##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2968082
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14838201909516	33.0	31.0	34.0	30.0	34.0
2	32.32366962907359	34.0	31.0	34.0	30.0	34.0
3	32.430126256619594	34.0	31.0	34.0	30.0	34.0
4	35.98831366518849	37.0	35.0	37.0	35.0	37.0
5	35.96272272801088	37.0	35.0	37.0	35.0	37.0
6	36.001030294985114	37.0	35.0	37.0	35.0	37.0
7	35.980946954969575	37.0	35.0	37.0	35.0	37.0
8	35.98204901347065	37.0	35.0	37.0	35.0	37.0
9	37.73360439502682	39.0	37.0	39.0	35.0	39.0
10	37.656644594050974	39.0	37.0	39.0	35.0	39.0
11	37.74713973535771	39.0	37.0	39.0	35.0	39.0
12	37.69021576897134	39.0	37.0	39.0	35.0	39.0
13	37.7275708689989	39.0	37.0	39.0	35.0	39.0
14	39.0542077341529	40.0	38.0	41.0	36.0	41.0
15	39.038149552471936	40.0	38.0	41.0	36.0	41.0
16	39.020669240270315	40.0	38.0	41.0	36.0	41.0
17	39.01595946473177	40.0	38.0	41.0	36.0	41.0
18	38.99961389206902	40.0	38.0	41.0	36.0	41.0
19	39.038674470584034	40.0	39.0	41.0	36.0	41.0
20	39.01875925260825	40.0	39.0	41.0	36.0	41.0
21	38.949691416881336	40.0	38.0	41.0	35.0	41.0
22	38.914476756369936	40.0	38.0	41.0	35.0	41.0
23	38.89639841486859	40.0	38.0	41.0	35.0	41.0
24	38.859197960164174	40.0	38.0	41.0	35.0	41.0
25	38.8149791683653	40.0	38.0	41.0	35.0	41.0
26	38.65934094812744	40.0	38.0	41.0	35.0	41.0
27	38.56326004470227	40.0	38.0	41.0	34.0	41.0
28	38.49176235696992	40.0	38.0	41.0	34.0	41.0
29	38.4092818190333	40.0	38.0	41.0	34.0	41.0
30	38.34631994668611	40.0	38.0	41.0	34.0	41.0
31	38.300168593724834	40.0	38.0	41.0	34.0	41.0
32	38.201160884369095	40.0	38.0	41.0	34.0	41.0
33	38.18499084594024	40.0	38.0	41.0	34.0	41.0
34	38.12081000457535	40.0	38.0	41.0	34.0	41.0
35	38.081621397252505	40.0	38.0	41.0	33.0	41.0
36	38.03611220983787	40.0	38.0	41.0	33.0	41.0
37	37.87568402759762	40.0	37.0	41.0	33.0	41.0
38	37.77578887645287	40.0	37.0	41.0	33.0	41.0
39	37.726160530605284	40.0	37.0	41.0	33.0	41.0
40	37.643511533711	40.0	37.0	41.0	33.0	41.0
41	37.53327637174445	40.0	37.0	41.0	33.0	41.0
42	37.4634632735888	40.0	37.0	41.0	32.0	41.0
43	36.66230885804368	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	5.0
11	5.0
12	2.0
13	8.0
14	2.0
15	8.0
16	9.0
17	18.0
18	54.0
19	122.0
20	246.0
21	473.0
22	933.0
23	1657.0
24	2784.0
25	4480.0
26	7161.0
27	10978.0
28	16378.0
29	23529.0
30	33131.0
31	44874.0
32	60130.0
33	80500.0
34	111117.0
35	154207.0
36	222341.0
37	349132.0
38	652910.0
39	1190881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11380211193626	19.614990421423666	12.433719823104617	24.837487643535454
2	17.966990130326586	21.27606312763596	36.292865224074	24.46408151796345
3	20.168681323494432	23.97278781381377	29.98967009671566	25.868860765976144
4	14.494714094826222	15.673320346270755	34.725758924450204	35.10620663445282
5	13.801438100429838	37.684032988306924	34.71174313917203	13.802785772091203
6	34.310945587082834	34.84273682465646	16.04679385542583	14.799523732834874
7	27.928035680954906	31.04233643140587	22.205350121728443	18.82427776591078
8	25.893051472297596	34.71868364822805	20.625272482364032	18.762992397110324
9	26.01939569055033	14.542421671638452	20.518941188282533	38.919241449528684
10	16.58609836251155	27.558807337533125	33.72086755015529	22.13422674980004
11	34.33011621646572	22.310367435940112	22.49226941843251	20.867246929161663
12	20.858857673069682	26.296510675917983	29.673438941376958	23.17119270963538
13	28.89515855694014	20.717149997877417	26.376966674101322	24.010724771081122
14	22.22381322348911	21.81550240188782	25.970980586115882	29.98970378850719
15	24.956958736315237	28.509353852083603	23.574988831171105	22.958698580430056
16	24.555689499144563	27.342202809760646	24.74278001753321	23.35932767356158
17	23.052429144477816	27.314036472038172	26.235225307117528	23.398309076366488
18	22.186954403550846	26.147289731213625	28.058995674647804	23.606760190587725
19	23.459594445166946	26.442497208635075	27.89811737007266	22.19979097612532
20	23.70874524356133	25.56806045116004	28.176411568144005	22.54678273713462
21	23.45790985559024	26.604723184871578	26.890362193497346	23.04700476604083
22	23.601066277818468	26.093045946843784	27.003297078719523	23.302590696618218
23	22.94249282870217	26.5996020325584	27.25635612493186	23.201549013807572
24	23.933671643842725	26.62507302695815	26.84740515929142	22.593850169907707
25	23.650559519582007	25.882169023632095	27.252178342781637	23.21509311400426
26	23.43631341721691	26.48949725782509	27.05420537572749	23.019983949230514
27	23.593384549348702	26.079063853357155	26.742657379412027	23.58489421788212
28	22.752909117740007	26.38815234889063	27.519994393685888	23.338944139683473
29	22.87982609644882	26.87634640821918	27.381858048396236	22.861969446935763
30	22.520098838239644	27.343685248588145	27.286847196270188	22.849368716902028
31	23.17540418357714	26.662571990935565	27.22377616251842	22.93824766296888
32	22.393249243113903	26.665031491717546	27.331859429759692	23.609859835408862
33	22.569962689710053	26.344689937811694	27.586737832714864	23.49860953976339
34	22.995523708576783	26.246141447574562	27.405408610678546	23.352926233170106
35	23.09221915027954	26.28909848178049	27.467199356352012	23.151483011587956
36	22.73832057200576	26.522009836655457	27.36130605556046	23.378363535778323
37	23.308520451928214	25.368470278112266	27.467603657850425	23.8554056121091
38	22.640210075058572	25.67250500491563	28.32731710242507	23.359967817600726
39	22.556081671598022	25.605525723345917	28.414410383540616	23.423982221515445
40	22.298036240238645	25.150989763759895	29.034406731350415	23.516567264651044
41	21.644786094184727	25.74457174700699	29.20839114283231	23.402251015975974
42	22.24712794323068	25.114838471443846	29.012810292977083	23.62522329234839
43	21.49580099202111	25.202571896598545	28.987440373951934	24.314186737428415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	669.0
1	661.5
2	654.0
3	1508.0
4	2362.0
5	2362.0
6	3076.0
7	3790.0
8	3661.0
9	3532.0
10	5454.0
11	7376.0
12	7376.0
13	11518.0
14	15660.0
15	21019.5
16	26379.0
17	26960.0
18	27541.0
19	27541.0
20	31786.0
21	36031.0
22	37786.5
23	39542.0
24	43333.0
25	47124.0
26	47124.0
27	53190.5
28	59257.0
29	74234.5
30	89212.0
31	95364.5
32	101517.0
33	101517.0
34	111633.0
35	121749.0
36	130042.0
37	138335.0
38	157313.5
39	176292.0
40	176292.0
41	185091.0
42	193890.0
43	187419.0
44	180948.0
45	187609.5
46	194271.0
47	194271.0
48	201108.0
49	207945.0
50	212256.5
51	216568.0
52	221333.5
53	226099.0
54	226099.0
55	201612.5
56	177126.0
57	166463.5
58	155801.0
59	142420.0
60	129039.0
61	129039.0
62	120235.5
63	111432.0
64	98445.5
65	85459.0
66	74554.5
67	63650.0
68	63650.0
69	54865.0
70	46080.0
71	39882.5
72	33685.0
73	27056.0
74	20427.0
75	20427.0
76	16178.5
77	11930.0
78	9662.0
79	7394.0
80	5753.5
81	4113.0
82	4113.0
83	3139.5
84	2166.0
85	1778.5
86	1391.0
87	1204.5
88	1018.0
89	1018.0
90	778.0
91	538.0
92	300.0
93	62.0
94	41.0
95	20.0
96	20.0
97	13.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2968082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.16120553187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.50879527444329	44.92589434835404
2	9.475578557335961	10.074663584398348
3	2.4745024764297177	3.9464260421580453
4	1.0227862760368376	2.1749020574228095
5	0.5768587124778151	1.5333252288441515
6	0.3529240440381728	1.1257120585351226
7	0.24260183037677538	0.9027904036947334
8	0.17575542535416502	0.7474696232475203
9	0.13293149022268386	0.6360118446047278
>10	0.8146712991585728	8.361029422654566
>50	0.09917130482106497	3.733917506797953
>100	0.1050487417728017	12.021078358720363
>500	0.013478921511417472	4.793047949741286
>1k	0.004641326746841447	4.0082842406261445
>5k	2.543192737995313E-4	1.0154473302003664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9311	0.3137042709736456	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8182	0.2756662383316903	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7276	0.2451414752018307	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5274	0.17769050855063978	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3941	0.13277935043573594	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3800	0.1280288078294333	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3503	0.11802234574381705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.390686645449823E-4	0.0
3	0.0	0.0	0.0	0.0011792127036921486	0.0
4	0.0	0.0	0.0	0.0021899664497139902	0.0
5	0.0	0.0	0.0	0.00242580899045242	0.0
6	0.0	0.0	0.0	0.002493192573520543	0.0
7	0.0	0.0	0.0	0.0029985694465314637	0.0
8	0.0	0.0	0.0	0.003301795570338016	0.0
9	0.0	0.0	0.0	0.005289611270847638	0.0
10	0.0	0.0	0.0	0.008086029968174733	0.0
11	0.0	0.0	0.0	0.009433701629537189	0.0
12	0.0	0.0	0.0	0.010612914333229338	0.0
13	0.0	0.0	0.0	0.011488900913114934	0.0
14	0.0	0.0	0.0	0.012398579284534592	0.0
15	0.0	0.0	0.0	0.01431901140197609	0.0
16	0.0	0.0	0.0	0.016070984561747284	0.0
17	0.0	0.0	0.0	0.018766327884472196	0.0
18	0.0	0.0	0.0	0.020450917461175264	0.0
19	0.0	3.369179153406139E-5	0.0	0.022438733161684887	0.0
20	0.0	3.369179153406139E-5	0.0	0.02472977498600106	0.0
21	0.0	3.369179153406139E-5	0.0	0.028267413097077507	0.0
22	0.0	3.369179153406139E-5	0.0	0.03406240124093607	0.0
23	0.0	3.369179153406139E-5	0.0	0.04312549316359858	0.0
24	0.0	3.369179153406139E-5	0.0	0.05902801876767556	0.0
25	0.0	3.369179153406139E-5	0.0	0.06411547928931882	0.0
26	0.0	3.369179153406139E-5	0.0	0.07274057792203854	0.0
27	0.0	3.369179153406139E-5	0.0	0.09029400131128452	0.0
28	0.0	1.0107537460218417E-4	0.0	0.12930909590772763	0.0
29	0.0	1.6845895767030695E-4	0.0	0.18483316835586078	0.0
30	0.0	1.6845895767030695E-4	0.0	0.2667716053666981	0.0
31	0.0	1.6845895767030695E-4	0.0	0.5348908823947587	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4635	0.0	17.681768	1
TTATACG	70	0.0025937895	15.857143	4
CGGCCTT	935	0.0	15.8288765	24
CTTATAC	2130	0.0	15.720657	37
ATCTCGC	1015	0.0	15.128079	11
TGCGTTA	135	3.9780025E-7	15.074075	37
TATACTG	355	0.0	14.591549	5
GGACCGT	205	9.276846E-11	14.439024	6
TCTCGCC	1115	0.0	14.10314	12
CTAGCAC	800	0.0	13.64375	3
CGAACTA	165	2.599063E-7	13.454545	29
GTATTAG	605	0.0	13.454545	1
TCTTATA	3235	0.0	13.324575	37
GACGGTA	265	1.8189894E-12	13.264152	8
CTAGTAC	155	1.8901865E-6	13.129032	3
TCTAGCG	240	8.185452E-11	13.104167	28
TAGAACA	1570	0.0	13.0796175	4
TACCGTC	255	1.6370905E-11	13.058824	7
TTATGCG	85	0.009409319	13.058823	4
TTGTACG	85	0.009409319	13.058823	4
>>END_MODULE