FastQCFastQC Report
Fri 10 Feb 2017
ERR1630667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326021
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78080.3356805463063317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67160.288733420721481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57900.2489229460954996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.1732572491821871No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG37630.16177841902545162No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT34660.14900983267133014No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG32170.13830485623302627No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC27810.11956039949768296No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA27050.11629301713097172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG701.2198786E-418.532
AAGACGG4150.017.8313265
GGTATCA34150.017.3352851
GCGCAAG4700.015.7446811
CGCAAGA4300.015.4883722
CAAGACG4550.015.4505494
CGGACCA4450.015.3820229
ACGGACC4600.015.2826098
CGTCAGA2800.015.1964281
GACGGAC4550.015.0439567
TCGCCAT7150.015.00699313
CGAATTA1152.2116576E-514.47826115
TCTTATA19200.014.35677137
CGGCCTT9150.014.35519224
ATCTCGT2850.014.28070237
CTTATAC12700.014.12992137
GGGATAT6400.013.8751
TCCTCGG10300.013.83009720
GTCGCCA7600.013.63157912
AGGACCG4800.013.4895845
Produced by FastQC (version 0.11.5)