FastQCFastQC Report
Fri 10 Feb 2017
ERR1630660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443547
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70310.48706415516779156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.3943065241381126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53420.3700606907845744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.28783267881128916No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC27080.1875934763468041No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT20990.1454057263116476No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA18340.12704816677254013No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG17940.12427721438927865No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA17850.12365375010304479No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG17780.12316883343597403No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG15240.10557328580226345No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG14780.10238669056151271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC2200.020.1818187
CGTATGC1950.018.9743631
CTCGTAT2000.018.49999829
TCGTATG2000.018.49999830
ACGGACC2550.018.1372558
TCTCGTA2250.018.08888828
ATGCCGT2100.017.61904734
GCGCAAG2750.017.4909081
CTTATGG752.0677279E-417.2666663
ACGAACG752.0677279E-417.26666615
GCGTTAT1301.3938916E-817.0769231
CGAATTA650.001580198917.07692315
CGGACCA2650.016.7547179
GCGAAAG2550.016.68627418
CGAAAGC2700.016.44444519
CGTTATT1251.6584636E-716.2799992
ATGGGTA2450.015.8571435
TTTCGGA1751.3096724E-1015.85714230
CAAGACG3550.015.6338034
GGTATCA38050.015.4612351
Produced by FastQC (version 0.11.5)