##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1443547
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85446265345015	31.0	31.0	34.0	30.0	34.0
2	32.01462439394076	33.0	31.0	34.0	30.0	34.0
3	32.11581680402509	34.0	31.0	34.0	30.0	34.0
4	35.72571589286667	37.0	35.0	37.0	33.0	37.0
5	35.694262812364265	37.0	35.0	37.0	33.0	37.0
6	35.734167297635615	37.0	35.0	37.0	33.0	37.0
7	35.70936796654352	37.0	35.0	37.0	33.0	37.0
8	35.71089891773527	37.0	35.0	37.0	33.0	37.0
9	37.396855107592614	39.0	37.0	39.0	34.0	39.0
10	37.2934757233398	39.0	37.0	39.0	34.0	39.0
11	37.4051028473614	39.0	37.0	39.0	34.0	39.0
12	37.335341350160405	39.0	37.0	39.0	34.0	39.0
13	37.39135199615946	39.0	37.0	39.0	34.0	39.0
14	38.62357581706726	40.0	38.0	41.0	34.0	41.0
15	38.5911231154926	40.0	38.0	41.0	34.0	41.0
16	38.561633254753744	40.0	38.0	41.0	34.0	41.0
17	38.56416313427966	40.0	38.0	41.0	34.0	41.0
18	38.548557130457134	40.0	38.0	41.0	34.0	41.0
19	38.56470901189916	40.0	38.0	41.0	34.0	41.0
20	38.5452188255734	40.0	38.0	41.0	34.0	41.0
21	38.46887978015264	40.0	38.0	41.0	34.0	41.0
22	38.40646615593396	40.0	38.0	41.0	34.0	41.0
23	38.39536641342471	40.0	38.0	41.0	34.0	41.0
24	38.32983269682248	40.0	38.0	41.0	34.0	41.0
25	38.28734291297755	40.0	38.0	41.0	34.0	41.0
26	38.06218086421848	40.0	37.0	41.0	33.0	41.0
27	37.92403918957956	40.0	37.0	41.0	33.0	41.0
28	37.82414912711536	40.0	37.0	41.0	33.0	41.0
29	37.720892357505505	40.0	37.0	41.0	33.0	41.0
30	37.621746988494316	40.0	37.0	41.0	32.0	41.0
31	37.55572696974882	40.0	37.0	41.0	32.0	41.0
32	37.45613409192773	40.0	36.0	41.0	32.0	41.0
33	37.4012775475963	40.0	36.0	41.0	32.0	41.0
34	37.312636166331956	40.0	36.0	41.0	32.0	41.0
35	37.23654858483998	39.0	36.0	41.0	31.0	41.0
36	37.17593192324185	39.0	36.0	41.0	31.0	41.0
37	36.95326026793724	39.0	35.0	41.0	31.0	41.0
38	36.825831095212	39.0	35.0	41.0	31.0	41.0
39	36.72396950012712	39.0	35.0	41.0	30.0	41.0
40	36.581211418817674	39.0	35.0	41.0	30.0	41.0
41	36.43894033238959	39.0	35.0	41.0	30.0	41.0
42	36.32274944979277	39.0	35.0	41.0	30.0	41.0
43	35.42947129535789	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	1.0
13	2.0
14	3.0
15	9.0
16	7.0
17	19.0
18	38.0
19	99.0
20	223.0
21	467.0
22	881.0
23	1550.0
24	2624.0
25	4230.0
26	6510.0
27	9286.0
28	13289.0
29	18008.0
30	24213.0
31	31464.0
32	40458.0
33	51734.0
34	67946.0
35	89454.0
36	125151.0
37	193320.0
38	345129.0
39	417426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64738591815854	18.976659575337692	11.781258247912954	24.59469625859082
2	19.639332837794683	21.02460120799669	32.79158905113585	26.544476903072777
3	22.175308458955616	23.28327376940273	27.833731773194774	26.707685998446884
4	15.501677465299016	14.882923798116723	33.03411665848081	36.58128207810345
5	14.974573048193097	36.75398168539022	33.330747111108955	14.940698155307725
6	36.328779042178745	32.793736539232874	15.023549631567242	15.853934787021137
7	29.522003786506435	29.702323512847173	21.173747720025744	19.60192498062065
8	28.806405333529145	31.0856522163809	19.807114004601168	20.300828445488786
9	27.124922153556486	13.64617847565753	19.95910074282306	39.26979862796293
10	18.527488194011003	26.42068460535057	31.52180012150626	23.530027079132164
11	35.476364815277925	21.825752815807174	20.818234529253292	21.87964783966161
12	23.258404471762955	25.396055687830042	27.61953715396866	23.726002686438335
13	30.9171783114786	19.59271156394631	24.991496639873866	24.498613484701227
14	23.469758864796226	20.68079529104352	25.16689792573432	30.682547918425936
15	26.512680224474856	27.09284837972023	22.377726530552867	24.01674486525205
16	25.474958556943417	25.525597711747523	24.486975484691527	24.512468246617534
17	24.37405917507362	25.86420809298208	25.0776732589933	24.684059472951002
18	23.659707650668803	24.399967579857115	27.276839617968797	24.663485151505284
19	25.023431866090956	24.613469460987414	27.299215058463634	23.063883614457996
20	25.348187485409202	23.999564960475826	26.56040987927653	24.091837674838438
21	25.22467228292532	24.320094877409602	26.730892724656698	23.72434011500838
22	25.362042247325512	24.430863698930484	26.62414178409155	23.58295226965246
23	24.56996550857021	24.565601258566574	26.304997343349402	24.559435889513814
24	24.19006793682506	25.227304687689422	26.782640260414105	23.799987115071417
25	24.60508733002805	25.03098271133534	26.09759155746228	24.266338401174327
26	24.468479377533257	25.436303771196922	26.324671105270557	23.770545745999264
27	24.548975544267005	24.615755496703606	26.355428676724763	24.47984028230463
28	24.058516972429718	25.20402868767002	26.396092402949122	24.341361936951134
29	24.129245532012465	25.60062124752433	26.74731061752752	23.522822602935687
30	24.195956210639487	25.23665665198293	26.214040831368845	24.353346306008742
31	23.406858245696192	26.04591329551445	26.738928486568152	23.808299972221203
32	23.517973436264977	25.292560616315228	26.414242141059486	24.775223806360305
33	23.060489197788502	25.886514259667333	26.734564236564516	24.318432305979645
34	24.78083498493641	24.700061722964335	26.353350462437312	24.165752829661937
35	23.71097026975914	24.918482044574926	26.771902819928968	24.598644865736965
36	23.21711728125236	25.672666009489127	26.87394314144257	24.236273567815942
37	23.81307986508233	24.41943352034953	27.405688903790455	24.361797710777687
38	23.790912246016234	24.73802377061502	26.918278379574755	24.55278560379399
39	23.538201388662785	24.57592305619422	27.324915641818382	24.56095991332461
40	22.882386233354367	24.374405544121526	27.75579873741555	24.987409485108554
41	21.86828693489024	24.820598151636215	28.36963396411755	24.941480949355995
42	22.579036221196816	24.624622544330045	28.61687219051406	24.17946904395908
43	22.933025388158473	24.32584460360487	28.06898563053368	24.672144377702978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	446.0
1	424.0
2	402.0
3	864.5
4	1327.0
5	1327.0
6	1896.0
7	2465.0
8	2335.5
9	2206.0
10	3231.0
11	4256.0
12	4256.0
13	7479.0
14	10702.0
15	13880.5
16	17059.0
17	16552.5
18	16046.0
19	16046.0
20	18087.5
21	20129.0
22	18013.5
23	15898.0
24	17300.5
25	18703.0
26	18703.0
27	20993.0
28	23283.0
29	26221.0
30	29159.0
31	32906.5
32	36654.0
33	36654.0
34	41255.5
35	45857.0
36	50995.5
37	56134.0
38	60703.0
39	65272.0
40	65272.0
41	68370.0
42	71468.0
43	74245.0
44	77022.0
45	80864.5
46	84707.0
47	84707.0
48	90023.0
49	95339.0
50	95344.5
51	95350.0
52	95467.0
53	95584.0
54	95584.0
55	91864.0
56	88144.0
57	88009.5
58	87875.0
59	89674.5
60	91474.0
61	91474.0
62	84591.0
63	77708.0
64	72130.5
65	66553.0
66	57709.5
67	48866.0
68	48866.0
69	44704.0
70	40542.0
71	33922.5
72	27303.0
73	20253.5
74	13204.0
75	13204.0
76	9969.5
77	6735.0
78	5471.5
79	4208.0
80	3307.5
81	2407.0
82	2407.0
83	1900.0
84	1393.0
85	1104.0
86	815.0
87	661.5
88	508.0
89	508.0
90	392.0
91	276.0
92	165.5
93	55.0
94	33.0
95	11.0
96	11.0
97	6.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1443547.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.98636707805974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.12698033982328	51.393040447277095
2	7.809049016488869	9.212548636343476
3	2.003159459185202	3.5447729752615795
4	0.8463452665787754	1.9969133027677592
5	0.47577295532497105	1.4032059094306029
6	0.29368400205203826	1.0394011409997108
7	0.20605641247632236	0.8508163429581508
8	0.1570613972105321	0.7411584983722715
9	0.12670510342292193	0.6726486367051197
>10	0.7731706794636974	8.829631629896879
>50	0.08985412037285517	3.762777286245246
>100	0.07978670205875865	9.308986908806204
>500	0.009546077989322546	3.6563905868451005
>1k	0.0024749091083428824	2.3318813971354273
>5k	3.535584440489832E-4	1.255826300955506
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7031	0.48706415516779156	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5692	0.3943065241381126	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5342	0.3700606907845744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4155	0.28783267881128916	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2708	0.1875934763468041	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2099	0.1454057263116476	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1834	0.12704816677254013	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1794	0.12427721438927865	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1785	0.12365375010304479	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1778	0.12316883343597403	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1524	0.10557328580226345	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1478	0.10238669056151271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0018011190491199801	0.0
3	0.0	0.0	0.0	0.00270167857367997	0.0
4	0.0	0.0	0.0	0.004641345241963026	0.0
5	0.0	0.0	0.0	0.005264809528196865	0.0
6	0.0	0.0	0.0	0.005472630956941478	0.0
7	0.0	0.0	0.0	0.006373190481501468	0.0
8	0.0	0.0	0.0	0.0072044761964799205	0.0
9	0.0	6.92738095815377E-5	0.0	0.010044702389322967	0.0
10	0.0	6.92738095815377E-5	0.0	0.014824595250449067	0.0
11	0.0	6.92738095815377E-5	0.0	0.016625714299569046	0.0
12	0.0	6.92738095815377E-5	0.0	0.017872642872036725	0.0
13	0.0	1.385476191630754E-4	0.0	0.01960448811157517	0.0
14	0.0	1.385476191630754E-4	0.0	0.021197785731950535	0.0
15	0.0	1.385476191630754E-4	0.0	0.02355309525772282	0.0
16	0.0	2.078214287446131E-4	0.0	0.027640250023033542	0.0
17	0.0	2.078214287446131E-4	0.0	0.03276651193206733	0.0
18	0.0	2.078214287446131E-4	0.0	0.034914000029095	0.0
19	0.0	2.770952383261508E-4	0.0	0.03948607146147649	0.0
20	0.0	2.770952383261508E-4	0.0	0.04253411908306415	0.0
21	0.0	2.770952383261508E-4	0.0	0.047660380992097934	0.0
22	0.0	2.770952383261508E-4	0.0	0.05583469052271939	0.0
23	0.0	3.4636904790768847E-4	0.0	0.06865034529530385	0.0
24	0.0	4.156428574892262E-4	0.0	0.08680008340566674	0.0
25	0.0	4.156428574892262E-4	0.0	0.09324254769674974	0.0
26	0.0	4.156428574892262E-4	0.0	0.10654311913640498	0.0
27	0.0	4.156428574892262E-4	0.0	0.12981911915580166	0.0
28	0.0	5.541904766523016E-4	0.0	0.17685603586166573	0.0
29	0.0	5.541904766523016E-4	0.0	0.24536783353780653	0.0
30	0.0	5.541904766523016E-4	0.0	0.334453952659664	0.0
31	0.0	5.541904766523016E-4	0.0	0.6057994647905471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGGAC	220	0.0	20.181818	7
CGTATGC	195	0.0	18.97436	31
CTCGTAT	200	0.0	18.499998	29
TCGTATG	200	0.0	18.499998	30
ACGGACC	255	0.0	18.137255	8
TCTCGTA	225	0.0	18.088888	28
ATGCCGT	210	0.0	17.619047	34
GCGCAAG	275	0.0	17.490908	1
CTTATGG	75	2.0677279E-4	17.266666	3
ACGAACG	75	2.0677279E-4	17.266666	15
GCGTTAT	130	1.3938916E-8	17.076923	1
CGAATTA	65	0.0015801989	17.076923	15
CGGACCA	265	0.0	16.754717	9
GCGAAAG	255	0.0	16.686274	18
CGAAAGC	270	0.0	16.444445	19
CGTTATT	125	1.6584636E-7	16.279999	2
ATGGGTA	245	0.0	15.857143	5
TTTCGGA	175	1.3096724E-10	15.857142	30
CAAGACG	355	0.0	15.633803	4
GGTATCA	3805	0.0	15.461235	1
>>END_MODULE