FastQCFastQC Report
Fri 10 Feb 2017
ERR1630664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065812
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105390.9888235448653233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85230.7996719871797278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69900.6558379901896395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.4905180275695901No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15080.14148836755450306No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14070.13201202463473857No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.13088612250565765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1101.7535058E-920.1818183
TACACCG759.264735E-619.7333345
TCTAGAC500.007034555518.4999983
CTAGTAG500.007034555518.4999983
TAACGAA953.6058882E-617.52631613
CCTAGTA650.001579832217.0769232
AGTCGGT4100.016.69512411
GTATTAT904.4454697E-516.4444451
TTAACGA904.4454697E-516.44444512
TCGGTGA4500.016.44444513
CGGACCA1251.657263E-716.2799999
CAGTCGG4250.016.10588310
GGTATCA57450.016.0687541
GCCGTCT2003.6379788E-1215.72499936
GCAGTCG4500.015.6222229
ATGCCGT1902.7284841E-1115.57894834
CGAACGT855.363696E-415.2352944
TCGAACG855.363696E-415.2352943
CGGTGAT5000.015.1714
CCGCTTA1101.4515923E-515.13636425
Produced by FastQC (version 0.11.5)