FastQCFastQC Report
Fri 10 Feb 2017
ERR1630668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876886
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40510.21583623086324902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.1884504439800819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29140.15525716532597078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA450.003826697420.55555515
GTACGAC450.003826697420.5555553
GGTATCA18900.018.0105821
TTAGTAC1054.801259E-717.6190473
CTTATAC7800.016.83974537
ACACCGC904.4483702E-516.4444456
TATACTG2250.015.6222225
TGTACTA1455.350921E-815.3103455
TCTTATA12200.015.01229437
CGAACGA2600.014.94230716
TAGACTA1001.0939494E-414.85
TCTAATA2650.014.66037752
ACGAACG2650.014.660377515
TCTAGCG2800.014.53571428
GTAGCAC1406.000173E-714.5357143
CGTACAC908.277456E-414.3888883
CGCAATA2850.014.28070236
TAGACTC1304.4472818E-614.2307695
GTACTAT1702.4390829E-814.1470591
CTCTTAT19300.013.99481937
Produced by FastQC (version 0.11.5)