##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630666.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1362734
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970182735588896	33.0	31.0	34.0	30.0	34.0
2	32.106268721555345	33.0	31.0	34.0	30.0	34.0
3	32.18953662270113	34.0	31.0	34.0	30.0	34.0
4	35.79146920822406	37.0	35.0	37.0	35.0	37.0
5	35.78761005449339	37.0	35.0	37.0	35.0	37.0
6	35.8539003209724	37.0	35.0	37.0	35.0	37.0
7	35.832740652247615	37.0	35.0	37.0	35.0	37.0
8	35.84097850350839	37.0	35.0	37.0	35.0	37.0
9	37.53771682514709	39.0	37.0	39.0	35.0	39.0
10	37.447247225063734	39.0	37.0	39.0	34.0	39.0
11	37.58513400267404	39.0	37.0	39.0	35.0	39.0
12	37.516528537484206	39.0	37.0	39.0	35.0	39.0
13	37.56960859566137	39.0	37.0	39.0	35.0	39.0
14	38.774774093843696	40.0	38.0	41.0	35.0	41.0
15	38.742787660688	40.0	38.0	41.0	35.0	41.0
16	38.70013590326506	40.0	38.0	41.0	35.0	41.0
17	38.73843611445814	40.0	38.0	41.0	35.0	41.0
18	38.770256704536614	40.0	38.0	41.0	35.0	41.0
19	38.821216026018284	40.0	38.0	41.0	35.0	41.0
20	38.81802684896686	40.0	38.0	41.0	35.0	41.0
21	38.74644941712763	40.0	38.0	41.0	35.0	41.0
22	38.659744308133504	40.0	38.0	41.0	34.0	41.0
23	38.634963976828935	40.0	38.0	41.0	34.0	41.0
24	38.57639128399233	40.0	38.0	41.0	34.0	41.0
25	38.52517219061093	40.0	38.0	41.0	34.0	41.0
26	38.32397445136028	40.0	38.0	41.0	34.0	41.0
27	38.17500480651396	40.0	37.0	41.0	34.0	41.0
28	38.09613541600929	40.0	37.0	41.0	33.0	41.0
29	37.97849103346655	40.0	37.0	41.0	33.0	41.0
30	37.863954374074474	40.0	37.0	41.0	33.0	41.0
31	37.768524891871785	40.0	37.0	41.0	33.0	41.0
32	37.60989231941083	40.0	37.0	41.0	33.0	41.0
33	37.53156522109231	40.0	36.0	41.0	33.0	41.0
34	37.41518153946405	40.0	36.0	41.0	32.0	41.0
35	37.334590609759495	40.0	36.0	41.0	32.0	41.0
36	37.249537327167296	40.0	36.0	41.0	32.0	41.0
37	37.02601608237558	40.0	35.0	41.0	31.0	41.0
38	36.87998024559452	39.0	35.0	41.0	31.0	41.0
39	36.75785516469098	39.0	35.0	41.0	31.0	41.0
40	36.60249175554437	39.0	35.0	41.0	30.0	41.0
41	36.43205130274874	39.0	35.0	41.0	30.0	41.0
42	36.28810318081152	39.0	35.0	41.0	30.0	41.0
43	35.440217973573716	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	5.0
11	2.0
12	5.0
13	4.0
14	3.0
15	2.0
16	1.0
17	16.0
18	47.0
19	98.0
20	186.0
21	403.0
22	722.0
23	1225.0
24	2162.0
25	3475.0
26	5372.0
27	7816.0
28	11590.0
29	16220.0
30	21829.0
31	28258.0
32	36270.0
33	46006.0
34	60817.0
35	79978.0
36	111592.0
37	178432.0
38	295935.0
39	454261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49339783112478	19.839601859203633	12.562906627412247	22.10409368225934
2	19.127724119307217	21.897156745190184	35.03978032396638	23.93533881153622
3	22.68094873981276	24.32558371626451	28.773847280540444	24.21962026338229
4	14.38952869745673	15.22586212716495	34.2889367991112	36.095672376267125
5	13.824781652178636	38.587501302528594	32.624782239233774	14.962934806058998
6	33.86801826328543	32.829737865203334	16.563026973716074	16.739216897795167
7	27.931569917533427	32.11375073932257	20.66155243796662	19.293126905177385
8	29.32421147487331	30.882475963761085	20.68327347816962	19.110039083195986
9	28.316384562210967	12.358611438475888	21.812694186833234	37.51230981247991
10	18.643697155864608	27.683759266298484	32.14567186259387	21.526871715243033
11	33.732261762016655	21.52283571115126	20.540619078998542	24.204283447833546
12	22.070704921136482	28.360046788294706	28.46659729631755	21.102650994251263
13	31.16815167156613	20.275563683007835	25.846129912367346	22.71015473305869
14	23.740289741064654	21.9711990748011	27.546681890963313	26.741829293170934
15	27.14946570644014	25.758218405059242	25.030343412580887	22.061972475919735
16	24.172435706454817	25.982326704991582	26.050351719411125	23.794885869142473
17	21.68016648883788	26.725685276803834	27.17103998285799	24.42310825150029
18	22.202425418313478	23.933504264221778	29.691487847224767	24.172582470239973
19	23.12762431993331	25.000403600409175	29.892187323424825	21.97978475623269
20	24.957108283788326	23.374334242779586	30.38516687776191	21.283390595670173
21	25.338327215729556	23.879421809392003	28.319393219806656	22.462857755071788
22	24.62740344043665	24.137139016124937	29.773822330696966	21.461635212741445
23	24.005491900840518	24.260273831870343	28.728790798497727	23.005443468791416
24	22.968899286287716	25.26538561450731	29.569967433116073	22.195747666088906
25	23.070092916152383	25.349921554756833	29.10384565146243	22.476139877628356
26	23.11801129200563	25.325485384528456	28.605435837074587	22.951067486391327
27	22.813843347271	25.09917562781878	29.41498487599194	22.67199614891828
28	22.268469121633423	24.948229074786422	29.123365234888098	23.659936568692057
29	21.79933868238409	25.19868147415416	30.200904945499268	22.80107489796248
30	21.940085152348146	26.529388714158447	29.408453887552522	22.12207224594088
31	23.086970751445257	26.53437868285373	28.2253910154146	22.153259550286407
32	21.436611987372444	26.488661763777817	29.500621544630135	22.574104704219604
33	21.87705010662389	25.394611127336663	29.716877982056662	23.011460783982788
34	22.12339312000728	24.842559149474514	30.286027940889422	22.74801978962879
35	21.428760124866628	25.5443101881952	29.52850666380967	23.498423023128506
36	21.739679203718406	26.778667003244948	28.599418521883212	22.88223527115343
37	21.998643902625165	26.14934389249846	28.774727863251375	23.077284341625
38	21.929151250354067	25.809512347971065	28.796669049132113	23.464667352542754
39	21.59922626132466	25.68109403596006	29.638212593213346	23.081467109501926
40	20.723119845839317	25.176666906380852	30.243393061301767	23.856820186478064
41	20.64027168911908	25.27272380376508	30.341064360322704	23.74594014679314
42	20.577897080428023	25.516131541445358	30.660275593035763	23.245695785090852
43	20.309539499271317	24.960777378417212	30.07035855860351	24.65932456370796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	245.5
2	373.0
3	1192.5
4	2012.0
5	2012.0
6	2905.0
7	3798.0
8	3699.5
9	3601.0
10	5064.0
11	6527.0
12	6527.0
13	11405.5
14	16284.0
15	21065.5
16	25847.0
17	24336.5
18	22826.0
19	22826.0
20	25031.0
21	27236.0
22	21698.5
23	16161.0
24	17074.0
25	17987.0
26	17987.0
27	20427.5
28	22868.0
29	26177.5
30	29487.0
31	34091.0
32	38695.0
33	38695.0
34	44412.0
35	50129.0
36	54812.5
37	59496.0
38	64412.5
39	69329.0
40	69329.0
41	71743.5
42	74158.0
43	76483.5
44	78809.0
45	78869.0
46	78929.0
47	78929.0
48	81596.0
49	84263.0
50	85829.0
51	87395.0
52	90485.5
53	93576.0
54	93576.0
55	95109.5
56	96643.0
57	94663.5
58	92684.0
59	86382.0
60	80080.0
61	80080.0
62	71254.5
63	62429.0
64	52784.5
65	43140.0
66	36534.0
67	29928.0
68	29928.0
69	24113.5
70	18299.0
71	15318.0
72	12337.0
73	9549.5
74	6762.0
75	6762.0
76	5407.0
77	4052.0
78	3313.0
79	2574.0
80	2055.0
81	1536.0
82	1536.0
83	1248.0
84	960.0
85	824.5
86	689.0
87	535.5
88	382.0
89	382.0
90	328.5
91	275.0
92	161.0
93	47.0
94	29.0
95	11.0
96	11.0
97	6.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1362734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.68166504550737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.28221262098012	60.12288239240385
2	9.585914005943664	13.359248978344082
3	2.1013544477826	4.392776303168227
4	0.7068685184704467	1.9702310134108683
5	0.33035488946545055	1.1509839376938567
6	0.18770008826916645	0.7847552807870534
7	0.12249229417989208	0.5974826909589295
8	0.08807052230318209	0.49095205084105764
9	0.06821013877272614	0.42776964384018096
>10	0.4477537651228521	5.962102887318984
>50	0.046878000254396736	2.232432321191609
>100	0.029139997969602257	3.802112509726987
>500	0.0018935444395562799	0.8504609508531769
>1k	7.363783931607754E-4	0.7221074665449616
>5k	1.0519691330868221E-4	0.4850456914148829
>10k+	3.1559073992604663E-4	2.648655881501279
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14083	1.0334371931719617	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11479	0.8423507448995915	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10571	0.7757199864390263	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6617	0.4855679831867407	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1749	0.12834493011842368	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1699	0.12467583548953795	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.11227429564390409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.9352757031086036E-4	0.0
3	0.0	0.0	0.0	6.604370331994359E-4	0.0
4	0.0	0.0	0.0	0.0013208740663988717	0.0
5	0.0	0.0	0.0	0.0017611654218651622	0.0
6	0.0	0.0	0.0	0.0019079292070205923	0.0
7	0.0	0.0	0.0	0.002421602455064598	0.0
8	0.0	0.0	0.0	0.0027885119179531736	0.0
9	0.0	0.0	0.0	0.00447629544724062	0.0
10	0.0	0.0	0.0	0.006384224654261213	0.0
11	0.0	0.0	0.0	0.006971279794882934	0.0
12	0.0	0.0	0.0	0.0075583349355046545	0.0
13	0.0	0.0	0.0	0.008292153861281806	0.0
14	0.0	0.0	0.0	0.009392882249947531	0.0
15	0.0	0.0	0.0	0.012034630382745276	0.0
16	0.0	0.0	0.0	0.018639000714739635	0.0
17	0.0	0.0	0.0	0.027004536468599155	0.0
18	0.0	0.0	0.0	0.0311139224529512	0.0
19	0.0	0.0	0.0	0.03639741871854669	0.0
20	0.0	0.0	0.0	0.039406076314233006	0.0
21	0.0	0.0	0.0	0.04777161206809252	0.0
22	0.0	0.0	0.0	0.065163120609011	0.0
23	0.0	0.0	0.0	0.09106692868894443	0.0
24	0.0	0.0	0.0	0.12607009144851453	0.0
25	0.0	0.0	0.0	0.1342154815246409	0.0
26	0.0	0.0	0.0	0.15013935221400507	0.0
27	0.0	0.0	0.0	0.16694380561430183	0.0
28	0.0	0.0	0.0	0.1968836177860096	7.338189257771509E-5
29	0.0	0.0	0.0	0.24979196233454218	7.338189257771509E-5
30	0.0	0.0	0.0	0.3424732926601963	7.338189257771509E-5
31	0.0	0.0	0.0	0.6393030481370539	7.338189257771509E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5025	0.0	24.003979	1
GTCTATG	105	4.798876E-7	17.619047	1
TATACTG	110	7.810213E-7	16.818182	5
GTATCAA	7680	0.0	15.705729	2
GTTAGTG	120	1.9360432E-6	15.416666	9
CTATGCT	125	2.9595358E-6	14.800001	4
TCTTGCG	75	0.004104925	14.8	2
GCTATAC	75	0.004104925	14.8	3
TCTATGG	535	0.0	14.523364	2
TCTTATA	1610	0.0	14.478261	37
AGTCGGT	315	0.0	14.095239	11
AGTTACG	80	0.006299414	13.875	20
TGCACCG	80	0.006299414	13.875	5
GCAGTCG	310	0.0	13.725806	9
TACGGGC	300	0.0	13.566667	4
GTGTAGA	150	1.3058889E-6	13.566667	1
ACACGCT	505	0.0	13.554455	9
TCGGTGA	330	0.0	13.454546	13
CAGTCGG	305	0.0	13.344262	10
CTCTATG	560	0.0	13.214285	1
>>END_MODULE