FastQCFastQC Report
Fri 10 Feb 2017
ERR1630662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708830
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69450.4064184266427907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58950.34497287617843786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53010.31021225048717543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.24923485659778916No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG31380.18363441653060866No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT29860.17473944160624522No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG26980.1578858049074513No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC20870.12213034649438505No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA20750.1214281116319353No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19400.11352796942937565No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT18540.1084952862484858No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA17200.10065366361779698No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC17100.10006846789908884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1650.019.06060628
GGTATCA33500.017.782091
CTAGCGG1800.017.47222129
ACTACAC1950.017.0769233
TTCGGAA2350.016.53191631
TTTCGGA2500.016.2830
GCTATAC700.002593185815.8571433
TGCGTTA1902.7284841E-1115.57894837
AATGCGT2055.456968E-1215.34146335
CGAACGA1455.3498297E-815.31034616
GACGGAC2300.015.2826097
CCGTCCA855.366049E-415.2352949
GTCCTAT2450.015.102041
ACGGACC2450.015.102048
GTTTTCG2700.015.07407528
AAATGCG2109.094947E-1214.9761934
TGTAATA1904.5656634E-1014.6052642
GTATTAG3050.014.5573771
ATGCGTT2059.276846E-1114.43902436
GCGTTAT2450.014.3469381
Produced by FastQC (version 0.11.5)