##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1428703
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.034553717602606	33.0	31.0	34.0	30.0	34.0
2	32.19114119589586	33.0	31.0	34.0	30.0	34.0
3	32.29505642530323	34.0	31.0	34.0	30.0	34.0
4	35.87456385266917	37.0	35.0	37.0	35.0	37.0
5	35.84592039073201	37.0	35.0	37.0	35.0	37.0
6	35.884427344241594	37.0	35.0	37.0	35.0	37.0
7	35.85704096652698	37.0	35.0	37.0	35.0	37.0
8	35.847484746654835	37.0	35.0	37.0	35.0	37.0
9	37.577589603997474	39.0	37.0	39.0	35.0	39.0
10	37.49420418379467	39.0	37.0	39.0	35.0	39.0
11	37.597226995393726	39.0	37.0	39.0	35.0	39.0
12	37.52234789175917	39.0	37.0	39.0	35.0	39.0
13	37.57231348992758	39.0	37.0	39.0	35.0	39.0
14	38.830441316354765	40.0	38.0	41.0	35.0	41.0
15	38.81048545428966	40.0	38.0	41.0	35.0	41.0
16	38.784868513609894	40.0	38.0	41.0	35.0	41.0
17	38.775566370337295	40.0	38.0	41.0	35.0	41.0
18	38.75216962517752	40.0	38.0	41.0	35.0	41.0
19	38.744630619519945	40.0	38.0	41.0	35.0	41.0
20	38.738771459148616	40.0	38.0	41.0	35.0	41.0
21	38.65620426358733	40.0	38.0	41.0	34.0	41.0
22	38.5938960021782	40.0	38.0	41.0	34.0	41.0
23	38.5561988740837	40.0	38.0	41.0	34.0	41.0
24	38.487276221859965	40.0	38.0	41.0	34.0	41.0
25	38.44291990707656	40.0	38.0	41.0	34.0	41.0
26	38.25791364615319	40.0	38.0	41.0	34.0	41.0
27	38.13584908829897	40.0	38.0	41.0	33.0	41.0
28	37.98505847611435	40.0	37.0	41.0	33.0	41.0
29	37.89858284052039	40.0	37.0	41.0	33.0	41.0
30	37.8098163159173	40.0	37.0	41.0	33.0	41.0
31	37.77902615169143	40.0	37.0	41.0	33.0	41.0
32	37.68622869833688	40.0	37.0	41.0	33.0	41.0
33	37.62117458982028	40.0	37.0	41.0	32.0	41.0
34	37.55504048077172	40.0	37.0	41.0	32.0	41.0
35	37.46139120586994	40.0	36.0	41.0	32.0	41.0
36	37.42553280842834	40.0	36.0	41.0	32.0	41.0
37	37.22941437093644	39.0	36.0	41.0	31.0	41.0
38	37.09654840789163	39.0	36.0	41.0	31.0	41.0
39	37.03718197554005	39.0	35.0	41.0	31.0	41.0
40	36.89372598783652	39.0	35.0	41.0	31.0	41.0
41	36.76346588479201	39.0	35.0	41.0	31.0	41.0
42	36.640380120990855	39.0	35.0	41.0	30.0	41.0
43	35.70141799940225	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	4.0
12	3.0
13	0.0
14	1.0
15	7.0
16	3.0
17	24.0
18	64.0
19	112.0
20	212.0
21	399.0
22	794.0
23	1293.0
24	2229.0
25	3405.0
26	5476.0
27	7940.0
28	11011.0
29	15618.0
30	20883.0
31	27170.0
32	35675.0
33	46588.0
34	62522.0
35	83951.0
36	121001.0
37	184770.0
38	325653.0
39	471892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78894493817119	18.57726903352201	11.584773042402794	25.049012985903996
2	21.042022029771058	19.849331876534173	31.15504062075883	27.953605472935944
3	21.972726311906673	21.755466321551786	27.865763563175832	28.406043803365712
4	14.925565355430765	14.139327767912574	32.82326697711141	38.11183989954525
5	15.863338986479345	35.405049194969145	32.925387571804634	15.806224246746876
6	37.63077420569566	32.58346906249934	14.252857311841579	15.532899419963423
7	30.744528428931694	29.02044721681133	20.03215503852095	20.20286931573602
8	30.05264215165783	29.091280693048173	19.14708655332844	21.708990601965557
9	26.921690512303815	12.79475160337733	19.368126195577386	40.915431688741464
10	19.34558827132021	24.363006167132006	30.235045352323052	26.05636020922473
11	38.639311319427485	20.24374555103475	19.429860509847042	21.687082619690727
12	24.392053491873398	24.685046507216686	25.089679240541944	25.83322076036797
13	31.351162557928415	17.709838923835115	24.328289364549523	26.610709153686944
14	24.23680779000254	20.069111634818434	22.622546463470712	33.07153411170831
15	27.830696792825382	26.354392760426766	20.694993991053423	25.11991645569443
16	28.206562175623624	24.11977856839385	22.10683396059223	25.566825295390295
17	26.178708940906542	24.418161087363856	22.695269765654583	26.70786020607502
18	25.740549295409892	22.085905888067707	25.801863648357987	26.37168116816441
19	26.589361119840866	23.1382589663492	24.542889599867852	25.729490313942087
20	27.1078033713095	22.02179179297587	24.015418179985623	26.854986655729007
21	28.26430685733844	22.909939994526503	23.917917159829578	24.907835988305475
22	27.721086887897624	22.686800545669744	24.02192758046984	25.57018498596279
23	26.082187830500807	22.50992683573843	24.38848382064012	27.019401513120638
24	25.80137369348283	23.855692890684768	24.42663030734869	25.916303108483707
25	26.502569113384656	23.384916249213447	23.776670168677466	26.335844468724428
26	26.31155670562741	24.659568853708574	23.999389656212664	25.02948478445135
27	27.34823122790391	22.167378384450792	24.031306716651397	26.453083670993898
28	25.305119398503393	23.394085404734223	25.129575566090363	26.171219630672017
29	25.586283503289348	23.9009787198599	24.957111450035452	25.5556263268153
30	25.24793466521733	24.138886808524934	24.912315575735473	25.700862950522257
31	25.43936703429614	24.870879392007996	23.843094051037898	25.846659522657962
32	25.151273567704415	23.142318592457634	23.583627947866002	28.12277989197195
33	24.148755899581648	23.880890569978504	24.917635085808598	27.052718444631253
34	27.108293326184658	22.706328747122388	24.246886861720036	25.93849106497292
35	26.14224229948422	22.824407872034985	25.201808913399077	25.831540915081717
36	24.148685906028057	24.667198151050286	25.04054376591916	26.1435721770025
37	25.901324488014655	22.83812660853935	25.477863488772684	25.782685414673306
38	25.115016906942873	22.502717499718276	25.755527915878947	26.626737677459904
39	25.634719042376197	22.166258487593293	25.43278764025833	26.766234829772177
40	24.54078979326004	22.695129778547397	26.233093932048856	26.530986496143704
41	23.235480012290868	23.221131333804156	27.336052349578605	26.207336304326372
42	24.816284420204898	22.904550490899787	27.605387543807215	24.673777545088097
43	24.253816223525813	21.87235555605329	27.019821474442203	26.854006745978698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	59.5
2	81.0
3	273.0
4	465.0
5	465.0
6	681.5
7	898.0
8	923.5
9	949.0
10	1339.5
11	1730.0
12	1730.0
13	3097.5
14	4465.0
15	6456.5
16	8448.0
17	7850.0
18	7252.0
19	7252.0
20	8439.0
21	9626.0
22	8591.0
23	7556.0
24	8617.5
25	9679.0
26	9679.0
27	11088.0
28	12497.0
29	14684.5
30	16872.0
31	20189.0
32	23506.0
33	23506.0
34	28955.0
35	34404.0
36	40817.5
37	47231.0
38	54281.0
39	61331.0
40	61331.0
41	65038.0
42	68745.0
43	70884.0
44	73023.0
45	76140.5
46	79258.0
47	79258.0
48	87781.5
49	96305.0
50	95782.5
51	95260.0
52	98688.5
53	102117.0
54	102117.0
55	101992.0
56	101867.0
57	100794.5
58	99722.0
59	103132.5
60	106543.0
61	106543.0
62	100623.0
63	94703.0
64	87775.5
65	80848.0
66	71590.0
67	62332.0
68	62332.0
69	55941.5
70	49551.0
71	41804.0
72	34057.0
73	24298.0
74	14539.0
75	14539.0
76	11055.5
77	7572.0
78	6142.5
79	4713.0
80	4202.5
81	3692.0
82	3692.0
83	2974.5
84	2257.0
85	2103.0
86	1949.0
87	1732.5
88	1516.0
89	1516.0
90	1239.0
91	962.0
92	534.5
93	107.0
94	70.5
95	34.0
96	34.0
97	18.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1428703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.355038657098994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.14353580971998	33.50827143193622
2	8.938936483573466	7.035843817287726
3	2.146855562481042	2.534687509579483
4	0.858732578659898	1.3518181531708235
5	0.4770789814081725	0.938773087790402
6	0.3050179073217702	0.7202394920253423
7	0.24272981650125766	0.6686848918146279
8	0.17703333381094732	0.5573722956579953
9	0.14318955369979067	0.5071707379033212
>10	1.0835966493722395	8.930562433394318
>50	0.19400637804946616	5.429854446119306
>100	0.2408340644438719	20.634249652361657
>500	0.03593737665164422	9.40928814823612
>1k	0.012515504306542762	7.773183902722735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3975	0.2782243755350132	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3727	0.26086597424377217	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2884	0.20186140856427123	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2785	0.19493204675849354	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2702	0.18912258181021527	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2643	0.18499296214818617	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2634	0.18436302016584274	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2266	0.15860539244335597	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2249	0.15741550203226284	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2068	0.1447466688318006	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1935	0.13543752620383662	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1927	0.1348775777750869	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1903	0.13319773248883776	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	1891	0.13235780984571321	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1852	0.12962806125555837	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1820	0.1273882675405595	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1778	0.12444853828962352	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1731	0.12115884127071896	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1714	0.11996895085962583	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1713	0.11989895730603212	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	1695	0.11863907334134527	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1667	0.11667925384072128	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	1648	0.1153493763224407	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	1645	0.11513939566165955	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1624	0.11366953103619157	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	1560	0.10918994360619386	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1535	0.10744010476635103	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1523	0.10660018212322644	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	1512	0.10583025303369559	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1509	0.10562027237291445	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1500	0.10499033039057103	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1445	0.10114068494291675	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	1431	0.10016077519260476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.099161967182823E-4	0.0
3	0.0	0.0	0.0	9.799097503119963E-4	0.0
4	0.0	0.0	0.0	0.0013998710718742804	0.0
5	6.999355359371402E-5	6.999355359371402E-5	0.0	0.0014698646254679944	0.0
6	6.999355359371402E-5	6.999355359371402E-5	0.0	0.0014698646254679944	0.0
7	6.999355359371402E-5	6.999355359371402E-5	0.0	0.0016798452862491365	0.0
8	6.999355359371402E-5	6.999355359371402E-5	0.0	0.0017498388398428505	0.0
9	6.999355359371402E-5	6.999355359371402E-5	0.0	0.0025197679293737047	0.0
10	6.999355359371402E-5	6.999355359371402E-5	0.0	0.003639664786873129	0.0
11	6.999355359371402E-5	6.999355359371402E-5	0.0	0.003919639001247985	0.0
12	6.999355359371402E-5	6.999355359371402E-5	0.0	0.004689568090778839	0.0
13	6.999355359371402E-5	6.999355359371402E-5	0.0	0.0050395358587474095	0.0
14	6.999355359371402E-5	6.999355359371402E-5	0.0	0.005319510073122266	0.0
15	6.999355359371402E-5	6.999355359371402E-5	0.0	0.00608943916265312	0.0
16	6.999355359371402E-5	6.999355359371402E-5	0.0	0.007909271556089684	0.0
17	1.3998710718742804E-4	6.999355359371402E-5	0.0	0.009869091056713677	0.0
18	1.3998710718742804E-4	1.3998710718742804E-4	0.0	0.010918994360619386	0.0
19	1.3998710718742804E-4	1.3998710718742804E-4	0.0	0.012318865432493668	0.0
20	1.3998710718742804E-4	1.3998710718742804E-4	0.0	0.013438762289993092	0.0
21	1.3998710718742804E-4	1.3998710718742804E-4	0.0	0.015468575344210799	0.0
22	1.3998710718742804E-4	1.3998710718742804E-4	0.0	0.01952820145264621	0.0
23	1.3998710718742804E-4	1.3998710718742804E-4	0.0	0.024987698632955906	0.0
24	1.3998710718742804E-4	2.799742143748561E-4	0.0	0.03471680258248215	0.0
25	1.3998710718742804E-4	2.799742143748561E-4	0.0	0.03863644158373014	0.0
26	1.3998710718742804E-4	2.799742143748561E-4	0.0	0.04507584851435183	0.0
27	1.3998710718742804E-4	2.799742143748561E-4	0.0	0.057534701054032925	0.0
28	1.3998710718742804E-4	2.799742143748561E-4	0.0	0.09799097503119962	0.0
29	1.3998710718742804E-4	3.499677679685701E-4	0.0	0.15622561162116969	0.0
30	1.3998710718742804E-4	3.499677679685701E-4	0.0	0.23748812734347166	0.0
31	1.3998710718742804E-4	3.499677679685701E-4	0.0	0.4922646624245907	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	610	0.0	22.745901	5
CGCAAGA	650	0.0	21.915384	2
GACGGAC	665	0.0	20.864662	7
AGACGGA	670	0.0	20.708956	6
CGGACCA	670	0.0	20.708956	9
GCGCAAG	690	0.0	20.644928	1
CAAGACG	695	0.0	20.496405	4
ACGGACC	670	0.0	20.432837	8
GAGCGAA	705	0.0	19.156027	16
GGTATCA	2325	0.0	19.096773	1
CGAAAGC	730	0.0	18.5	19
GCGAAAG	730	0.0	18.5	18
AGCGAAA	730	0.0	18.5	17
GCTTTAT	655	0.0	18.076336	1
TTGCCAA	770	0.0	18.019482	28
AGAGCGA	760	0.0	18.013159	15
GACCAGA	795	0.0	17.918238	11
GCAAGAC	845	0.0	17.733728	3
CAGAGCG	765	0.0	17.653595	14
CTTGTAC	95	3.6076271E-6	17.526316	3
>>END_MODULE