FastQCFastQC Report
Fri 10 Feb 2017
ERR1630665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548386
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89490.3511634422728739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.29767860912750266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70360.27609632135791046No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.20397223968425504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.018.977861
TCTAGCG3000.016.03333328
CTAGCGG3100.015.516129529
CGAACGA2300.015.28260916
TACACCG855.367335E-415.2352935
CGAATTA1954.1836756E-1115.17948615
TACGGGT1101.4531683E-515.1363644
CGAACTA1803.3360266E-914.38888824
TACCGTC3100.014.3225817
ACGCTTC3500.014.27142831
GTAAACG3500.014.27142827
TTCGGGC3250.014.23076935
AAACGCT3400.014.147058529
TTAACGG1051.6568792E-414.09523835
ACGAACG2650.013.96226415
CGCAATA3250.013.66153836
CAAGACG5200.013.5192314
AACACCG1102.4581992E-413.4545455
CGCTTCG3450.013.40579732
AAGACGG4850.013.3505155
Produced by FastQC (version 0.11.5)