FastQCFastQC Report
Fri 10 Feb 2017
ERR1630669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968082
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93110.3137042709736456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81820.2756662383316903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72760.2451414752018307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.17769050855063978No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG39410.13277935043573594No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT38000.1280288078294333No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG35030.11802234574381705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.017.6817681
TTATACG700.002593789515.8571434
CGGCCTT9350.015.828876524
CTTATAC21300.015.72065737
ATCTCGC10150.015.12807911
TGCGTTA1353.9780025E-715.07407537
TATACTG3550.014.5915495
GGACCGT2059.276846E-1114.4390246
TCTCGCC11150.014.1031412
CTAGCAC8000.013.643753
CGAACTA1652.599063E-713.45454529
GTATTAG6050.013.4545451
TCTTATA32350.013.32457537
GACGGTA2651.8189894E-1213.2641528
CTAGTAC1551.8901865E-613.1290323
TCTAGCG2408.185452E-1113.10416728
TAGAACA15700.013.07961754
TACCGTC2551.6370905E-1113.0588247
TTATGCG850.00940931913.0588234
TTGTACG850.00940931913.0588234
Produced by FastQC (version 0.11.5)