	32796.0
66	29319.0
67	25842.0
68	25842.0
69	21553.5
70	17265.0
71	15537.0
72	13809.0
73	10777.0
74	7745.0
75	7745.0
76	5732.5
77	3720.0
78	3140.0
79	2560.0
80	1996.0
81	1432.0
82	1432.0
83	1413.0
84	1394.0
85	1830.5
86	2267.0
87	1915.5
88	1564.0
89	1564.0
90	1491.0
91	1418.0
92	765.5
93	113.0
94	71.5
95	30.0
96	30.0
97	18.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	838407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.72986348361292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86371498697386	19.502889054384223
2	8.94995525999332	4.426623435281564
3	3.2585728546679342	2.417521855420346
4	1.7596238945838005	1.7406103478224275
5	1.084605543625105	1.3411073513709313
6	0.7938820292893367	1.1779556523851336
7	0.5769392242375285	0.9987339777614938
8	0.48716311225950776	0.9637981808343697
9	0.3737966867842701	0.8319546931321636
>10	2.884922226621855	14.444025405807986
>50	0.43448962441105493	7.653293550269991
>100	0.44950543082867067	23.047444745672575
>500	0.059578844818886305	9.908158833175541
>1k	0.02131275749618697	7.1697145475535144
>5k	0.0014531425565582027	3.013714176193719
>10k+	4.843808521860675E-4	1.362454192934034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11374	1.3566203526449565	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9475	1.130119381159747	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8941	1.0664271648495303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6743	0.8042633231831319	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2266	0.2702744609718192	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2089	0.24916299601506192	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2089	0.24916299601506192	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1952	0.23282248359090515	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1743	0.20789425660806743	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG	1715	0.20455458983524707	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1715	0.20455458983524707	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1600	0.1908381013040206	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1570	0.1872598869045702	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1534	0.18296602962522976	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1530	0.1824889343719697	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG	1517	0.18093837479887454	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1487	0.17736016039942415	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1468	0.1750939579464389	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1414	0.16865317202742822	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1408	0.16793752914753812	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1408	0.16793752914753812	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1401	0.16710261245433305	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	1394	0.16626769576112793	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	1390	0.1657906005078679	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1286	0.15338612392310655	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1271	0.15159701672338136	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGG	1271	0.15159701672338136	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1253	0.14945008808371113	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1225	0.14611042131089078	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1196	0.1426514807247554	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1190	0.14193583784486533	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1186	0.1414587425916053	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	1174	0.1400274568318251	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.1397889092051951	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	1160	0.13835762344541494	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1148	0.13692633768563478	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1135	0.13537577811253962	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	1120	0.13358667091281443	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	1115	0.13299030184623936	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1107	0.13203611133971926	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	1033	0.1232098491544083	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1029	0.12273275390114824	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	1018	0.1214207419546831	No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGG	1018	0.1214207419546831	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	1017	0.12130146814136811	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1016	0.12118219432805308	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1012	0.12070509907479304	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	1012	0.12070509907479304	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG	992	0.11831962280849277	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	971	0.11581487272887751	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	969	0.11557632510224747	No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC	960	0.11450286078241237	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	953	0.11366794408920727	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG	948	0.11307157502263221	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	946	0.11283302739600219	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGA	945	0.11271375358268716	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA	940	0.1121173845161121	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	939	0.11199811070279708	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	934	0.11140174163622202	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	920	0.10973190824981184	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	914	0.10901626536992176	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	906	0.10806207486340165	No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGC	899	0.10722715817019657	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	882	0.10519950334384134	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG	881	0.10508022953052636	No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC	867	0.10341039614411617	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	853	0.10174056275770599	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	852	0.10162128894439096	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	851	0.10150201513107597	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	841	0.10030927699792583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0011927381331501288	0.0
3	0.0	0.0	0.0	0.001908381013040206	0.0
4	0.0	0.0	0.0	0.0025047500796152706	0.0
5	0.0	0.0	0.0	0.002743297706245296	0.0
6	0.0	0.0	0.0	0.002743297706245296	0.0
7	0.0	0.0	0.0	0.0033396667728203605	0.0
8	0.0	0.0	0.0	0.003578214399450386	0.0
9	0.0	0.0	0.0	0.005844416852435631	0.0
10	0.0	0.0	0.0	0.008945535998625966	0.0
11	0.0	0.0	0.0	0.011808107518186275	0.0
12	0.0	0.0	0.0	0.01705615530404684	0.0
13	0.0	0.0	0.0	0.018845262503772036	0.0
14	0.0	0.0	0.0	0.02242347690322242	0.0
15	0.0	0.0	0.0	0.02910281044886314	0.0
16	0.0	0.0	0.0	0.04198438228688453	0.0
17	0.0	0.0	0.0	0.0620223829238067	0.0
18	0.0	0.0	0.0	0.06965590697596752	0.0
19	0.0	0.0	0.0	0.08408803838708408	0.0
20	0.0	0.0	0.0	0.09279502675908002	0.0
21	0.0	0.0	0.0	0.11331012264926223	0.0
22	0.0	0.0	0.0	0.15123919528343632	0.0
23	0.0	0.0	0.0	0.20598587559502723	0.0
24	0.0	0.0	0.0	0.29723034278101207	0.0
25	0.0	0.0	0.0	0.32454404603015	0.0
26	0.0	0.0	0.0	0.3642622258640493	0.0
27	0.0	0.0	0.0	0.41662342990934	0.0
28	0.0	0.0	0.0	0.4983259920301238	0.0
29	0.0	0.0	0.0	0.6308391986231031	0.0
30	0.0	0.0	0.0	0.8246591452599991	0.0
31	0.0	0.0	0.0	1.3016351247067355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTTAG	20	0.001841652	37.0	7
GCAGCGT	45	4.006557E-6	28.777777	1
GAAGTTA	35	8.867385E-4	26.428572	6
GGTAAGA	40	0.0019307537	23.125	2
ATACACT	40	0.0019307537	23.125	4
AAGACGG	515	0.0	22.63107	5
AGCGTCA	50	2.7012578E-4	22.199999	3
TTGGTAA	70	5.0992057E-6	21.142859	33
GTAAACA	70	5.0992057E-6	21.142859	36
TGAAGTT	45	0.0038249579	20.555555	5
GTTAGCC	45	0.0038249579	20.555555	9
CCTATAG	45	0.0038249579	20.555555	2
TATGCTA	65	6.899284E-5	19.923077	36
GCACCGC	65	6.899284E-5	19.923077	10
CGCAAGA	585	0.0	19.923077	2
AGTGATT	75	9.26087E-6	19.733334	30
GCGCAAG	605	0.0	19.57025	1
CAAGACG	620	0.0	19.39516	4
CGTCACT	105	2.257184E-8	19.38095	1
GACGGAC	605	0.0	19.264463	7
>>END_MODULE