##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	479102
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.688133215891398	31.0	31.0	34.0	30.0	34.0
2	31.800445416633618	31.0	31.0	34.0	30.0	34.0
3	31.81965844433962	33.0	31.0	34.0	30.0	34.0
4	35.510868249349826	37.0	35.0	37.0	33.0	37.0
5	35.52516583107564	37.0	35.0	37.0	33.0	37.0
6	35.60090961841111	37.0	35.0	37.0	33.0	37.0
7	35.562617146244435	37.0	35.0	37.0	33.0	37.0
8	35.60735083552145	37.0	35.0	37.0	33.0	37.0
9	37.18157928791781	39.0	37.0	39.0	34.0	39.0
10	37.07749080571569	39.0	37.0	39.0	33.0	39.0
11	37.24868190907156	39.0	37.0	39.0	34.0	39.0
12	37.194624526718734	39.0	37.0	39.0	34.0	39.0
13	37.26443012135203	39.0	37.0	39.0	34.0	39.0
14	38.31277264549094	40.0	38.0	41.0	34.0	41.0
15	38.25531097762063	40.0	38.0	41.0	33.0	41.0
16	38.17209279026178	40.0	37.0	41.0	33.0	41.0
17	38.27410864492321	40.0	38.0	41.0	34.0	41.0
18	38.293958280282695	40.0	38.0	41.0	34.0	41.0
19	38.372939374079	40.0	38.0	41.0	34.0	41.0
20	38.369541350276144	40.0	38.0	41.0	34.0	41.0
21	38.272816644472364	40.0	38.0	41.0	34.0	41.0
22	38.19091132994644	40.0	38.0	41.0	34.0	41.0
23	38.09299481112581	40.0	37.0	41.0	33.0	41.0
24	38.008616119323236	40.0	37.0	41.0	33.0	41.0
25	37.92398278445926	40.0	37.0	41.0	33.0	41.0
26	37.609400503441854	40.0	37.0	41.0	33.0	41.0
27	37.38744359238743	39.0	36.0	41.0	32.0	41.0
28	37.1983794682552	39.0	35.0	41.0	32.0	41.0
29	37.00730324649031	39.0	35.0	41.0	31.0	41.0
30	36.76587449019207	39.0	35.0	40.0	31.0	41.0
31	36.58889964976143	39.0	35.0	40.0	31.0	41.0
32	36.330274137866255	38.0	35.0	40.0	30.0	41.0
33	36.146194338575086	38.0	35.0	40.0	30.0	41.0
34	35.944947005021895	38.0	35.0	40.0	30.0	41.0
35	35.71836686133641	38.0	35.0	40.0	29.0	41.0
36	35.51024833960201	38.0	35.0	40.0	27.0	41.0
37	35.11797279076272	38.0	34.0	40.0	25.0	41.0
38	34.79629181259941	38.0	34.0	40.0	24.0	41.0
39	34.51839691756661	38.0	34.0	40.0	23.0	41.0
40	34.16984692194981	37.0	33.0	40.0	21.0	41.0
41	33.82895500331871	37.0	33.0	40.0	18.0	41.0
42	33.423646739107745	37.0	33.0	40.0	15.0	41.0
43	32.49651848666881	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	8.0
12	4.0
13	2.0
14	2.0
15	3.0
16	8.0
17	9.0
18	40.0
19	90.0
20	196.0
21	349.0
22	622.0
23	1023.0
24	1662.0
25	2619.0
26	3649.0
27	5398.0
28	7199.0
29	9670.0
30	12010.0
31	14437.0
32	17898.0
33	21958.0
34	27570.0
35	35164.0
36	48674.0
37	76656.0
38	104628.0
39	87548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79671969643208	20.275014506305546	13.247074735651282	20.6811910616111
2	20.209266502748893	22.155616131846664	32.492872081519174	25.142245283885266
3	23.15185492859558	26.138901528275817	29.05289479067088	21.656348752457724
4	14.074247237540233	16.589786725999893	31.578244298708835	37.75772173775104
5	12.855508847802765	41.08060496512225	30.76797842630588	15.295907760769106
6	32.02428710379001	31.89717429691381	17.285880668417164	18.79265793087902
7	26.71518799754541	33.74250159673723	20.747147788988567	18.795162616728796
8	31.76212998484665	29.014489607640964	19.09113299464415	20.13224741286824
9	28.98944274914319	11.789973742543342	22.242236517484795	36.978346990828676
10	21.38438161393607	28.623758615075705	28.416704584827446	21.575155186160778
11	32.21005130431516	23.46264469778878	19.33847072230965	24.98883327558641
12	22.689740389311673	30.391231929735213	25.86192501805461	21.057102662898505
13	30.610809389232358	19.44408497564193	28.63294246319155	21.31216317193416
14	23.118459117265218	22.933947259664954	28.15141660857187	25.796177014497957
15	27.802847827811195	25.986741862901848	26.23658427641713	19.973826032869827
16	23.721253511778283	24.823315285680295	28.691385133019693	22.76404606952173
17	22.535284761908738	27.459914590212524	27.556553719249766	22.448246928628976
18	21.02850749944688	22.106148586313562	32.3279802630755	24.53736365116405
19	21.252885606822762	25.573259973867778	32.57970119097812	20.594153228331336
20	22.51316003690237	22.35432120926233	34.35155770587474	20.78096104796056
21	23.244319581216526	24.48560014360199	32.52063234968754	19.749447925493946
22	24.5027154969088	24.736903623862975	30.606217465174428	20.15416341405379
23	21.78116559730496	23.770303609669757	32.13574562410509	22.312785168920186
24	20.29922646952006	26.384986913016434	31.6978430480357	21.617943569427805
25	20.43573184833292	25.585783403116665	32.54296579851472	21.435518950035693
26	21.50878101114168	26.674486852486528	31.470751530989226	20.345980605382568
27	21.669498353169054	26.570124942079136	31.532742505771218	20.227634198980592
28	20.19215114944208	26.415669314676204	32.45551051759333	20.93666901828838
29	19.63590216697071	26.63608166945661	33.271829380799915	20.45618678277277
30	17.8867965485429	28.146198513051502	33.94538115056919	20.02162378783641
31	20.799537468013074	28.06792708024596	30.910536795922372	20.22199865581859
32	18.810816903289904	27.842714077586816	31.61831927230527	21.728149746818005
33	18.277944988749788	27.51230426923703	32.16037503496124	22.049375707051944
34	20.165643224198604	27.27122825619597	32.32965005364202	20.233478465963405
35	18.856109972406713	29.03828412321385	32.172272292747685	19.933333611631763
36	19.57996418299235	28.612070081110076	30.813062771601874	20.994902964295704
37	19.6217089471553	27.48955337276822	31.60955287183105	21.27918480824543
38	19.171700389478648	26.450108745110647	31.384548592992722	22.993642272417983
39	19.880109037323994	27.58765356855116	31.515627152464404	21.01661024166044
40	18.43156572086946	27.799716970498977	31.437564443479676	22.33115286515189
41	18.97967447432906	27.415247692558157	31.2401117089889	22.36496612412388
42	18.452855550592567	28.365358524907013	32.01969517973208	21.16209074476834
43	17.754674369967148	26.574925589957882	31.287492016313855	24.382908023761118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	171.0
2	143.0
3	563.0
4	983.0
5	983.0
6	1535.5
7	2088.0
8	2206.0
9	2324.0
10	3366.5
11	4409.0
12	4409.0
13	8033.0
14	11657.0
15	16391.0
16	21125.0
17	18878.0
18	16631.0
19	16631.0
20	17802.5
21	18974.0
22	13693.0
23	8412.0
24	7601.0
25	6790.0
26	6790.0
27	7031.5
28	7273.0
29	7682.5
30	8092.0
31	8898.5
32	9705.0
33	9705.0
34	11254.0
35	12803.0
36	13913.5
37	15024.0
38	17612.0
39	20200.0
40	20200.0
41	21451.0
42	22702.0
43	24208.5
44	25715.0
45	26635.0
46	27555.0
47	27555.0
48	30153.0
49	32751.0
50	32877.5
51	33004.0
52	31376.0
53	29748.0
54	29748.0
55	28462.0
56	27176.0
57	25147.0
58	23118.0
59	21788.5
60	20459.0
61	20459.0
62	19363.5
63	18268.0
64	17085.5
65	15903.0
66	14979.5
67	14056.0
68	14056.0
69	11088.0
70	8120.0
71	7126.0
72	6132.0
73	4579.5
74	3027.0
75	3027.0
76	2368.0
77	1709.0
78	1279.5
79	850.0
80	713.5
81	577.0
82	577.0
83	523.0
84	469.0
85	453.0
86	437.0
87	357.5
88	278.0
89	278.0
90	222.0
91	166.0
92	105.5
93	45.0
94	23.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	479102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.77821284377071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26083465734905	24.869894610723854
2	9.37657143537959	5.959413656366267
3	3.470670032570469	3.30875073016563
4	1.8316318970363246	2.3282395310183936
5	1.2404742944978202	1.9710028078889021
6	0.8254754630706278	1.573928097766116
7	0.6247961287824754	1.3898433055089432
8	0.4913334348697205	1.2490958776440746
9	0.4019891547588788	1.1497047228733621
>10	2.8401160429547714	17.777748190561184
>50	0.32795166700096756	7.341754251296435
>100	0.27780211631269086	17.644786165588652
>500	0.021775462798857004	4.855639821861655
>1k	0.006598625090562728	3.2571537982802337
>5k	0.0019795875271688187	5.323044432456301
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9450	1.9724401066996171	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9003	1.879140558795413	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6932	1.4468735258880154	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4616	0.9634691568809982	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	1523	0.3178863791009013	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1297	0.27071479559676226	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1288	0.2688362812094293	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1208	0.25213837554424734	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.2519296517234326	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1162	0.24253707978676772	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	1117	0.2331445078501029	TruSeq Adapter, Index 11 (95% over 21bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1080	0.22542172647995626	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1035	0.2160291545432914	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	959	0.20016614416136858	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	948	0.19787018213240604	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	936	0.19536549628262875	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	918	0.19160846750796282	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	881	0.18388568613781617	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	841	0.17553673330522518	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	835	0.17428439038033655	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	822	0.17157098070974447	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	787	0.1642656469812274	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	784	0.16363947551878308	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	784	0.16363947551878308	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	772	0.16113478966900577	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	716	0.1494462557033784	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	710	0.14819391277848976	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	708	0.1477764651368602	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	697	0.14548050310789767	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	678	0.14151475051241696	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	677	0.14130602669160222	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	653	0.13629665499204763	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	629	0.13128728329249303	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	626	0.13066111183004872	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	617	0.12878259744271575	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	609	0.12711280687619755	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	607	0.126695359234568	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	597	0.12460812102642026	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	581	0.12126853989338386	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	579	0.12085109225175432	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.11918130168523612	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	536	0.11187596795671903	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	529	0.1104149012110156	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	528	0.11020617739020083	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	521	0.1087451106444974	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	520	0.10853638682368263	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG	500	0.10436191040738714	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	497	0.10373573894494283	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	491	0.10248339602005418	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.10123105309516554	No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT	485	0.10123105309516554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.1744764162954864E-4	0.0
3	0.0	0.0	0.0	0.0018785143873329688	0.0
4	0.0	0.0	0.0	0.002087238208147743	0.0
5	0.0	0.0	0.0	0.002087238208147743	0.0
6	0.0	0.0	0.0	0.002087238208147743	0.0
7	0.0	0.0	0.0	0.002295962028962517	0.0
8	0.0	0.0	0.0	0.0025046858497772915	0.0
9	0.0	0.0	0.0	0.0043832002371102605	0.0
10	0.0	0.0	0.0	0.007514057549331875	0.0
11	0.0	0.0	0.0	0.008975124295035296	0.0
12	0.0	0.0	0.0	0.010853638682368263	0.0
13	0.0	0.0	0.0	0.012314705428071684	0.0
14	0.0	0.0	0.0	0.013358324532145556	0.0
15	0.0	0.0	0.0	0.017324077127626267	0.0
16	0.0	0.0	0.0	0.028177715809994532	0.0
17	0.0	0.0	0.0	0.04404072619191738	0.0
18	0.0	0.0	0.0	0.05030244081636061	0.0
19	0.0	0.0	0.0	0.06157352714035842	0.0
20	0.0	0.0	0.0	0.0699224799729494	0.0
21	0.0	0.0	0.0	0.08641166181731656	0.0
22	0.0	0.0	0.0	0.12043364461012478	0.0
23	0.0	0.0	0.0	0.17344949509707744	0.0
24	0.0	0.0	0.0	0.2544343375732099	0.0
25	0.0	0.0	0.0	0.27488927201305774	0.0
26	0.0	0.0	0.0	0.31350317886379103	0.0
27	0.0	0.0	0.0	0.3446030281651924	0.0
28	0.0	0.0	0.0	0.4022108027100701	0.0
29	0.0	0.0	0.0	0.4884137407065719	0.0
30	0.0	0.0	0.0	0.6493398065547629	0.0
31	0.0	0.0	0.0	1.0897470684739368	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	0.005493184	29.6	26
GATACGG	25	0.005493184	29.6	27
CGGGGTG	25	0.005493184	29.6	31
AGCGGGG	25	0.005493184	29.6	29
CTTGCAG	25	0.005493184	29.6	3
GTGGGCA	40	5.9330097E-5	27.750002	16
GTCCCCA	35	8.861816E-4	26.42857	11
AGAATAT	35	8.861816E-4	26.42857	6
CCGTCTT	155	0.0	26.258064	37
GCCGTCT	155	0.0	26.258064	36
TAAGAAT	45	1.3216393E-4	24.666666	4
ACTGAGG	55	1.899658E-5	23.545454	8
AATACTG	95	2.8012437E-10	23.368422	5
CCATACC	40	0.0019295522	23.125002	3
CATACCT	40	0.0019295522	23.125002	4
ATGGGTA	65	2.677425E-6	22.76923	5
CTGGGTA	50	2.698916E-4	22.2	29
CTCTAAT	105	9.786163E-10	21.142857	1
AGCGAGC	80	6.9427006E-7	20.8125	19
TGTGGGC	45	0.0038226012	20.555555	15
>>END_MODULE