FastQCFastQC Report
Fri 10 Feb 2017
ERR1630666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362734
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140831.0334371931719617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114790.8423507448995915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105710.7757199864390263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66170.4855679831867407No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.12834493011842368No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.12467583548953795No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.11227429564390409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50250.024.0039791
GTCTATG1054.798876E-717.6190471
TATACTG1107.810213E-716.8181825
GTATCAA76800.015.7057292
GTTAGTG1201.9360432E-615.4166669
CTATGCT1252.9595358E-614.8000014
TCTTGCG750.00410492514.82
GCTATAC750.00410492514.83
TCTATGG5350.014.5233642
TCTTATA16100.014.47826137
AGTCGGT3150.014.09523911
AGTTACG800.00629941413.87520
TGCACCG800.00629941413.8755
GCAGTCG3100.013.7258069
TACGGGC3000.013.5666674
GTGTAGA1501.3058889E-613.5666671
ACACGCT5050.013.5544559
TCGGTGA3300.013.45454613
CAGTCGG3050.013.34426210
CTCTATG5600.013.2142851
Produced by FastQC (version 0.11.5)