FastQCFastQC Report
Fri 10 Feb 2017
ERR1630661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246793
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14300.11469425959241028No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13010.10434771449631174No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12880.10330503940910801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.020.9433961
TCTAGCG1800.019.52777928
CCTAGAC609.237416E-418.53
GTATACC609.237416E-418.54
ATTGCCG2500.017.0211
CGTATGC1005.8785936E-616.65000231
AAGACGG2150.016.3488375
TATACTG1251.6579179E-716.285
CTAGCGG2050.016.24390229
TATACAG1403.4733603E-815.8571435
CAAGACG2600.015.6538464
TAGATTG957.0604416E-515.5789485
TAATGGT957.0604416E-515.5789484
GTAGACA1101.45198555E-515.1363644
AAGACCG750.004104663614.8000015
CGCAATA2251.8189894E-1214.836
CCGCTAC3300.014.5757591
AGTCGGT1651.6449121E-814.57575911
GTTCAAA3950.014.5189871
CTTATAC6050.014.371901537
Produced by FastQC (version 0.11.5)