##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630662.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1708830
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02077444801414	33.0	31.0	34.0	30.0	34.0
2	32.195324871403244	33.0	31.0	34.0	30.0	34.0
3	32.29012599263824	34.0	31.0	34.0	30.0	34.0
4	35.867482429498544	37.0	35.0	37.0	35.0	37.0
5	35.84756821919091	37.0	35.0	37.0	35.0	37.0
6	35.891263027919685	37.0	35.0	37.0	35.0	37.0
7	35.86544536320172	37.0	35.0	37.0	35.0	37.0
8	35.86506732676744	37.0	35.0	37.0	35.0	37.0
9	37.59429609732975	39.0	37.0	39.0	35.0	39.0
10	37.50744017836765	39.0	37.0	39.0	35.0	39.0
11	37.61104849516921	39.0	37.0	39.0	35.0	39.0
12	37.5489568886314	39.0	37.0	39.0	35.0	39.0
13	37.588097704277196	39.0	37.0	39.0	35.0	39.0
14	38.87612284428527	40.0	38.0	41.0	35.0	41.0
15	38.861312125840485	40.0	38.0	41.0	35.0	41.0
16	38.83097850576125	40.0	38.0	41.0	35.0	41.0
17	38.8321904460947	40.0	38.0	41.0	35.0	41.0
18	38.81589450091583	40.0	38.0	41.0	35.0	41.0
19	38.859022254993185	40.0	38.0	41.0	35.0	41.0
20	38.841124043936496	40.0	38.0	41.0	35.0	41.0
21	38.766930589935804	40.0	38.0	41.0	35.0	41.0
22	38.72065331250037	40.0	38.0	41.0	34.0	41.0
23	38.69781312359919	40.0	38.0	41.0	34.0	41.0
24	38.65094538368357	40.0	38.0	41.0	34.0	41.0
25	38.61135104135578	40.0	38.0	41.0	34.0	41.0
26	38.428284849868035	40.0	38.0	41.0	34.0	41.0
27	38.31832657432278	40.0	38.0	41.0	34.0	41.0
28	38.236032255988015	40.0	38.0	41.0	34.0	41.0
29	38.142373436795936	40.0	38.0	41.0	33.0	41.0
30	38.06403504151964	40.0	37.0	41.0	33.0	41.0
31	38.006258668211586	40.0	37.0	41.0	33.0	41.0
32	37.905080084034104	40.0	37.0	41.0	33.0	41.0
33	37.87489568886314	40.0	37.0	41.0	33.0	41.0
34	37.789127648742124	40.0	37.0	41.0	33.0	41.0
35	37.73645476729692	40.0	37.0	41.0	33.0	41.0
36	37.68740249176337	40.0	37.0	41.0	33.0	41.0
37	37.50169414160566	40.0	37.0	41.0	32.0	41.0
38	37.38996564901131	40.0	36.0	41.0	32.0	41.0
39	37.317081277833374	40.0	36.0	41.0	32.0	41.0
40	37.21873504093444	40.0	36.0	41.0	31.0	41.0
41	37.09151817325304	40.0	36.0	41.0	31.0	41.0
42	37.00180591398793	40.0	36.0	41.0	31.0	41.0
43	36.18611915755224	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	2.0
10	6.0
11	8.0
12	3.0
13	3.0
14	2.0
15	4.0
16	7.0
17	26.0
18	51.0
19	112.0
20	225.0
21	393.0
22	730.0
23	1287.0
24	2278.0
25	3660.0
26	5632.0
27	8500.0
28	12033.0
29	17541.0
30	23406.0
31	31124.0
32	40630.0
33	53199.0
34	70381.0
35	94488.0
36	133622.0
37	206139.0
38	375880.0
39	627454.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.020721780399455	20.22208177524973	12.664922783424917	24.092273660925898
2	18.035673531012446	22.008274667462533	35.932129000544236	24.023922800980788
3	20.43591229086568	24.57664015730061	30.454814112579953	24.532633439253758
4	14.621758747213006	15.933299391981649	34.91090395182669	34.53403790897866
5	13.586781599105821	38.16687441114681	34.59794128146159	13.648402708285786
6	33.48068561530404	35.13725765582299	16.303318644920793	15.078738083952178
7	27.112702843465996	31.749618159793542	22.490768537537377	18.64691045920308
8	25.979178736328368	34.4335012845046	20.952991227916176	18.634328751250855
9	25.96214954091396	14.620412797059975	21.136098968299947	38.28133869372612
10	16.739874651077052	28.018117659451203	33.48232416331642	21.759683526155325
11	33.591170566996134	22.742051579150647	22.674110356208637	20.992667497644586
12	20.40273169361493	27.185852308304515	29.47408460759701	22.937331390483546
13	28.46614350169414	21.038488322419433	27.038500026333807	23.45686814955262
14	22.018340033824312	22.6270606204245	26.213725180386582	29.140874165364604
15	24.893757717268542	28.72954009468467	24.12551277774852	22.251189410298274
16	24.149798400074904	27.75624257532932	25.311353382138655	22.78260564245712
17	22.73134249749829	27.73049396370616	26.642322524768407	22.89584101402714
18	21.711580438077515	26.03126115529339	28.753357560436086	23.50380084619301
19	22.95348279231989	26.848194378609925	28.60384005430616	21.59448277476402
20	23.347319511010458	25.677334784618715	28.913174511215278	22.06217119315555
21	23.12986078193852	27.038675585049422	27.53679418081377	22.29466945219829
22	23.17966093760058	26.356805533610718	27.76074858236337	22.702784946425332
23	22.455832353130504	26.856913794818677	28.123862525821764	22.563391326229056
24	23.489112433653435	26.947268013787212	27.644294634340454	21.919324918218898
25	23.120848767870413	26.217704511273794	28.05252716771124	22.60891955314455
26	22.89074981127438	26.969856568529345	27.711065465845053	22.428328154351224
27	23.11558200640204	26.46073629325328	27.437779065208357	22.98590263513632
28	22.23445281274322	26.708976317129263	28.269693298923826	22.78687757120369
29	22.131985042397428	27.389324859699325	28.092847152730233	22.385842945173014
30	21.73381787538842	27.996582457002745	28.050478982695765	22.21912068491307
31	22.543962828367945	27.19855105540048	27.95433132611202	22.303154790119557
32	21.891001445433425	27.076069591474866	27.90581860103111	23.12711036206059
33	21.901827566229525	26.812204841909377	28.349572514527484	22.936395077333614
34	22.40369141459361	26.946039102777924	27.851102801331905	22.79916668129656
35	22.587618428983575	26.746545882270322	28.109115593710314	22.556720095035786
36	22.146790494080744	27.2073289911811	27.817044410503094	22.828836104235062
37	23.043661452572813	25.642047482780615	28.00506779492401	23.30922326972256
38	22.050174680922037	26.08562583756137	28.946706225897252	22.91749325561934
39	22.18740307695909	26.081705026246027	28.884968077573543	22.84592381922134
40	21.86185869864176	25.36829292556896	29.762234979488888	23.007613396300393
41	21.12679435637249	26.33556292902161	29.72958105838498	22.808061656220925
42	22.10044299315906	25.410836654319034	29.424635569366174	23.064084783155728
43	20.945091085713617	25.605882387364453	29.45828432319189	23.990742203730036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	419.0
1	400.5
2	382.0
3	964.0
4	1546.0
5	1546.0
6	2070.0
7	2594.0
8	2603.0
9	2612.0
10	3840.5
11	5069.0
12	5069.0
13	8420.0
14	11771.0
15	15625.5
16	19480.0
17	19682.5
18	19885.0
19	19885.0
20	23435.5
21	26986.0
22	27827.0
23	28668.0
24	30754.5
25	32841.0
26	32841.0
27	35133.0
28	37425.0
29	46636.5
30	55848.0
31	58104.0
32	60360.0
33	60360.0
34	65503.5
35	70647.0
36	74654.5
37	78662.0
38	90074.5
39	101487.0
40	101487.0
41	106377.5
42	111268.0
43	105952.0
44	100636.0
45	105276.0
46	109916.0
47	109916.0
48	114994.0
49	120072.0
50	122095.0
51	124118.0
52	127345.0
53	130572.0
54	130572.0
55	113718.0
56	96864.0
57	90127.0
58	83390.0
59	75568.0
60	67746.0
61	67746.0
62	63093.0
63	58440.0
64	52780.5
65	47121.0
66	40614.0
67	34107.0
68	34107.0
69	29715.5
70	25324.0
71	21518.0
72	17712.0
73	14022.5
74	10333.0
75	10333.0
76	7943.5
77	5554.0
78	4618.0
79	3682.0
80	2942.5
81	2203.0
82	2203.0
83	1689.5
84	1176.0
85	1003.5
86	831.0
87	755.0
88	679.0
89	679.0
90	512.5
91	346.0
92	195.5
93	45.0
94	26.5
95	8.0
96	8.0
97	6.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1708830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.3818516569568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09802256823868	43.88159652647276
2	7.721290124627231	7.780257873185892
3	1.9071958654436192	2.882641775206253
4	0.8341216363810695	1.680983701920364
5	0.4713716642632995	1.18742886321032
6	0.3062908094076302	0.925889887407867
7	0.2120530090677748	0.7478536272384763
8	0.1605882719241796	0.6472587595144845
9	0.12588481896485942	0.5708079247455369
>10	0.8585630190621285	8.667076585525392
>50	0.131543772028299	4.722572786188422
>100	0.15350758251939958	16.5615591070696
>500	0.014092796587549753	4.728154311753778
>1k	0.005124653304563547	3.951414604835034
>5k	3.4940817985660547E-4	1.06450366572584
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6945	0.4064184266427907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5895	0.34497287617843786	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5301	0.31021225048717543	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4259	0.24923485659778916	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3138	0.18363441653060866	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2986	0.17473944160624522	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2698	0.1578858049074513	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2087	0.12213034649438505	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2075	0.1214281116319353	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1940	0.11352796942937565	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1854	0.1084952862484858	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1720	0.10065366361779698	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1710	0.10006846789908884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.851957187081219E-5	0.0
2	0.0	0.0	0.0	0.0019896654436076145	0.0
3	0.0	0.0	0.0	0.0023407828748324877	0.0
4	0.0	0.0	0.0	0.0038622917434736046	0.0
5	0.0	1.1703914374162439E-4	0.0	0.00427192874656929	0.0
6	0.0	1.1703914374162439E-4	0.0	0.004447487462181727	0.0
7	0.0	1.1703914374162439E-4	0.0	0.005383800612114722	0.0
8	0.0	1.1703914374162439E-4	0.0	0.0056763984714687825	0.0
9	5.851957187081219E-5	1.1703914374162439E-4	0.0	0.009304611927459138	0.0
10	5.851957187081219E-5	1.1703914374162439E-4	0.0	0.014044697248994927	0.0
11	5.851957187081219E-5	1.1703914374162439E-4	0.0	0.015507686545765231	0.0
12	5.851957187081219E-5	1.7555871561243658E-4	0.0	0.017029195414406346	0.0
13	5.851957187081219E-5	1.7555871561243658E-4	0.0	0.01790698899246853	0.0
14	5.851957187081219E-5	1.7555871561243658E-4	0.0	0.01942849786110965	0.0
15	5.851957187081219E-5	1.7555871561243658E-4	0.0	0.02165224159220051	0.0
16	5.851957187081219E-5	1.7555871561243658E-4	0.0	0.02510489633257843	0.0
17	5.851957187081219E-5	1.7555871561243658E-4	0.0	0.03013757951346828	0.0
18	5.851957187081219E-5	2.9259785935406097E-4	0.0	0.03265392110391321	0.0
19	5.851957187081219E-5	2.9259785935406097E-4	0.0	0.03634065413177437	0.0
20	5.851957187081219E-5	2.9259785935406097E-4	0.0	0.039676269728410665	0.0
21	5.851957187081219E-5	2.9259785935406097E-4	0.0	0.045586746487362696	0.0
22	5.851957187081219E-5	2.9259785935406097E-4	0.0	0.05442320183985534	0.0
23	5.851957187081219E-5	3.5111743122487316E-4	0.0	0.06688787064833834	0.0
24	1.1703914374162439E-4	4.0963700309568535E-4	0.0	0.08824751438118479	0.0
25	1.1703914374162439E-4	4.0963700309568535E-4	0.0	0.09538690214942387	0.0
26	1.1703914374162439E-4	4.0963700309568535E-4	0.0	0.10720785566732793	0.0
27	1.1703914374162439E-4	4.0963700309568535E-4	0.0	0.12780674496585384	0.0
28	1.1703914374162439E-4	4.6815657496649754E-4	0.0	0.16800969084110182	0.0
29	1.7555871561243658E-4	5.851957187081219E-4	0.0	0.22658778228378482	0.0
30	1.7555871561243658E-4	5.851957187081219E-4	0.0	0.3097440939122089	0.0
31	1.7555871561243658E-4	5.851957187081219E-4	0.0	0.5742525587682801	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	165	0.0	19.060606	28
GGTATCA	3350	0.0	17.78209	1
CTAGCGG	180	0.0	17.472221	29
ACTACAC	195	0.0	17.076923	3
TTCGGAA	235	0.0	16.531916	31
TTTCGGA	250	0.0	16.28	30
GCTATAC	70	0.0025931858	15.857143	3
TGCGTTA	190	2.7284841E-11	15.578948	37
AATGCGT	205	5.456968E-12	15.341463	35
CGAACGA	145	5.3498297E-8	15.310346	16
GACGGAC	230	0.0	15.282609	7
CCGTCCA	85	5.366049E-4	15.235294	9
GTCCTAT	245	0.0	15.10204	1
ACGGACC	245	0.0	15.10204	8
GTTTTCG	270	0.0	15.074075	28
AAATGCG	210	9.094947E-12	14.97619	34
TGTAATA	190	4.5656634E-10	14.605264	2
GTATTAG	305	0.0	14.557377	1
ATGCGTT	205	9.276846E-11	14.439024	36
GCGTTAT	245	0.0	14.346938	1
>>END_MODULE