FastQCFastQC Report
Fri 10 Feb 2017
ERR1630663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428703
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39750.2782243755350132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37270.26086597424377217No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28840.20186140856427123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27850.19493204675849354No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT27020.18912258181021527No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26430.18499296214818617No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC26340.18436302016584274No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT22660.15860539244335597No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22490.15741550203226284No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20680.1447466688318006No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA19350.13543752620383662No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19270.1348775777750869No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC19030.13319773248883776No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC18910.13235780984571321No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG18520.12962806125555837No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG18200.1273882675405595No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA17780.12444853828962352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.12115884127071896No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG17140.11996895085962583No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC17130.11989895730603212No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG16950.11863907334134527No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG16670.11667925384072128No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG16480.1153493763224407No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG16450.11513939566165955No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA16240.11366953103619157No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC15600.10918994360619386No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG15350.10744010476635103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG15230.10660018212322644No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG15120.10583025303369559No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC15090.10562027237291445No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG15000.10499033039057103No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14450.10114068494291675No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA14310.10016077519260476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG6100.022.7459015
CGCAAGA6500.021.9153842
GACGGAC6650.020.8646627
AGACGGA6700.020.7089566
CGGACCA6700.020.7089569
GCGCAAG6900.020.6449281
CAAGACG6950.020.4964054
ACGGACC6700.020.4328378
GAGCGAA7050.019.15602716
GGTATCA23250.019.0967731
CGAAAGC7300.018.519
GCGAAAG7300.018.518
AGCGAAA7300.018.517
GCTTTAT6550.018.0763361
TTGCCAA7700.018.01948228
AGAGCGA7600.018.01315915
GACCAGA7950.017.91823811
GCAAGAC8450.017.7337283
CAGAGCG7650.017.65359514
CTTGTAC953.6076271E-617.5263163
Produced by FastQC (version 0.11.5)