FastQCFastQC Report
Fri 10 Feb 2017
ERR1630670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479102
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94501.9724401066996171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90031.879140558795413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69321.4468735258880154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46160.9634691568809982No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT15230.3178863791009013No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.27071479559676226No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12880.2688362812094293No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.25213837554424734No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.2519296517234326No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC11620.24253707978676772No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11170.2331445078501029TruSeq Adapter, Index 11 (95% over 21bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT10800.22542172647995626No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10350.2160291545432914No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9590.20016614416136858No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA9480.19787018213240604No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC9360.19536549628262875No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC9180.19160846750796282No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG8810.18388568613781617No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA8410.17553673330522518No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8350.17428439038033655No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC8220.17157098070974447No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.1642656469812274No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT7840.16363947551878308No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7840.16363947551878308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7720.16113478966900577No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG7160.1494462557033784No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7100.14819391277848976No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7080.1477764651368602No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA6970.14548050310789767No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC6780.14151475051241696No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC6770.14130602669160222No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC6530.13629665499204763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6290.13128728329249303No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC6260.13066111183004872No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG6170.12878259744271575No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC6090.12711280687619755No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT6070.126695359234568No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5970.12460812102642026No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC5810.12126853989338386No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG5790.12085109225175432No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.11918130168523612No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT5360.11187596795671903No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC5290.1104149012110156No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG5280.11020617739020083No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5210.1087451106444974No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC5200.10853638682368263No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG5000.10436191040738714No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG4970.10373573894494283No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4910.10248339602005418No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.10123105309516554No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT4850.10123105309516554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG250.00549318429.626
GATACGG250.00549318429.627
CGGGGTG250.00549318429.631
AGCGGGG250.00549318429.629
CTTGCAG250.00549318429.63
GTGGGCA405.9330097E-527.75000216
GTCCCCA358.861816E-426.4285711
AGAATAT358.861816E-426.428576
CCGTCTT1550.026.25806437
GCCGTCT1550.026.25806436
TAAGAAT451.3216393E-424.6666664
ACTGAGG551.899658E-523.5454548
AATACTG952.8012437E-1023.3684225
CCATACC400.001929552223.1250023
CATACCT400.001929552223.1250024
ATGGGTA652.677425E-622.769235
CTGGGTA502.698916E-422.229
CTCTAAT1059.786163E-1021.1428571
AGCGAGC806.9427006E-720.812519
TGTGGGC450.003822601220.55555515
Produced by FastQC (version 0.11.5)